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Q2N784

- GLND_ERYLH

UniProt

Q2N784 - GLND_ERYLH

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Erythrobacter litoralis (strain HTCC2594)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciELIT314225:GHLE-2407-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:ELI_11825
OrganismiErythrobacter litoralis (strain HTCC2594)
Taxonomic identifieri314225 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesErythrobacteraceaeErythrobacter
ProteomesiUP000008808: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022343Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi314225.ELI_11825.

Structurei

3D structure databases

ProteinModelPortaliQ2N784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini490 – 592103HDUniRule annotationAdd
BLAST
Domaini728 – 81184ACT 1UniRule annotationAdd
BLAST
Domaini839 – 91981ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 373373UridylyltransferaseAdd
BLAST
Regioni374 – 727354Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR005105. GlnD_Uridyltrans_N.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF03445. DUF294. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2N784-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDPKVPRQR RIIDRRKLAA AVEALAEQQG EKARPAVLKV LREALEKGRD
60 70 80 90 100
ELSQRLLDRP SAGHQITAGH AFLVDQLVRV IFDHVTTHLY PVANRSSSER
110 120 130 140 150
IAVLAVGGYG RAEMAPQSDV DIAFLHPSRR TPWCEQVTEA MLYFLWDLGF
160 170 180 190 200
KVGQSSRTPE DMVRMAREDL TIRTALLEAR FVWGDRELYD EARKRFWSEV
210 220 230 240 250
VNGTERQFVA EKLAERDARH ERMGGTRYVV EPNVKEGKGG LRDLQTLYWI
260 270 280 290 300
GKYIHRARGA AELVDAGLLT ETEYHGFRRA EGFLLAVRCH LHEITGRPED
310 320 330 340 350
RLTFDFQKQI AERMRFAERR EKSAVERFMQ YYFLQVKRVG SLTGVFLAQM
360 370 380 390 400
DQQFARKRAR TGLLAGFNAK SRMLKGYTVF GGKIAAPGDN WFRDDPVRLI
410 420 430 440 450
EIFQLAEANG LEIDPRSMRQ ADRDSVLIKD QVRNDPRANA IFLDLLCGRN
460 470 480 490 500
DPETALRWMN EAGVFGKFVP DFGRVNAQMQ FNMYHHYTVD EHTIRAIGFL
510 520 530 540 550
SKIEKGELAK EHPRSTREIH KVKSRRVAFV AALLHDIAKG RGGDHSILGA
560 570 580 590 600
EVAEELCPRF GLDEDETDLV AWLVLQHLLM SSTAQKRDLT DPKTIEDFVA
610 620 630 640 650
EVQSLERLRH LAILTSVDIR AVGPGTWNSW KGQLLGELYD AAHERLRLGH
660 670 680 690 700
MKHHRSERVA AKKEAVREAL GGKAALLEKH GRLLPDSYWI AEPENVISRN
710 720 730 740 750
IVQYDVAREI SEDLSIHCEF DEERGATLVT VIAADHPGLF YRIAGGIHLA
760 770 780 790 800
GGNIIDARIH TTRNGWAIDN YLVQDPVGQP FAEERQLARI EQAIADAIAN
810 820 830 840 850
RGELVPKLAK RPLKQTRAGA FDVRPRVLFD NDASGRFTVI EVNARDRAAL
860 870 880 890 900
LNRLGRALFE NQVIVQSAHI TAYGERAADT FYVTDLTGAK ITDESRMDTI
910
RQALLDAASD ARQAELEPA
Length:919
Mass (Da):104,003
Last modified:February 7, 2006 - v1
Checksum:iB532D60CE3188D12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000157 Genomic DNA. Translation: ABC64457.1.
RefSeqiYP_459254.1. NC_007722.1.

Genome annotation databases

EnsemblBacteriaiABC64457; ABC64457; ELI_11825.
GeneIDi3871505.
KEGGieli:ELI_11825.
PATRICi21861869. VBIEryLit102657_2349.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000157 Genomic DNA. Translation: ABC64457.1 .
RefSeqi YP_459254.1. NC_007722.1.

3D structure databases

ProteinModelPortali Q2N784.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314225.ELI_11825.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABC64457 ; ABC64457 ; ELI_11825 .
GeneIDi 3871505.
KEGGi eli:ELI_11825.
PATRICi 21861869. VBIEryLit102657_2349.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci ELIT314225:GHLE-2407-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR005105. GlnD_Uridyltrans_N.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF03445. DUF294. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Erythrobacter litoralis HTCC2594."
    Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C.
    J. Bacteriol. 191:2419-2420(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTCC2594.

Entry informationi

Entry nameiGLND_ERYLH
AccessioniPrimary (citable) accession number: Q2N784
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: November 26, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3