Q2NE10 (ILVD_METST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Msp_1576

Organism

Methanosphaera stadtmanae (strain DSM 3091) [Complete proteome] [HAMAP]

Taxonomic identifier

339860 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanosphaera

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000001011
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2NE10 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 8215BC98CDDAD5BD	FASTA	549	59,101
	10 20 30 40 50 60 MRSDNIKKGI NRTSHRSLLR ACGLDDNDMK KPFIGIANSY TDIVPGHIHL KDLVQEVKEV 70 80 90 100 110 120 IRLEGGVPFE FDTMAVCDGI AMNHEGMYYS LPSREIIANT VESMAMAHQF DALILMPTCD 130 140 150 160 170 180 KVVPGMIMAA ARLNIPAIVI TGGPMMPGKF HGETVDFINV TEAVGATQSG KMSEEDLYEL 190 200 210 220 230 240 ERCACPGAGS CAGLFTANTM ACLTETMGMS LPGCATAHAV SDKKVEIARA SAKKIFTLLE 250 260 270 280 290 300 NDIKPSDILT QEAFENAIKV DLALGGSTNT TLHIPAIANE VDGVDVTIDL FDKLSHEVPY 310 320 330 340 350 360 ICSIRPGGNN RMIDVENAGG IPAVMKNMES ILNTDCITCT SNSVKENLEK VSDIDYNVIH 370 380 390 400 410 420 TLDDPIRTEG GIAVLYGNVA PNGCVIKQGA VNEDMLVHSG PCKVFNSEEE CVLAIENDEI 430 440 450 460 470 480 IDGDVVVIRY EGPKGGPGMR EMLNPTAAIM GRELFHVALI TDGRFSGGSR GPCIGHISPE 490 500 510 520 530 540 AAEGGPIGAI ENGDIVSINV KERTIKLELT DDEIKERLSK VKPVERNLKG WLKQYQKLAT SADKGAILK

References

[1]

"The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis." Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R., Gottschalk G., Thauer R.K. J. Bacteriol. 188:642-658(2006) [PubMed: 16385054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 3091.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000102 Genomic DNA. Translation: ABC57943.1.
RefSeq	YP_448586.1. NC_007681.1.
3D structure databases
ProteinModelPortal	Q2NE10.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NE10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3855802.
GenomeReviews	Gene locus Msp_1576 in contig CP000102_GR.
KEGG	mst:Msp_1576.
NMPDR	fig|339860.6.peg.1510.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04340.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q2NE10.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	MSTA339860:MSP_1576-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_METST

Accession

Primary (citable) accession number: Q2NE10

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	November 16, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program