Q2N5X1 (T23O_ERYLH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	ELI_14140

Organism

Erythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]

Taxonomic identifier

314225 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Erythrobacteraceae › Erythrobacter

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Tryptophan 2,3-dioxygenase		PRO_0000360114
Regions
	Region	7 – 11	5	Substrate binding By similarity
	Region	32 – 36	5	Substrate binding By similarity
Sites
	Metal binding	221	1	Iron (heme axial ligand) By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	Heme By similarity
	Binding site	235	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2N5X1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 35222157E9DEDA18	FASTA	263	30,341
	10 20 30 40 50 60 MAQDVTYSSY LDLDRILAAQ HPVSGAHDEM LFIIVHQASE LWLKLCLHEL FAARDCIAAD 70 80 90 100 110 120 NLRPSFKMLS RVARAQTQLI QSWDVLSTMT PHDYSQIRPH LGRSSGFQSP QYRMMEFLLG 130 140 150 160 170 180 GRNPDMVAMH EPTPEIATAL REELARTSLY DEAIRLLSRR GFAIPDEVLA RKLDEVWVRS 190 200 210 220 230 240 EEVEAAWAEI YRDPQQHWDL YELAEKLVDL EYHFQRWRFG HLKTVERIIG FKRGTGGTKG 250 260 VPYLEGVLKQ AFFPELLSVR TAI

References

[1]	"Complete genome sequence of Erythrobacter litoralis HTCC2594." Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C. J. Bacteriol. 191:2419-2420(2009) [PubMed: 19168610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTCC2594.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000157 Genomic DNA. Translation: ABC64920.1.
RefSeq	YP_459717.1. NC_007722.1.
3D structure databases
ProteinModelPortal	Q2N5X1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2N5X1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3871638.
GenomeReviews	Gene locus ELI_14140 in contig CP000157_GR.
KEGG	eli:ELI_14140.
NMPDR	fig|314225.3.peg.190.
PATRIC	21862835. VBIEryLit102657_2828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	QYREIEF.
Enzyme and pathway databases
BioCyc	ELIT314225:ELI_14140-MONOMER.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_ERYLH

Accession

Primary (citable) accession number: Q2N5X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program