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Protein

Protein Shroom1

Gene

SHROOM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the assembly of microtubule arrays during cell elongation.By similarity

GO - Molecular functioni

  • actin filament binding Source: UniProtKB

GO - Biological processi

  • actin filament bundle assembly Source: UniProtKB
  • cell morphogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Shroom1
Alternative name(s):
Apical protein 2
Gene namesi
Name:SHROOM1
Synonyms:APXL2, KIAA1960
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:24084. SHROOM1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA147357345.

Polymorphism and mutation databases

BioMutaiSHROOM1.
DMDMi121941488.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 852852Protein Shroom1PRO_0000286061Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei103 – 1031PhosphothreonineCombined sources
Modified residuei133 – 1331PhosphoserineCombined sources
Modified residuei137 – 1371PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ2M3G4.
MaxQBiQ2M3G4.
PaxDbiQ2M3G4.
PRIDEiQ2M3G4.

PTM databases

iPTMnetiQ2M3G4.

Expressioni

Gene expression databases

BgeeiQ2M3G4.
CleanExiHS_SHROOM1.
ExpressionAtlasiQ2M3G4. baseline and differential.
GenevisibleiQ2M3G4. HS.

Organism-specific databases

HPAiHPA037690.
HPA037691.

Interactioni

Subunit structurei

Interacts with F-actin.By similarity

GO - Molecular functioni

  • actin filament binding Source: UniProtKB

Protein-protein interaction databases

BioGridi126408. 3 interactions.
DIPiDIP-42367N.
IntActiQ2M3G4. 5 interactions.
MINTiMINT-3974761.
STRINGi9606.ENSP00000367950.

Structurei

3D structure databases

ProteinModelPortaliQ2M3G4.
SMRiQ2M3G4. Positions 645-822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini145 – 23389ASD1PROSITE-ProRule annotationAdd
BLAST
Domaini543 – 825283ASD2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi713 – 7197Poly-Leu

Domaini

The ASD1 domain mediates F-actin binding.By similarity

Sequence similaritiesi

Belongs to the shroom family.Curated
Contains 1 ASD1 domain.PROSITE-ProRule annotation
Contains 1 ASD2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH4T. Eukaryota.
ENOG4111N2C. LUCA.
GeneTreeiENSGT00530000063061.
HOGENOMiHOG000293372.
HOVERGENiHBG105767.
InParanoidiQ2M3G4.
KOiK18625.
OMAiSFQRKEL.
OrthoDBiEOG754HNS.
PhylomeDBiQ2M3G4.
TreeFamiTF335754.

Family and domain databases

InterProiIPR014800. ASD1_dom.
IPR014799. ASD2_dom.
IPR027685. Shroom_fam.
[Graphical view]
PANTHERiPTHR15012. PTHR15012. 1 hit.
PfamiPF08688. ASD1. 1 hit.
PF08687. ASD2. 1 hit.
[Graphical view]
PROSITEiPS51306. ASD1. 1 hit.
PS51307. ASD2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2M3G4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEALGPGGDR ASPASSTSSL DLWHLSMRAD SAYSSFSAAS GGPEPRTQSP
60 70 80 90 100
GTDLLPYLDW DYVRVVWGGP GPAPPDAALC TSPRPRPAVA ARSGPQPTEV
110 120 130 140 150
PGTPGPLNRQ ATPLLYALAA EAEAAAQAAE PPSPPASRAA YRQRLQGAQR
160 170 180 190 200
RVLRETSFQR KELRMSLPAR LRPTVPARPP ATHPRSASLS HPGGEGEPAR
210 220 230 240 250
SRAPAPGTAG RGPLANQQRK WCFSEPGKLD RVGRGGGPAR ECLGEACSSS
260 270 280 290 300
GLPGPEPLEF QHPALAKFED HEVGWLPETQ PQGSMNLDSG SLKLGDAFRP
310 320 330 340 350
ASRSRSASGE VLGSWGGSGG TIPIVQAVPQ GAETPRPLFQ TKLSRFLPQK
360 370 380 390 400
EAAVMYPAEL PQSSPADSEQ RVSETCIVPA WLPSLPDEVF LEEAPLVRMR
410 420 430 440 450
SPPDPHASQG PPASVHASDQ PYGTGLGQRT GQVTVPTEYP LHECPGTAGA
460 470 480 490 500
DDCWQGVNGS VGISRPTSHT PTGTANDNIP TIDPTGLTTN PPTAAESDLL
510 520 530 540 550
KPVPADALGL SGNDTPGPSH NTALARGTGQ PGSRPTWPSQ CLEELVQELA
560 570 580 590 600
RLDPSLCDPL ASQPSPEPPL GLLDGLIPLA EVRAAMRPAC GEAGEEAAST
610 620 630 640 650
FEPGSYQFSF TQLLPAPREE TRLENPATHP VLDQPCGQGL PAPNNSIQGK
660 670 680 690 700
KVELAARLQK MLQDLHTEQE RLQGEAQAWA RRQAALEAAV RQACAPQELE
710 720 730 740 750
RFSRFMADLE RVLGLLLLLG SRLARVRRAL ARAASDSDPD EQASLLQRLR
760 770 780 790 800
LLQRQEEDAK ELKEHVARRE RAVREVLVRA LPVEELRVYC ALLAGKAAVL
810 820 830 840 850
AQQRNLDERI RLLQDQLDAI RDDLGHHAPS PSPARPPGTC PPVQPPFPLL

LT
Length:852
Mass (Da):90,786
Last modified:February 21, 2006 - v1
Checksum:i43DFAF54E8355877
GO
Isoform 2 (identifier: Q2M3G4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     742-746: Missing.

Show »
Length:847
Mass (Da):90,274
Checksum:iB41E6BB4B11EF6D0
GO

Sequence cautioni

The sequence BAB85546.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291A → V in AAM15526 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti180 – 1801P → L.
Corresponds to variant rs2292030 [ dbSNP | Ensembl ].
VAR_032061

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei742 – 7465Missing in isoform 2. 1 PublicationVSP_024962

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314142 mRNA. Translation: AAM15526.1.
AB075840 mRNA. Translation: BAB85546.1. Different initiation.
AC004775 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW62311.1.
CH471062 Genomic DNA. Translation: EAW62312.1.
BC104914 mRNA. Translation: AAI04915.1.
BC104916 mRNA. Translation: AAI04917.1.
BC143494 mRNA. Translation: AAI43495.1.
CCDSiCCDS4161.1. [Q2M3G4-2]
CCDS54902.1. [Q2M3G4-1]
RefSeqiNP_001166171.1. NM_001172700.1. [Q2M3G4-1]
NP_597713.2. NM_133456.2. [Q2M3G4-2]
XP_005271942.1. XM_005271885.3. [Q2M3G4-1]
XP_005271943.1. XM_005271886.3. [Q2M3G4-1]
XP_005271944.1. XM_005271887.3. [Q2M3G4-1]
XP_011541468.1. XM_011543166.1. [Q2M3G4-1]
XP_011541469.1. XM_011543167.1. [Q2M3G4-1]
UniGeneiHs.519574.

Genome annotation databases

EnsembliENST00000319854; ENSP00000324245; ENSG00000164403. [Q2M3G4-2]
ENST00000378679; ENSP00000367950; ENSG00000164403. [Q2M3G4-1]
ENST00000617339; ENSP00000478436; ENSG00000164403. [Q2M3G4-1]
GeneIDi134549.
KEGGihsa:134549.
UCSCiuc003kxx.3. human. [Q2M3G4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314142 mRNA. Translation: AAM15526.1.
AB075840 mRNA. Translation: BAB85546.1. Different initiation.
AC004775 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW62311.1.
CH471062 Genomic DNA. Translation: EAW62312.1.
BC104914 mRNA. Translation: AAI04915.1.
BC104916 mRNA. Translation: AAI04917.1.
BC143494 mRNA. Translation: AAI43495.1.
CCDSiCCDS4161.1. [Q2M3G4-2]
CCDS54902.1. [Q2M3G4-1]
RefSeqiNP_001166171.1. NM_001172700.1. [Q2M3G4-1]
NP_597713.2. NM_133456.2. [Q2M3G4-2]
XP_005271942.1. XM_005271885.3. [Q2M3G4-1]
XP_005271943.1. XM_005271886.3. [Q2M3G4-1]
XP_005271944.1. XM_005271887.3. [Q2M3G4-1]
XP_011541468.1. XM_011543166.1. [Q2M3G4-1]
XP_011541469.1. XM_011543167.1. [Q2M3G4-1]
UniGeneiHs.519574.

3D structure databases

ProteinModelPortaliQ2M3G4.
SMRiQ2M3G4. Positions 645-822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126408. 3 interactions.
DIPiDIP-42367N.
IntActiQ2M3G4. 5 interactions.
MINTiMINT-3974761.
STRINGi9606.ENSP00000367950.

PTM databases

iPTMnetiQ2M3G4.

Polymorphism and mutation databases

BioMutaiSHROOM1.
DMDMi121941488.

Proteomic databases

EPDiQ2M3G4.
MaxQBiQ2M3G4.
PaxDbiQ2M3G4.
PRIDEiQ2M3G4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319854; ENSP00000324245; ENSG00000164403. [Q2M3G4-2]
ENST00000378679; ENSP00000367950; ENSG00000164403. [Q2M3G4-1]
ENST00000617339; ENSP00000478436; ENSG00000164403. [Q2M3G4-1]
GeneIDi134549.
KEGGihsa:134549.
UCSCiuc003kxx.3. human. [Q2M3G4-1]

Organism-specific databases

CTDi134549.
GeneCardsiSHROOM1.
HGNCiHGNC:24084. SHROOM1.
HPAiHPA037690.
HPA037691.
MIMi611179. gene.
neXtProtiNX_Q2M3G4.
PharmGKBiPA147357345.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IH4T. Eukaryota.
ENOG4111N2C. LUCA.
GeneTreeiENSGT00530000063061.
HOGENOMiHOG000293372.
HOVERGENiHBG105767.
InParanoidiQ2M3G4.
KOiK18625.
OMAiSFQRKEL.
OrthoDBiEOG754HNS.
PhylomeDBiQ2M3G4.
TreeFamiTF335754.

Miscellaneous databases

GenomeRNAii134549.
PROiQ2M3G4.
SOURCEiSearch...

Gene expression databases

BgeeiQ2M3G4.
CleanExiHS_SHROOM1.
ExpressionAtlasiQ2M3G4. baseline and differential.
GenevisibleiQ2M3G4. HS.

Family and domain databases

InterProiIPR014800. ASD1_dom.
IPR014799. ASD2_dom.
IPR027685. Shroom_fam.
[Graphical view]
PANTHERiPTHR15012. PTHR15012. 1 hit.
PfamiPF08688. ASD1. 1 hit.
PF08687. ASD2. 1 hit.
[Graphical view]
PROSITEiPS51306. ASD1. 1 hit.
PS51307. ASD2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cytoplasmic tail of the melanoma cell adhesion molecule MCAM mediates selective binding of APXL2, a new member of the Apx/Shroom actin-binding protein family."
    Karlen S., Staub O., Rohrbach B., Braathen L.R.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins."
    Nagase T., Kikuno R., Ohara O.
    DNA Res. 8:319-327(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.
  6. Cited for: NOMENCLATURE.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-103; SER-133 AND SER-137, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSHRM1_HUMAN
AccessioniPrimary (citable) accession number: Q2M3G4
Secondary accession number(s): B7WP40
, B7ZL01, Q8TDP0, Q8TF41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 21, 2006
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.