Q2NB87 (Q2NB87_ERYLH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

ELI_04810

Organism

Erythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]

Taxonomic identifier

314225 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Erythrobacteraceae › Erythrobacter

Protein attributes

Sequence length	845 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q2NB87 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 1E0D0F8C40C10C69	FASTA	845	93,938
	10 20 30 40 50 60 MQLVIVESPA KAKTIEKYLG KDFKVLASYG HVRDLPPKDG SVRPDEDFAM DWELYRDKQK 70 80 90 100 110 120 RFKEISDAAK QADRLVLATD PDREGEAISW HVQELLAKRK ALPAKVDRVT FNAITKSAVT 130 140 150 160 170 180 EAMGRPRELD QPLIDAYLAR RALDYLFGFT LSPVLWRKLP GAKSAGRVQS VALRLIVDRE 190 200 210 220 230 240 REIEAFKPEE YWSVLARFEH DGTEFDARMV RFDGKKLDKM TLGNEGSALA AKAAVDAGRF 250 260 270 280 290 300 TVSDIETKPL KRNPAPPFTT STLQQEAARK LGFSASHTMR CAQSLYEAGA ITYMRTDGVQ 310 320 330 340 350 360 MDGSAIAACR HAIEERYESA YLPEKPRFYA TKAKNAQEAH EAIRPTNFNR DSAGSGDEAK 370 380 390 400 410 420 LYGLIFKRAM ASQMATAQLE RTTITLEDPT GQHALRATGQ VVKFPGFFAV YQEGFDDKSD 430 440 450 460 470 480 EDEDGLLPAM HKGDAPAKKG VEANQHFTQP PPRFSEASLV KRLEELGIGR PSTYASTIQT 490 500 510 520 530 540 LRDRDYVRME KNRFFAEESG RLLTAFLERF FPNYVAYEFT AGMEEELDTV SDGREEYKEL 550 560 570 580 590 600 LAKFWADFKP KADEVMEKLP SEVTEVLDDY LSDYLFPPTE DGTEPRACPL CRDEGREGGR 610 620 630 640 650 660 LALRGGRYGA FVACANYPEC KYTRRFAQPG GNESAADDGI MGQHPETGED IERKSGRFGP 670 680 690 700 710 720 YVQMGEGKEA KRASIPKDLD DFDLDWAVKL LDLPRIVGAH PETGKEIEAA IGRYGPYLRH 730 740 750 760 770 780 DGKYGKLQST RDVFEVGMNA AVTILAEAAN RKGGGRGKAE PIKTLGEHPT SGGEMKVMPG 790 800 810 820 830 840 RYGPYVTDGT TNATIPKDMK PEDVSEAQAI ELIDARAAKG PVKKKRKKAP AKKKAAPKKK AAAKK

References

[1]	"Complete genome sequence of Erythrobacter litoralis HTCC2594." Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C. J. Bacteriol. 191:2419-2420(2009) [PubMed: 19168610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000157 Genomic DNA. Translation: ABC63054.1.
RefSeq	YP_457851.1. NC_007722.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NB87.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3869040.
GenomeReviews	Gene locus ELI_04810 in contig CP000157_GR.
KEGG	eli:ELI_04810.
NMPDR	fig|314225.3.peg.2108.
PATRIC	21859036. VBIEryLit102657_0953.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
PhylomeDB	Q2NB87.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q2NB87_ERYLH

Accession

Primary (citable) accession number: Q2NB87

Entry history

Integrated into UniProtKB/TrEMBL:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)