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Protein

Myocyte-specific enhancer factor 2A

Gene

Mef2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter (By similarity).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi58 – 86Mef2-typeSequence analysisAdd BLAST29

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cellular response to calcium ion Source: UniProtKB
  • cellular response to glucose stimulus Source: RGD
  • dendrite morphogenesis Source: UniProtKB
  • ERK5 cascade Source: UniProtKB
  • MAPK cascade Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processApoptosis, Differentiation, Neurogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-198753. ERK/MAPK targets.

Names & Taxonomyi

Protein namesi
Recommended name:
Myocyte-specific enhancer factor 2A
Gene namesi
Name:Mef2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1359360. Mef2a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi395K → R: Abolishes sumoylation. 1 Publication1
Mutagenesisi400S → A: Abolishes K-395 sumoylation. Enhances K-395 acetylation. Enhances transcriptional activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669701 – 495Myocyte-specific enhancer factor 2AAdd BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59Phosphoserine; by CK2By similarity1
Modified residuei98PhosphoserineCombined sources1
Modified residuei235PhosphoserineCombined sources1
Modified residuei249N6-acetyllysineBy similarity1
Modified residuei255PhosphoserineCombined sources1
Modified residuei304Phosphothreonine; by MAPK7 and MAPK14By similarity1
Modified residuei311Phosphothreonine; by MAPK7 and MAPK14By similarity1
Modified residuei347Phosphoserine; by MAPK7By similarity1
Modified residuei395N6-acetyllysine; alternate1 Publication1
Cross-linki395Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Modified residuei400Phosphoserine; by CDK51 Publication1
Modified residuei407PhosphothreonineBy similarity1
Modified residuei441PhosphoserineBy similarity1

Post-translational modificationi

Constitutive phosphorylation on Ser-400 promotes Lys-395 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-400 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-395 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-304 and Thr-311 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-400 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-304, Thr-311 and Ser-347 can be induced by EGF (By similarity).By similarity
Sumoylation on Lys-395 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-400 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated with SUMO1 and, to a lesser extent with SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis (By similarity).By similarity
Acetylation on Lys-395 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.By similarity1 Publication
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei213 – 214CleavageCurated2
Sitei454 – 455CleavageCurated2

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ2MJT0.
PRIDEiQ2MJT0.

PTM databases

iPTMnetiQ2MJT0.
PhosphoSitePlusiQ2MJT0.

Interactioni

Subunit structurei

Binds DNA as a homo- or heterodimer (By similarity). Dimerizes with MEF2D. Interacts with HDAC7. Interacts with PIAS1; the interaction enhances sumoylation. Interacts with HDAC4, HDAC9 and SLC2A4RG. Interacts (via the N-terminal) with MAPK7; the interaction results in the phosphorylation and transcriptional activity of MEF2A (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Habp4A1L1K83EBI-7612509,EBI-9825803

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ2MJT0. 2 interactors.
MINTiMINT-1346606.
STRINGi10116.ENSRNOP00000065122.

Structurei

3D structure databases

ProteinModelPortaliQ2MJT0.
SMRiQ2MJT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 283Required for interaction with MAPKsBy similarityAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi256 – 259Poly-Pro4
Compositional biasi412 – 436Gln/Pro-richAdd BLAST25

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOVERGENiHBG053944.
InParanoidiQ2MJT0.
KOiK09260.
PhylomeDBiQ2MJT0.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
Gene3Di3.40.1810.10. 1 hit.
InterProiView protein in InterPro
IPR022102. HJURP_C.
IPR033896. MADS_MEF2-like.
IPR002100. TF_MADSbox.
PfamiView protein in Pfam
PF12347. HJURP_C. 1 hit.
PF00319. SRF-TF. 1 hit.
PRINTSiPR00404. MADSDOMAIN.
SMARTiView protein in SMART
SM00432. MADS. 1 hit.
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiView protein in PROSITE
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2MJT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKKIQITR IMDERNRQVT FTKRKFGLMK KAYELSVLCD CEIALIIFNS
60 70 80 90 100
SNKLFQYAST DMDKVLLKYT EYNEPHESRT NSDIVEALNK KEHRGCDSPD
110 120 130 140 150
PDTSYVLTPH TEEKYKKINE EFDNMMRNHK IAPGLPPQNF SMSVTVPVTS
160 170 180 190 200
PNALSYTNPG SSLVSPSLAA SSTLAESSML SPPPATLHRN VSPGAPQRPP
210 220 230 240 250
STGSAGGMLS TTDLTVPNGA GNGPVGNGFV DSRASPNLIG NTGANSVGKV
260 270 280 290 300
MPTKSPPPPG GGSVGMNSRK PDLRVVIPPS SKGMMPPLNA QRISSSQATQ
310 320 330 340 350
PLATPVVSVT TPSLPPQGLV YSAMPTAYNT DYSLTSADLS ALQGFTSPGM
360 370 380 390 400
LSLGQASAWQ QHHLGQAALS SLVAGGQLSQ GSNLSINTNQ NINIKTEPIS
410 420 430 440 450
PPRDRMTPSG FQQQQQQQPQ QQPPPQPPQP QPQPRQEMGR SPVDSLSSSS
460 470 480 490
SSYDGSDRED PRGDFHSPIV LGRPPNAEDR ESPSVKRMRM DTWVT
Length:495
Mass (Da):53,254
Last modified:February 7, 2006 - v1
Checksum:i8ED69528402E613E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223G → S in AAH81907 (PubMed:15489334).Curated1
Sequence conflicti231D → N in AAH81907 (PubMed:15489334).Curated1
Sequence conflicti396T → S in AAH81907 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ323505 mRNA. Translation: ABC55063.1.
BC081907 mRNA. Translation: AAH81907.1.
RefSeqiNP_001014057.1. NM_001014035.1.
XP_017444797.1. XM_017589308.1.
UniGeneiRn.162435.

Genome annotation databases

GeneIDi309957.
KEGGirno:309957.

Similar proteinsi

Entry informationi

Entry nameiMEF2A_RAT
AccessioniPrimary (citable) accession number: Q2MJT0
Secondary accession number(s): Q66HD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 7, 2006
Last modified: September 27, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome