Q2MJR0 (SPRE3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sprouty-related, EVH1 domain-containing protein 3
Short name=Spred-3

Gene names

Name:	SPRED3
Synonyms:	EVE-3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase By similarity.
Subunit structure	Interacts with Ras By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expression is restricted to liver. Ref.1
Post-translational modification	Phosphorylated on tyrosine By similarity.
Sequence similarities	Contains 1 KBD domain. Contains 1 SPR (sprouty) domain. Contains 1 WH1 domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inactivation of MAPK activity Inferred from electronic annotation. Source: Compara multicellular organismal development Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q2MJR0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q2MJR0-2)

Also known as: EVE-3;

The sequence of this isoform differs from the canonical sequence as follows:
     142-152: SHVDSDSSSSH → LSQYFRHMLCP
     153-410: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Sprouty-related, EVH1 domain-containing protein 3		PRO_0000247431
Regions
	Domain	1 – 113	113	WH1
	Domain	195 – 244	50	KBD
	Domain	296 – 407	112	SPR
	Compositional bias	117 – 153	37	Ser-rich
	Compositional bias	254 – 289	36	Pro-rich
Natural variations
	Alternative sequence	142 – 152	11	SHVDSDSSSSH → LSQYFRHMLCP in isoform 2.		VSP_042949
	Alternative sequence	153 – 410	258	Missing in isoform 2.		VSP_042950

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 5086B02CAA11BDFF	FASTA	410	42,670
	10 20 30 40 50 60 MVRVRAVVMA RDDSSGGWLP VGGGGLSQVS VCRVRGARPE GGARQGHYVI HGERLRDQKT 70 80 90 100 110 120 TLECTLKPGL VYNKVNPIFH HWSLGDCKFG LTFQSPAEAD EFQKSLLAAL AALGRGSLTP 130 140 150 160 170 180 SSSSSSSSPS QDTAETPCPL TSHVDSDSSS SHSRQETPPS AAAAPIITME SASGFGPTTP 190 200 210 220 230 240 PQRRRSSAQS YPPLLPFTGI PEPSEPLAGA GGLGWGGRGY EDYRRSGPPA PLALSTCVVR 250 260 270 280 290 300 FAKTGALRGA ALGPPAALPA PLTEAAPPAP PARPPPGPGP SSAPAKASPE AEEAARCVHC 310 320 330 340 350 360 RALFRRRADG RGGRCAEAPD PGRLLVRRLS CLWCAESLLY HCLSDAEGDF SDPCACEPGH 370 380 390 400 410 PRPAARWAAL AALSLAVPCL CCYAPLRACH WVAARCGCAG CGGRHEEAAR
	Isoform 2 (EVE-3) [UniParc]. Checksum: FC092C3286B36463	FASTA	152	16,330
	10 20 30 40 50 60 MVRVRAVVMA RDDSSGGWLP VGGGGLSQVS VCRVRGARPE GGARQGHYVI HGERLRDQKT 70 80 90 100 110 120 TLECTLKPGL VYNKVNPIFH HWSLGDCKFG LTFQSPAEAD EFQKSLLAAL AALGRGSLTP 130 140 150 SSSSSSSSPS QDTAETPCPL TLSQYFRHML CP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Eve-3: a liver enriched suppressor of Ras/MAPK signaling." King J.A.J., Corcoran N.M., D'Abaco G.M., Straffon A.F., Smith C.T., Poon C.L.C., Buchert M., I S.T.T., Hall N.E., Lock P., Hovens C.M. J. Hepatol. 44:758-767(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Tissue: Liver.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ323927 mRNA. Translation: ABC54711.1. DQ323928 mRNA. Translation: ABC54712.1. AC005789 Genomic DNA. No translation available.
IPI	IPI00397716. IPI00783043.
RefSeq	NP_001035987.1. NM_001042522.2.
UniGene	Hs.196011.
3D structure databases
ProteinModelPortal	Q2MJR0.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000345405.
PTM databases
PhosphoSite	Q2MJR0.
Polymorphism databases
DMDM	110816429.
Proteomic databases
PaxDb	Q2MJR0.
PRIDE	Q2MJR0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338502; ENSP00000345405; ENSG00000188766. ENST00000396877; ENSP00000380086; ENSG00000188766. ENST00000586301; ENSP00000466568; ENSG00000188766.
GeneID	399473.
KEGG	hsa:399473.
UCSC	uc002oil.1. human. uc002oim.3. human.
Organism-specific databases
CTD	399473.
GeneCards	GC19P038880.
HGNC	HGNC:31041. SPRED3.
MIM	609293. gene.
neXtProt	NX_Q2MJR0.
PharmGKB	PA134871045.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG297893.
HOGENOM	HOG000220886.
HOVERGEN	HBG057556.
InParanoid	Q2MJR0.
KO	K04703.
OMA	EAARCVH.
OrthoDB	EOG4VMFG7.
Gene expression databases
Bgee	Q2MJR0.
CleanEx	HS_SPRED3.
Genevestigator	Q2MJR0.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR000697. EVH1. IPR023337. KBD. IPR011993. PH_like_dom. IPR007875. Sprouty. [Graphical view]
Pfam	PF05210. Sprouty. 1 hit. PF00568. WH1. 1 hit. [Graphical view]
PROSITE	PS51488. KBD. 1 hit. PS51227. SPR. 1 hit. PS50229. WH1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	399473.
NextBio	105420.
SOURCE	Search...

Entry information

Entry name

SPRE3_HUMAN

Accession

Primary (citable) accession number: Q2MJR0
Secondary accession number(s): Q2MJR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	February 7, 2006
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.