Reviewed, UniProtKB/Swiss-Prot Q2NAC8 (TRMFO_ERYLH)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	ELI_06355

Organism

Erythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]

Taxonomic identifier

314225 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Erythrobacteraceae › Erythrobacter

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000346338
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NAC8-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 17AC398D39DA5AC4	FASTA	455	50,302
	10 20 30 40 50 60 MSHDVHIIGG GLAGSEAAWQ LARRGVKVRL SEMRGSGEMT HAHQTDGLAE LVCSNSFRSD 70 80 90 100 110 120 DDTKNAVGLL HYEMRALDSL VMRAGEVAQV PAGSAMAVDR DVFSAEVQKT LEAHPNIEVV 130 140 150 160 170 180 RERVDALPEA GLTIVSTGPL TAQSLAESIV KATGQERLAF FDAIAPIVHR DSIDMSKCWI 190 200 210 220 230 240 QSRWNKRTEA SNEDGDYINC PMTKEQYLAF HQGLIDGEKT EFREWEKDTP YFDGCMPIEI 250 260 270 280 290 300 MAARGVETLR YGPMKGVGLD NPYDTSEEHP QGRWPYAVVQ LRQDNKLGTL WNMVGFQTKL 310 320 330 340 350 360 KYGAQVELFR TIPGLENAEF ARLGGLHRNT FLNSPMVLDR QLRLKGAEHI RFAGQVTGCE 370 380 390 400 410 420 GYVESAAVGL MAGMMTAKEL AGDVWQGPPR TTALGALLSH ITGDAEAETY QPMNINFGLF 430 440 450 PPLHEVKKKQ RKEAYTTRAK SDLGEWIAHE VRVSA

References

[1]	Giovannoni S.J., Cho J.-C., Ferriera S., Johnson J., Kravits S., Halpern A., Remington K., Beeson K., Tran B., Rogers Y.-H., Friedman R., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000157 Genomic DNA. Translation: ABC63363.1.
RefSeq	YP_458160.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3871190.
GenomeReviews	Gene locus ELI_06355 in contig CP000157_GR.
KEGG	eli:ELI_06355.
NMPDR	fig|314225.3.peg.1785.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q2NAC8. MKPVGLT.
Enzyme and pathway databases
BioCyc	ELIT314225:ELI_06355-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. [Graphical view]
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_ERYLH

Accession

Primary (citable) accession number: Q2NAC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)