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Q2NAC8 (TRMFO_ERYLH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Ordered Locus Names:ELI_06355
OrganismErythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]
Taxonomic identifier314225 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Erythrobacteraceae › Erythrobacter

Protein attributes

Sequence length455 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037

Cofactor

FAD By similarity. HAMAP MF_01037

Subcellular location

Cytoplasm By similarity HAMAP MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 455455Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP MF_01037
PRO_0000346338

Regions

Nucleotide binding9 – 146FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NAC8 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: 17AC398D39DA5AC4

FASTA45550,302
        10         20         30         40         50         60 
MSHDVHIIGG GLAGSEAAWQ LARRGVKVRL SEMRGSGEMT HAHQTDGLAE LVCSNSFRSD 

        70         80         90        100        110        120 
DDTKNAVGLL HYEMRALDSL VMRAGEVAQV PAGSAMAVDR DVFSAEVQKT LEAHPNIEVV 

       130        140        150        160        170        180 
RERVDALPEA GLTIVSTGPL TAQSLAESIV KATGQERLAF FDAIAPIVHR DSIDMSKCWI 

       190        200        210        220        230        240 
QSRWNKRTEA SNEDGDYINC PMTKEQYLAF HQGLIDGEKT EFREWEKDTP YFDGCMPIEI 

       250        260        270        280        290        300 
MAARGVETLR YGPMKGVGLD NPYDTSEEHP QGRWPYAVVQ LRQDNKLGTL WNMVGFQTKL 

       310        320        330        340        350        360 
KYGAQVELFR TIPGLENAEF ARLGGLHRNT FLNSPMVLDR QLRLKGAEHI RFAGQVTGCE 

       370        380        390        400        410        420 
GYVESAAVGL MAGMMTAKEL AGDVWQGPPR TTALGALLSH ITGDAEAETY QPMNINFGLF 

       430        440        450 
PPLHEVKKKQ RKEAYTTRAK SDLGEWIAHE VRVSA

« Hide

References

[1]"Complete genome sequence of Erythrobacter litoralis HTCC2594."
Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C.
J. Bacteriol. 191:2419-2420(2009) [PubMed: 19168610] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTCC2594.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000157 Genomic DNA. Translation: ABC63363.1.
RefSeqYP_458160.1. NC_007722.1.

3D structure databases

ProteinModelPortalQ2NAC8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2NAC8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3871190.
GenomeReviewsGene locus ELI_06355 in contig CP000157_GR.
KEGGeli:ELI_06355.
NMPDRfig|314225.3.peg.1785.
PATRIC21859674. VBIEryLit102657_1264.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHBG285041.
OMARFAGQIT.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycELIT314225:ELI_06355-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
[Tree]
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
KOK04094.
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. Gid_trmFO. 1 hit.
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_ERYLH
AccessionPrimary (citable) accession number: Q2NAC8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 7, 2006
Last modified: January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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