Q2N925 (FABH_ERYLH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	ELI_08620

Organism

Erythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]

Taxonomic identifier

314225 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Erythrobacteraceae › Erythrobacter

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm By similarity HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000187867
Regions
	Region	249 – 253	5	ACP-binding By similarity
Sites
	Active site	113	1	By similarity
	Active site	248	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2N925 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 5176C28AE2F742FC	FASTA	321	34,025
	10 20 30 40 50 60 MIRAAIKGSG SALPRKVVTN EELAARVDTS DEWIVERTGI RQRYIAGADE FTSTLAMEAS 70 80 90 100 110 120 RKALEAAGIT PETIDLIVLA TATPDNTFPA TATKVQEALG CNGCIAFDVQ AVCSGFLYAL 130 140 150 160 170 180 ATADSLLKTG MAKRALVIGA ETFSRILDWE DRTTCVLFGD GAGAVVLEAA EGDTEAPGIL 190 200 210 220 230 240 ASRLHADGAQ HDLLYVDGGP STTQTVGHLR MRGREVFRHA VVNLADVLKE VLDDAGVSAS 250 260 270 280 290 300 DIDWVVPHQA NARILDATAR KLGLPMEKIV VTVDRHANTS AASVPLAFDT AVRDGRIKPG 310 320 DLVMFEAMGG GFTWGASLVR Y

References

[1]	"Complete genome sequence of Erythrobacter litoralis HTCC2594." Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C. J. Bacteriol. 191:2419-2420(2009) [PubMed: 19168610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTCC2594.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000157 Genomic DNA. Translation: ABC63816.1.
RefSeq	YP_458613.1. NC_007722.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2N925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3871099.
GenomeReviews	Gene locus ELI_08620 in contig CP000157_GR.
KEGG	eli:ELI_08620.
NMPDR	fig|314225.3.peg.1318.
PATRIC	21860574. VBIEryLit102657_1710.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0332.
HOGENOM	HBG649927.
OMA	DAYIRGG.
PhylomeDB	Q2N925.
ProtClustDB	CLSK844009.
Enzyme and pathway databases
BioCyc	ELIT314225:ELI_08620-MONOMER.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_ERYLH

Accession

Primary (citable) accession number: Q2N925

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program