Q2NA15 (ISPE_ERYLH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	ELI_06920

Organism

Erythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]

Taxonomic identifier

314225 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Erythrobacteraceae › Erythrobacter › Erythrobacter litoralis

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061		PRO_0000335713
Regions
	Nucleotide binding	90 – 100	11	ATP Potential
Sites
	Active site	9	1	By similarity
	Active site	129	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NA15 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 0050FA0530AD3456	FASTA	273	29,217
	10 20 30 40 50 60 MITETAYAKI NLALHVRSKR EDGYHEIETL FAFVDAGDVL VARAAEADRL ETVGEFADVL 70 80 90 100 110 120 DNPFDNLVAR TMSTLPREGG LHVTLEKNLP VAAGLGGGSA DAGAMFRIIE QMHGLPDDWE 130 140 150 160 170 180 EKAIRLGADV PACVASEMAI GRGTGTDLEP VENDMAGMAV LLVNLRVPLP TGPVFKAWAD 190 200 210 220 230 240 TAGGEDLGPL PTGTAREIAR KGRNDLRPPA VAICPPIADV LQALEATDPW MCEMSGSGAT 250 260 270 CFALYDETEM RDKAARAIAE HHPGWWQMTG TLQ

References

[1]	"Complete genome sequence of Erythrobacter litoralis HTCC2594." Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C. J. Bacteriol. 191:2419-2420(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTCC2594.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000157 Genomic DNA. Translation: ABC63476.1.
RefSeq	YP_458273.1. NC_007722.1.
3D structure databases
ProteinModelPortal	Q2NA15.
ModBase	Search...
Protein-protein interaction databases
STRING	314225.ELI_06920.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC63476; ABC63476; ELI_06920.
GeneID	3870896.
KEGG	eli:ELI_06920.
PATRIC	21859898. VBIEryLit102657_1375.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HOG000019600.
KO	K00919.
OMA	VMVNPRV.
ProtClustDB	PRK14608.
Enzyme and pathway databases
BioCyc	ELIT314225:GHLE-1414-MONOMER.
UniPathway	UPA00056; UER00094.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00061. IspE.
InterPro	IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ERYLH

Accession

Primary (citable) accession number: Q2NA15

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 7, 2006
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program