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Protein

A-kinase anchor protein SPHKAP

Gene

SPHKAP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.2 Publications

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein SPHKAP
Alternative name(s):
SPHK1-interactor and AKAP domain-containing protein
Sphingosine kinase type 1-interacting protein
Gene namesi
Name:SPHKAP
Synonyms:KIAA1678, SKIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:30619. SPHKAP.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • mitochondrion Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162404477.

Polymorphism and mutation databases

BioMutaiSPHKAP.
DMDMi121941477.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17001700A-kinase anchor protein SPHKAPPRO_0000320666Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1025 – 10251PhosphoserineBy similarity
Modified residuei1085 – 10851PhosphoserineBy similarity
Modified residuei1107 – 11071PhosphoserineBy similarity
Modified residuei1120 – 11201PhosphoserineBy similarity
Modified residuei1121 – 11211PhosphoserineBy similarity
Modified residuei1124 – 11241PhosphoserineBy similarity
Modified residuei1259 – 12591PhosphoserineBy similarity
Modified residuei1288 – 12881PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2M3C7.
PeptideAtlasiQ2M3C7.
PRIDEiQ2M3C7.

PTM databases

iPTMnetiQ2M3C7.
PhosphoSiteiQ2M3C7.

Expressioni

Tissue specificityi

Highly expressed in heart. Both isoforms abundantly expressed in ventricle. Also expressed in spleen, ovary and brain.2 Publications

Gene expression databases

BgeeiENSG00000153820.
CleanExiHS_SPHKAP.
GenevisibleiQ2M3C7. HS.

Organism-specific databases

HPAiHPA042499.
HPA048168.

Interactioni

Subunit structurei

Interacts (via the PKA-RII subunit binding domain) with the RI subunit of PKA. Interacts with SPHK1; the interaction greatly reduces SPHK1 activity.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SPHK1Q9NYA14EBI-1803914,EBI-985303

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

Protein-protein interaction databases

BioGridi123222. 1 interaction.
IntActiQ2M3C7. 1 interaction.
STRINGi9606.ENSP00000375909.

Structurei

3D structure databases

ProteinModelPortaliQ2M3C7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni929 – 94618PKA-RII subunit binding domainBy similarityAdd
BLAST

Domaini

RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.By similarity

Sequence similaritiesi

Belongs to the AKAP110 family.Curated

Phylogenomic databases

eggNOGiENOG410IHCW. Eukaryota.
ENOG41113DA. LUCA.
GeneTreeiENSGT00530000063606.
HOGENOMiHOG000154389.
HOVERGENiHBG108508.
InParanoidiQ2M3C7.
OMAiYYAGKNA.
OrthoDBiEOG091G00G3.
PhylomeDBiQ2M3C7.
TreeFamiTF105426.

Family and domain databases

InterProiIPR018292. AKAP_110_C.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2M3C7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGNSLLSVP SNLESSRMYD VLEPQQGRGC GSSGSGPGNS ITACKKVLRS
60 70 80 90 100
NSLLESTDYW LQNQRMPCQI GFVEDKSENC ASVCFVNLDV NKDECSTEHL
110 120 130 140 150
QQKLVNVSPD LPKLISSMNV QQPKENEIVV LSGLASGNLQ ADFEVSQCPW
160 170 180 190 200
LPDICLVQCA RGNRPNSTNC IIFEINKFLI GLELVQERQL HLETNILKLE
210 220 230 240 250
DDTNCSLSSI EEDFLTASEH LEEESEVDES RNDYENINVS ANVLESKQLK
260 270 280 290 300
GATQVEWNCN KEKWLYALED KYINKYPTPL IKTERSPENL TKNTALQSLD
310 320 330 340 350
PSAKPSQWKR EAVGNGRQAT HYYHSEAFKG QMEKSQALYI PKDAYFSMMD
360 370 380 390 400
KDVPSACAVA EQRSNLNPGD HEDTRNALPP RQDGEVTTGK YATNLAESVL
410 420 430 440 450
QDAFIRLSQS QSTLPQESAV SVSVGSSLLP SCYSTKDTVV SRSWNELPKI
460 470 480 490 500
VVVQSPDGSD AAPQPGISSW PEMEVSVETS SILSGENSSR QPQSALEVAL
510 520 530 540 550
ACAATVIGTI SSPQATERLK MEQVVSNFPP GSSGALQTQA PQGLKEPSIN
560 570 580 590 600
EYSFPSALCG MTQVASAVAV CGLGEREEVT CSVAPSGSLP PAAEASEAMP
610 620 630 640 650
PLCGLASMEL GKEAIAKGLL KEAALVLTRP NTYSSIGDFL DSMNRRIMET
660 670 680 690 700
ASKSQTLCSE NVVRNELAHT LSNVILRHSI DEVHHKNMII DPNDNRHSSE
710 720 730 740 750
ILDTLMESTN QLLLDVICFT FKKMSHIVRL GECPAVLSKE TIRRRETEPS
760 770 780 790 800
CQPSDPGASQ AWTKATESSS SSPLSNSHNT SLVINNLVDG MYSKQDKGGV
810 820 830 840 850
RPGLFKNPTL QSQLSRSHRV PDSSTATTSS KEIYLKGIAG EDTKSPHHSE
860 870 880 890 900
NECRASSEGQ RSPTVSQSRS GSQEAEESIH PNTQEKYNCA TSRINEVQVN
910 920 930 940 950
LSLLGDDLLL PAQSTLQTKH PDIYCITDFA EELADTVVSM ATEIAAICLD
960 970 980 990 1000
NSSGKQPWFC AWKRGSEFLM TPNVPCRSLK RKKESQGSGT AVRKHKPPRL
1010 1020 1030 1040 1050
SEIKRKTDEH PELKEKLMNR VVDESMNLED VPDSVNLFAN EVAAKIMNLT
1060 1070 1080 1090 1100
EFSMVDGMWQ AQGYPRNRLL SGDRWSRLKA SSCESIPEED SEARAYVNSL
1110 1120 1130 1140 1150
GLMSTLSQPV SRASSVSKQS SCESITDEFS RFMVNQMENE GRGFELLLDY
1160 1170 1180 1190 1200
YAGKNASSIL NSAMQQACRK SDHLSVRPSC PSKQSSTESI TEEFYRYMLR
1210 1220 1230 1240 1250
DIERDSRESA SSRRSSQDWT AGLLSPSLRS PVCHRQSSMP DSRSPCSRLT
1260 1270 1280 1290 1300
VNVPIKANSL DGFAQNCPQD FLSVQPVSSA SSSGLCKSDS CLYRRGGTDH
1310 1320 1330 1340 1350
ITNMLIHETW ASSIEALMRK NKIIVDDAEE ADTEPVSGGS PSQAEKCANR
1360 1370 1380 1390 1400
LAASRMCSGP TLLVQESLDC PRKDSVTECK QPPVSSLSKT ASLTNHSPLD
1410 1420 1430 1440 1450
SKKETSSCQD PVPINHKRRS LCSREVPLIQ IETDQREACA GEPEPFLSKS
1460 1470 1480 1490 1500
SLLEEAEGHS NDKNIPDVVR GGDTAVSACQ IHSDSLDTRD VPEAEASTEA
1510 1520 1530 1540 1550
RAPDEAPNPP SSSEESTGSW TQLANEEDNP DDTSSFLQLS ERSMSNGNSS
1560 1570 1580 1590 1600
ATSSLGIMDL DIYQESMPSS PMINELVEEK KILKGQSEST EAPASGPPTG
1610 1620 1630 1640 1650
TASPQRSLLV INFDLEPECP DAELRATLQW IAASELGIPT IYFKKSQENR
1660 1670 1680 1690 1700
IEKFLDVVQL VHRKSWKVGD IFHAVVQYCK MHEEQKDGRL SLFDWLLELG
Length:1,700
Mass (Da):186,456
Last modified:February 21, 2006 - v1
Checksum:i3D49589CAC5A1BDE
GO
Isoform 2 (identifier: Q2M3C7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1546-1574: Missing.

Show »
Length:1,671
Mass (Da):183,414
Checksum:iFB6CD08DE8CC4797
GO

Sequence cautioni

The sequence CAH18152 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891D → G in CAH18317 (PubMed:17974005).Curated
Sequence conflicti483 – 4831L → P in CAH18317 (PubMed:17974005).Curated
Sequence conflicti719 – 7191F → S in CAH18152 (PubMed:17974005).Curated
Sequence conflicti921 – 9211P → L in CAH18152 (PubMed:17974005).Curated
Sequence conflicti1218 – 12181D → I in CAH18317 (PubMed:17974005).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti425 – 4251G → R.
Corresponds to variant rs4283414 [ dbSNP | Ensembl ].
VAR_039263
Natural varianti617 – 6171K → E.2 Publications
Corresponds to variant rs3811514 [ dbSNP | Ensembl ].
VAR_039264
Natural varianti847 – 8471H → Q.2 Publications
Corresponds to variant rs3811515 [ dbSNP | Ensembl ].
VAR_039265
Natural varianti867 – 8671Q → R.
Corresponds to variant rs3828161 [ dbSNP | Ensembl ].
VAR_039266
Natural varianti1603 – 16031S → R.
Corresponds to variant rs16824283 [ dbSNP | Ensembl ].
VAR_059113

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1546 – 157429Missing in isoform 2. 2 PublicationsVSP_031712Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR749297 mRNA. Translation: CAH18152.1. Different initiation.
CR749494 mRNA. Translation: CAH18317.1.
BC104954 mRNA. Translation: AAI04955.1.
BC104956 mRNA. Translation: AAI04957.1.
AB051465 mRNA. Translation: BAB21769.1.
CCDSiCCDS33389.1. [Q2M3C7-2]
CCDS46537.1. [Q2M3C7-1]
RefSeqiNP_001136116.1. NM_001142644.1. [Q2M3C7-1]
NP_085126.2. NM_030623.3. [Q2M3C7-2]
UniGeneiHs.436306.

Genome annotation databases

EnsembliENST00000344657; ENSP00000339886; ENSG00000153820. [Q2M3C7-2]
ENST00000392056; ENSP00000375909; ENSG00000153820. [Q2M3C7-1]
GeneIDi80309.
KEGGihsa:80309.
UCSCiuc002vpp.3. human. [Q2M3C7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR749297 mRNA. Translation: CAH18152.1. Different initiation.
CR749494 mRNA. Translation: CAH18317.1.
BC104954 mRNA. Translation: AAI04955.1.
BC104956 mRNA. Translation: AAI04957.1.
AB051465 mRNA. Translation: BAB21769.1.
CCDSiCCDS33389.1. [Q2M3C7-2]
CCDS46537.1. [Q2M3C7-1]
RefSeqiNP_001136116.1. NM_001142644.1. [Q2M3C7-1]
NP_085126.2. NM_030623.3. [Q2M3C7-2]
UniGeneiHs.436306.

3D structure databases

ProteinModelPortaliQ2M3C7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123222. 1 interaction.
IntActiQ2M3C7. 1 interaction.
STRINGi9606.ENSP00000375909.

PTM databases

iPTMnetiQ2M3C7.
PhosphoSiteiQ2M3C7.

Polymorphism and mutation databases

BioMutaiSPHKAP.
DMDMi121941477.

Proteomic databases

PaxDbiQ2M3C7.
PeptideAtlasiQ2M3C7.
PRIDEiQ2M3C7.

Protocols and materials databases

DNASUi80309.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344657; ENSP00000339886; ENSG00000153820. [Q2M3C7-2]
ENST00000392056; ENSP00000375909; ENSG00000153820. [Q2M3C7-1]
GeneIDi80309.
KEGGihsa:80309.
UCSCiuc002vpp.3. human. [Q2M3C7-1]

Organism-specific databases

CTDi80309.
GeneCardsiSPHKAP.
H-InvDBHIX0161878.
HGNCiHGNC:30619. SPHKAP.
HPAiHPA042499.
HPA048168.
MIMi611646. gene.
neXtProtiNX_Q2M3C7.
PharmGKBiPA162404477.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IHCW. Eukaryota.
ENOG41113DA. LUCA.
GeneTreeiENSGT00530000063606.
HOGENOMiHOG000154389.
HOVERGENiHBG108508.
InParanoidiQ2M3C7.
OMAiYYAGKNA.
OrthoDBiEOG091G00G3.
PhylomeDBiQ2M3C7.
TreeFamiTF105426.

Miscellaneous databases

GenomeRNAii80309.
PROiQ2M3C7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000153820.
CleanExiHS_SPHKAP.
GenevisibleiQ2M3C7. HS.

Family and domain databases

InterProiIPR018292. AKAP_110_C.
IPR008382. SPHK1-interactor_AKAP_110.
[Graphical view]
PANTHERiPTHR10226. PTHR10226. 1 hit.
PfamiPF05716. AKAP_110. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPKAP_HUMAN
AccessioniPrimary (citable) accession number: Q2M3C7
Secondary accession number(s): Q68DA3, Q68DR8, Q9C0I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 21, 2006
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.