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Protein

Hyaluronidase-4

Gene

HYAL4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. Has also chondroitin sulfate hydrolase activity, The best substrate being the galactosaminidic linkage in the sequence of a trisulfated tetrasaccharide.2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

pH dependencei

Optimum pH is 4.5-5 (for chondroitin sulfate hydrolase activity).1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei147Proton donorBy similarity1

GO - Molecular functioni

  • hyalurononglucosaminidase activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:HS02883-MONOMER.
ZFISH:HS02883-MONOMER.
BRENDAi3.2.1.35. 2681.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-4 (EC:3.2.1.35)
Short name:
Hyal-4
Alternative name(s):
Chondroitin sulfate endo-beta-N-acetylgalactosaminidase
Chondroitin sulfate hydrolase
Short name:
CSHY
Hyaluronoglucosaminidase-4
Gene namesi
Name:HYAL4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:5323. HYAL4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 453ExtracellularSequence analysisAdd BLAST424
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 481CytoplasmicSequence analysis7

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi23553.
OpenTargetsiENSG00000106302.
PharmGKBiPA29574.

Polymorphism and mutation databases

BioMutaiHYAL4.
DMDMi158564281.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003019991 – 481Hyaluronidase-4Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi59 ↔ 351By similarity
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Glycosylationi115N-linked (GlcNAc...)Sequence analysis1
Glycosylationi177N-linked (GlcNAc...) (complex)1 Publication1
Disulfide bondi223 ↔ 237By similarity
Glycosylationi343N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Disulfide bondi437 ↔ 446By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ2M3T9.
PeptideAtlasiQ2M3T9.
PRIDEiQ2M3T9.

PTM databases

iPTMnetiQ2M3T9.
PhosphoSitePlusiQ2M3T9.

Expressioni

Tissue specificityi

Detected in placenta and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000106302.
CleanExiHS_HYAL4.
ExpressionAtlasiQ2M3T9. baseline and differential.
GenevisibleiQ2M3T9. HS.

Organism-specific databases

HPAiHPA029453.

Interactioni

Protein-protein interaction databases

BioGridi117097. 3 interactors.
STRINGi9606.ENSP00000223026.

Structurei

3D structure databases

ProteinModelPortaliQ2M3T9.
SMRiQ2M3T9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

EGF-like domain, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IECJ. Eukaryota.
ENOG410XPZT. LUCA.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ2M3T9.
KOiK01197.
OMAiSEDGEFT.
OrthoDBiEOG091G064G.
PhylomeDBiQ2M3T9.
TreeFamiTF321598.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2M3T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLSEGQLK LCVVQPVHLT SWLLIFFILK SISCLKPARL PIYQRKPFIA
60 70 80 90 100
AWNAPTDQCL IKYNLRLNLK MFPVIGSPLA KARGQNVTIF YVNRLGYYPW
110 120 130 140 150
YTSQGVPING GLPQNISLQV HLEKADQDIN YYIPAEDFSG LAVIDWEYWR
160 170 180 190 200
PQWARNWNSK DVYRQKSRKL ISDMGKNVSA TDIEYLAKVT FEESAKAFMK
210 220 230 240 250
ETIKLGIKSR PKGLWGYYLY PDCHNYNVYA PNYSGSCPED EVLRNNELSW
260 270 280 290 300
LWNSSAALYP SIGVWKSLGD SENILRFSKF RVHESMRIST MTSHDYALPV
310 320 330 340 350
FVYTRLGYRD EPLFFLSKQD LVSTIGESAA LGAAGIVIWG DMNLTASKAN
360 370 380 390 400
CTKVKQFVSS DLGSYIANVT RAAEVCSLHL CRNNGRCIRK MWNAPSYLHL
410 420 430 440 450
NPASYHIEAS EDGEFTVKGK ASDTDLAVMA DTFSCHCYQG YEGADCREIK
460 470 480
TADGCSGVSP SPGSLMTLCL LLLASYRSIQ L
Length:481
Mass (Da):54,249
Last modified:September 11, 2007 - v2
Checksum:i9D530009AA898D1F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti263G → C in AAC98883 (PubMed:10493834).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034936346A → S.1 PublicationCorresponds to variant rs6949082dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009010 mRNA. Translation: AAC98883.1.
AB470346 mRNA. Translation: BAI49593.1.
AC006029 Genomic DNA. Translation: AAD43186.1.
CH236947 Genomic DNA. Translation: EAL24331.1.
CH471070 Genomic DNA. Translation: EAW83603.1.
BC104788 mRNA. Translation: AAI04789.1.
BC104790 mRNA. Translation: AAI04791.1.
CCDSiCCDS5789.1.
RefSeqiNP_036401.2. NM_012269.2.
XP_011514292.1. XM_011515990.2.
XP_016867400.1. XM_017011911.1.
UniGeneiHs.28673.

Genome annotation databases

EnsembliENST00000223026; ENSP00000223026; ENSG00000106302.
ENST00000476325; ENSP00000417186; ENSG00000106302.
GeneIDi23553.
KEGGihsa:23553.
UCSCiuc003vlc.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009010 mRNA. Translation: AAC98883.1.
AB470346 mRNA. Translation: BAI49593.1.
AC006029 Genomic DNA. Translation: AAD43186.1.
CH236947 Genomic DNA. Translation: EAL24331.1.
CH471070 Genomic DNA. Translation: EAW83603.1.
BC104788 mRNA. Translation: AAI04789.1.
BC104790 mRNA. Translation: AAI04791.1.
CCDSiCCDS5789.1.
RefSeqiNP_036401.2. NM_012269.2.
XP_011514292.1. XM_011515990.2.
XP_016867400.1. XM_017011911.1.
UniGeneiHs.28673.

3D structure databases

ProteinModelPortaliQ2M3T9.
SMRiQ2M3T9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117097. 3 interactors.
STRINGi9606.ENSP00000223026.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

PTM databases

iPTMnetiQ2M3T9.
PhosphoSitePlusiQ2M3T9.

Polymorphism and mutation databases

BioMutaiHYAL4.
DMDMi158564281.

Proteomic databases

PaxDbiQ2M3T9.
PeptideAtlasiQ2M3T9.
PRIDEiQ2M3T9.

Protocols and materials databases

DNASUi23553.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223026; ENSP00000223026; ENSG00000106302.
ENST00000476325; ENSP00000417186; ENSG00000106302.
GeneIDi23553.
KEGGihsa:23553.
UCSCiuc003vlc.4. human.

Organism-specific databases

CTDi23553.
DisGeNETi23553.
GeneCardsiHYAL4.
H-InvDBHIX0033519.
HGNCiHGNC:5323. HYAL4.
HPAiHPA029453.
MIMi604510. gene.
neXtProtiNX_Q2M3T9.
OpenTargetsiENSG00000106302.
PharmGKBiPA29574.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IECJ. Eukaryota.
ENOG410XPZT. LUCA.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ2M3T9.
KOiK01197.
OMAiSEDGEFT.
OrthoDBiEOG091G064G.
PhylomeDBiQ2M3T9.
TreeFamiTF321598.

Enzyme and pathway databases

BioCyciMetaCyc:HS02883-MONOMER.
ZFISH:HS02883-MONOMER.
BRENDAi3.2.1.35. 2681.

Miscellaneous databases

GenomeRNAii23553.
PROiQ2M3T9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000106302.
CleanExiHS_HYAL4.
ExpressionAtlasiQ2M3T9. baseline and differential.
GenevisibleiQ2M3T9. HS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYAL4_HUMAN
AccessioniPrimary (citable) accession number: Q2M3T9
Secondary accession number(s): D0VXG1, Q9UL99, Q9Y6T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.