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Q2N5V5 (TRMB_ERYLH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:ELI_14220
OrganismErythrobacter litoralis (strain HTCC2594) [Complete proteome] [HAMAP]
Taxonomic identifier314225 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Erythrobacteraceae › Erythrobacter ›

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288146

Regions

Region211 – 2144Substrate binding Potential

Sites

Binding site621S-adenosyl-L-methionine By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1141S-adenosyl-L-methionine By similarity
Binding site1361S-adenosyl-L-methionine By similarity
Binding site1401Substrate By similarity
Binding site1721Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q2N5V5 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: 49B7EFD4AE3F780E

FASTA23326,810
        10         20         30         40         50         60 
MTAFKQGDPT TLNRLYGRSI GKPLRTHQQH LVDNLLPQIS PPAEGPVTAE RLFGEDCPLH 

        70         80         90        100        110        120 
FEIGFGGGEH LVYRADLLPN HGFIGAEPFL NGVASCLSQV EEQRLANIRI HNGDALEVLD 

       130        140        150        160        170        180 
RVPDGALTML YLLHPDPWPK NKHAKRRMMN DGPVKMFADK LKPGGEFRFG TDHPVYLRHA 

       190        200        210        220        230 
LMVMRRHTDA FEWVVEGPSD WQNRPSGWPE TRYEHKARTK FGHEVWYFRF RRR

« Hide

References

[1]"Complete genome sequence of Erythrobacter litoralis HTCC2594."
Oh H.M., Giovannoni S.J., Ferriera S., Johnson J., Cho J.C.
J. Bacteriol. 191:2419-2420(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTCC2594.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000157 Genomic DNA. Translation: ABC64936.1.
RefSeqYP_459733.1. NC_007722.1.

3D structure databases

ProteinModelPortalQ2N5V5.
ModBaseSearch...

Protein-protein interaction databases

STRING314225.ELI_14220.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC64936; ABC64936; ELI_14220.
GeneID3870854.
KEGGeli:ELI_14220.
PATRIC21862865. VBIEryLit102657_2843.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMALYPDPWP.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycELIT314225:GHLE-2890-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ERYLH
AccessionPrimary (citable) accession number: Q2N5V5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 7, 2006
Last modified: May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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