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Protein

Spermatid-specific manchette-related protein 1

Gene

Smrp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in differentiation or function of ciliated cells (By similarity). May play a role in spermatogenesis.By similarity1 Publication

GO - Molecular functioni

  • alpha-tubulin binding Source: MGI

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cellular protein complex assembly Source: MGI
  • spermatogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatid-specific manchette-related protein 1
Alternative name(s):
Ciliated bronchial epithelial protein 1
Gene namesi
Name:Smrp1
Synonyms:Cbe1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1920971. 1110017D15Rik.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • manchette Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Spermatid-specific manchette-related protein 1PRO_0000296260Add
BLAST

Proteomic databases

PaxDbiQ2MH31.
PRIDEiQ2MH31.
TopDownProteomicsiQ2MH31-1. [Q2MH31-1]

PTM databases

PhosphoSiteiQ2MH31.

Expressioni

Tissue specificityi

Isoforms 1 is testis-specific where it is expressed exclusively in germ cells (at protein level). Isoform 2 is testis-specific. Isoform 3 and isoform 4 are expressed in both lung and testis.2 Publications

Developmental stagei

During male germ cell development, expression of isoform 1 begins at day 21, increases after day 30 and continues to increase in the adult. Expressed in elongating spermatids from steps 9-12 (at protein level). Expression in the lung increases at E16.2 Publications

Gene expression databases

BgeeiQ2MH31.
CleanExiMM_1110017D15RIK.
ExpressionAtlasiQ2MH31. baseline and differential.
GenevisibleiQ2MH31. MM.

Interactioni

Subunit structurei

Interacts with alpha-tubulin.1 Publication

GO - Molecular functioni

  • alpha-tubulin binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030152.

Family & Domainsi

Sequence similaritiesi

Belongs to the SMRP1 family.Curated

Phylogenomic databases

eggNOGiENOG410IJMT. Eukaryota.
ENOG41119UP. LUCA.
GeneTreeiENSGT00390000000945.
HOGENOMiHOG000111634.
HOVERGENiHBG095599.
InParanoidiQ2MH31.
KOiK18634.
OMAiCAWEANK.
PhylomeDBiQ2MH31.
TreeFamiTF335656.

Family and domain databases

InterProiIPR028195. SMRP1.
[Graphical view]
PfamiPF15181. SMRP1. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2MH31-1) [UniParc]FASTAAdd to basket

Also known as: Cbe1-long-ORF1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFLFSRKTKT PISTYSDSYR APTSIKEVYK DPPLWAWEAN KFVTPGLTQT
60 70 80 90 100
MHRHVDPEAL QKMTKCAAQD YTYKSSISGH PYLPEKYWLS PDEEDKCCPS
110 120 130 140 150
YLDNDRYNTW KTSPCSNYWN KYTGCLPRLS KDTGMESVRG MPLEYPPKQE
160 170 180 190 200
RLNAYEREVV VNMLNSLSRN RTLPQIVPRC GCVDPLPGRL PYQGYESPCS
210 220 230 240 250
GRHYCLRGMD YCTTREPSTE RRLRPLCSQQ PTECVALRSP ARNAMCCYNS
260
PAIILPVSQP
Length:260
Mass (Da):29,939
Last modified:February 21, 2006 - v1
Checksum:i3BAE6877F4490A0E
GO
Isoform 2 (identifier: Q2MH31-4) [UniParc]FASTAAdd to basket

Also known as: Cbe1-long-ORF2

The sequence of this isoform differs from the canonical sequence as follows:
     233-260: ECVALRSPARNAMCCYNSPAIILPVSQP → VRSVSPFGHR...VSETCPVYPS

Show »
Length:296
Mass (Da):34,175
Checksum:iEDC0F7E430F53285
GO
Isoform 3 (identifier: Q2MH31-2) [UniParc]FASTAAdd to basket

Also known as: Cbe1-ORF1

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: Missing.

Show »
Length:126
Mass (Da):14,317
Checksum:i820295EE000B1F69
GO
Isoform 4 (identifier: Q2MH31-3) [UniParc]FASTAAdd to basket

Also known as: Cbe1-ORF2

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: Missing.
     233-260: ECVALRSPARNAMCCYNSPAIILPVSQP → VRSVSPFGHR...VSETCPVYPS

Show »
Length:162
Mass (Da):18,552
Checksum:i88502950CD0733A6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 134134Missing in isoform 3 and isoform 4. 2 PublicationsVSP_027172Add
BLAST
Alternative sequencei233 – 26028ECVAL…PVSQP → VRSVSPFGHRPGMQCAVTTP PPSFYPYPNLRWDTSHFKKT GGSQRNNYVVHPEFVSETCP VYPS in isoform 2 and isoform 4. 1 PublicationVSP_027173Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB231294 mRNA. Translation: BAE78528.1.
DQ873295 mRNA. Translation: ABI35956.1.
DQ873296 mRNA. Translation: ABI35957.1.
AK003742 mRNA. Translation: BAB22974.1.
AL831723 Genomic DNA. No translation available.
CCDSiCCDS38717.1. [Q2MH31-1]
RefSeqiNP_001041470.1. NM_001048005.1. [Q2MH31-1]
NP_001240706.1. NM_001253777.1. [Q2MH31-4]
NP_001240707.1. NM_001253778.1. [Q2MH31-3]
NP_082900.1. NM_028624.1. [Q2MH31-2]
UniGeneiMm.45614.

Genome annotation databases

EnsembliENSMUST00000030152; ENSMUSP00000030152; ENSMUSG00000028441. [Q2MH31-1]
GeneIDi73721.
KEGGimmu:73721.
UCSCiuc008siu.1. mouse. [Q2MH31-2]
uc008siv.1. mouse. [Q2MH31-3]
uc008six.1. mouse. [Q2MH31-1]
uc008siy.1. mouse. [Q2MH31-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB231294 mRNA. Translation: BAE78528.1.
DQ873295 mRNA. Translation: ABI35956.1.
DQ873296 mRNA. Translation: ABI35957.1.
AK003742 mRNA. Translation: BAB22974.1.
AL831723 Genomic DNA. No translation available.
CCDSiCCDS38717.1. [Q2MH31-1]
RefSeqiNP_001041470.1. NM_001048005.1. [Q2MH31-1]
NP_001240706.1. NM_001253777.1. [Q2MH31-4]
NP_001240707.1. NM_001253778.1. [Q2MH31-3]
NP_082900.1. NM_028624.1. [Q2MH31-2]
UniGeneiMm.45614.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030152.

PTM databases

PhosphoSiteiQ2MH31.

Proteomic databases

PaxDbiQ2MH31.
PRIDEiQ2MH31.
TopDownProteomicsiQ2MH31-1. [Q2MH31-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030152; ENSMUSP00000030152; ENSMUSG00000028441. [Q2MH31-1]
GeneIDi73721.
KEGGimmu:73721.
UCSCiuc008siu.1. mouse. [Q2MH31-2]
uc008siv.1. mouse. [Q2MH31-3]
uc008six.1. mouse. [Q2MH31-1]
uc008siy.1. mouse. [Q2MH31-4]

Organism-specific databases

MGIiMGI:1920971. 1110017D15Rik.

Phylogenomic databases

eggNOGiENOG410IJMT. Eukaryota.
ENOG41119UP. LUCA.
GeneTreeiENSGT00390000000945.
HOGENOMiHOG000111634.
HOVERGENiHBG095599.
InParanoidiQ2MH31.
KOiK18634.
OMAiCAWEANK.
PhylomeDBiQ2MH31.
TreeFamiTF335656.

Miscellaneous databases

NextBioi338893.
PROiQ2MH31.
SOURCEiSearch...

Gene expression databases

BgeeiQ2MH31.
CleanExiMM_1110017D15RIK.
ExpressionAtlasiQ2MH31. baseline and differential.
GenevisibleiQ2MH31. MM.

Family and domain databases

InterProiIPR028195. SMRP1.
[Graphical view]
PfamiPF15181. SMRP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the spermatid-specific Smrp1 gene encoding a novel manchette protein."
    Matsuoka Y., Miyagawa Y., Tokuhiro K., Kitamura K., Iguchi N., Maekawa M., Takahashi T., Tsujimura A., Matsumiya K., Okuyama A., Nishimune Y., Tanaka H.
    Mol. Reprod. Dev. 75:967-975(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH ALPHA-TUBULIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Testis.
  2. "Chronological expression of Ciliated Bronchial Epithelium 1 during pulmonary development."
    Haitchi H.M., Yoshisue H., Ribbene A., Wilson S.J., Holloway J.W., Bucchieri F., Hanley N.A., Wilson D.I., Zummo G., Holgate S.T., Davies D.E.
    Eur. Respir. J. 33:1095-1104(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), ALTERNATIVE SPLICING (ISOFORMS 1; 2; 3 AND 4), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: MF1.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiSMRP1_MOUSE
AccessioniPrimary (citable) accession number: Q2MH31
Secondary accession number(s): Q0GC36, Q9D1B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: February 21, 2006
Last modified: March 16, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.