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Q2NAD7

- LIPA_ERYLH

UniProt

Q2NAD7 - LIPA_ERYLH

Protein

Lipoyl synthase

Gene

lipA

Organism
Erythrobacter litoralis (strain HTCC2594)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciELIT314225:GHLE-1291-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:ELI_06310
    OrganismiErythrobacter litoralis (strain HTCC2594)
    Taxonomic identifieri314225 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesErythrobacteraceaeErythrobacter
    ProteomesiUP000008808: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320Lipoyl synthasePRO_1000012222Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi314225.ELI_06310.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2NAD7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235998.
    KOiK03644.
    OMAiFERIATN.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2NAD7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGGMNDLSST PAPEGDRPAR QRKPDWIRVK APVSKGYHET RKLMRELNLN    50
    TVCEEAACPN IGECWTKKHA TVMILGDVCT RACAFCNVKT GMPRIVDPME 100
    PENTAIAAAK MGLQHIVITS VDRDDLPDGG AGQFVKVIEA LRRETPDTTI 150
    EILTPDFRGK MRAAVEAICE AGPDVYNHNL ETVPRLYPTI RPGARYYASL 200
    RLLEEVKSHD PMIFTKSGIM LGLGEQRLEV HQVMDDMRSA DVDFITMGQY 250
    LQPTPKHAKV EDFVTPKAFD AFGAIARAKG FLQVASSPLT RSSYHAGDDF 300
    AEMRAAREAK LAKERERAQG 320
    Length:320
    Mass (Da):35,443
    Last modified:February 7, 2006 - v1
    Checksum:i40FCFBDF7307C232
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000157 Genomic DNA. Translation: ABC63354.1.
    RefSeqiWP_011414190.1. NC_007722.1.
    YP_458151.1. NC_007722.1.

    Genome annotation databases

    EnsemblBacteriaiABC63354; ABC63354; ELI_06310.
    GeneIDi3871181.
    KEGGieli:ELI_06310.
    PATRICi21859654. VBIEryLit102657_1254.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000157 Genomic DNA. Translation: ABC63354.1 .
    RefSeqi WP_011414190.1. NC_007722.1.
    YP_458151.1. NC_007722.1.

    3D structure databases

    ProteinModelPortali Q2NAD7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314225.ELI_06310.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABC63354 ; ABC63354 ; ELI_06310 .
    GeneIDi 3871181.
    KEGGi eli:ELI_06310.
    PATRICi 21859654. VBIEryLit102657_1254.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235998.
    KOi K03644.
    OMAi FERIATN.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci ELIT314225:GHLE-1291-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Erythrobacter litoralis HTCC2594."
      Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C.
      J. Bacteriol. 191:2419-2420(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HTCC2594.

    Entry informationi

    Entry nameiLIPA_ERYLH
    AccessioniPrimary (citable) accession number: Q2NAD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3