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Q2NB65 (BIOB_ERYLH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:ELI_04920
OrganismErythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]
Taxonomic identifier314225 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesErythrobacteraceaeErythrobacter

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Biotin synthase HAMAP-Rule MF_01694
PRO_0000381382

Sites

Metal binding541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding981Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1291Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1891Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2671Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NB65 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: F81B8CC13D4B7AF2

FASTA34137,045
        10         20         30         40         50         60 
MTPIRTNWSR DEIAALFEQP FTELLFQAAT VHRAYHPPEQ VQLCTLLSIK TGGCPEDCGY 

        70         80         90        100        110        120 
CSQSVKADSG VEATKLMDVQ RVLQSAAQAK DAGSQRFCMG AAWRNPKDRD MPAIVEIVKG 

       130        140        150        160        170        180 
VRDMGLETCM TLGMLTPKQA DMLKDAGLDY YNHNVDTGPE YYERVISTRN YQDRLDTLQN 

       190        200        210        220        230        240 
VRDAGINVCS GGIVGMGETR EDRVGFVHTL ATLERHPESV PVNALVPVKG TVLGDMLADT 

       250        260        270        280        290        300 
PLAKIDDIEF VRTVAVARIT MPLSMVRLSA GRESMSEATQ ALCFMAGANS IFTGDKLLTA 

       310        320        330        340 
ANAGDDKDAA LFDKLGLTAL QGEEPLRRAK DEAGKAAIPA E 

« Hide

References

[1]"Complete genome sequence of Erythrobacter litoralis HTCC2594."
Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C.
J. Bacteriol. 191:2419-2420(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTCC2594.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000157 Genomic DNA. Translation: ABC63076.1.
RefSeqYP_457873.1. NC_007722.1.

3D structure databases

ProteinModelPortalQ2NB65.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING314225.ELI_04920.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC63076; ABC63076; ELI_04920.
GeneID3870147.
KEGGeli:ELI_04920.
PATRIC21859078. VBIEryLit102657_0974.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2391555.

Enzyme and pathway databases

BioCycELIT314225:GHLE-1005-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ERYLH
AccessionPrimary (citable) accession number: Q2NB65
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 7, 2006
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways