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Protein

Peroxisomal targeting signal 1 receptor

Gene

Pex5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal targeting signal 1 receptor
Short name:
PTS1 receptor
Short name:
PTS1R
Alternative name(s):
PTS1-BP
Peroxin-5
Peroxisomal C-terminal targeting signal import receptor
Peroxisome receptor 1
Gene namesi
Name:Pex5
Synonyms:Pex5_predicted
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1307612. Pex5.

Subcellular locationi

  • Cytoplasm By similarity
  • Peroxisome membrane By similarity; Peripheral membrane protein By similarity

  • Note: Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor (PEX13) (By similarity).By similarity

GO - Cellular componenti

  • cytosol Source: RGD
  • membrane Source: RGD
  • peroxisomal membrane Source: HGNC
  • peroxisome Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 640640Peroxisomal targeting signal 1 receptorPRO_0000396503Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki11 – 11Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei114 – 1141PhosphoserineBy similarity
Modified residuei152 – 1521PhosphoserineBy similarity
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei166 – 1661PhosphoserineBy similarity
Modified residuei280 – 2801PhosphoserineBy similarity

Post-translational modificationi

Monoubiquitination at Cys-11 is required for proper export from peroxisomes and recycling.By similarity

Keywords - PTMi

Phosphoprotein, Thioester bond, Ubl conjugation

Proteomic databases

PaxDbiQ2M2R8.

PTM databases

iPTMnetiQ2M2R8.

Expressioni

Gene expression databases

GenevisibleiQ2M2R8. RN.

Interactioni

Subunit structurei

Interacts with PEX7, PEX12, PEX13 and PEX14.By similarity

Protein-protein interaction databases

BioGridi260235. 4 interactions.
STRINGi10116.ENSRNOP00000014028.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati336 – 36934TPR 1Add
BLAST
Repeati371 – 40333TPR 2Add
BLAST
Repeati404 – 43734TPR 3Add
BLAST
Repeati454 – 48633TPR 4Add
BLAST
Repeati489 – 52234TPR 5Add
BLAST
Repeati524 – 55633TPR 6Add
BLAST
Repeati558 – 59033TPR 7Add
BLAST

Sequence similaritiesi

Contains 7 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1125. Eukaryota.
ENOG410XQ6Q. LUCA.
GeneTreeiENSGT00390000013941.
HOGENOMiHOG000158146.
HOVERGENiHBG053575.
InParanoidiQ2M2R8.
KOiK13342.
OMAiETNESMQ.
PhylomeDBiQ2M2R8.
TreeFamiTF315044.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024111. PTS1R_family.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10130. PTHR10130. 1 hit.
PfamiPF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2M2R8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMRELVESE CGGANPLMKL ATHFTQDKAL RQEGLRPPWP PGASAAETVS
60 70 80 90 100
KPLGVGTEDE LVAEFLQDQN ATLVSRAPQT FKMDDLLAEM QEIEQSNFRQ
110 120 130 140 150
APQRAPGVAD LALSENWAQE FLAAGDAVDV AQDYNETDWS QEFIAEVTDP
160 170 180 190 200
LSVSPARWAE EYLEQSEEKL WLGDPEGTST TDRWYDEYHP EEDLQHTASD
210 220 230 240 250
FVSKVDDPKL ANSEFLKFVR QIGEGQVSLE SAAGSGRAQA EQWAAEFIQQ
260 270 280 290 300
QGTSEAWVDQ FTRPGNKLAA LQVEFERAKS AIESDVDFWD KLQAELEEMA
310 320 330 340 350
KRDAEAHPWL SDYDDLTSAS YDKGYQFEEE NPLRDHPQPF EEGLRRLEEG
360 370 380 390 400
DLPNAVLLFE AAVQQDPKHM EAWQYLGTTQ AENEQELLAI SALRRCLELK
410 420 430 440 450
PDNRTALMAL AVSFTNESLQ RQACETLRDW LRYSPAYAHL VAPGEEGASG
460 470 480 490 500
AGLGPSKRVL GSLLSDSLFL EVKELFLAAV RLDPTSIDPD VQCGLGVLFN
510 520 530 540 550
LSGEYDKAVD CFTAALSVRP NDYLLWNKLG ATLANGNQSE EAVAAYRRAL
560 570 580 590 600
ELQPGYIRSR YNLGISCINL GAHREAVEHF LEALNMQRKS RGPRGEGGAM
610 620 630 640
SENIWSTLRL ALSMLGQSDA YGAADARDLP ALLTMFGLPQ
Length:640
Mass (Da):71,022
Last modified:August 10, 2010 - v2
Checksum:i57B65CDA460A5BD6
GO

Sequence cautioni

The sequence EDM01958.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473964 Genomic DNA. Translation: EDM01958.1. Sequence problems.
BC111709 mRNA. Translation: AAI11710.1.
RefSeqiNP_001164055.1. NM_001170584.1.
XP_006237446.1. XM_006237384.2.
XP_006237447.1. XM_006237385.2.
XP_008761499.1. XM_008763277.1.
UniGeneiRn.14316.

Genome annotation databases

EnsembliENSRNOT00000085675; ENSRNOP00000070292; ENSRNOG00000010407.
GeneIDi103690024.
312703.
KEGGirno:103690024.
rno:312703.
UCSCiRGD:1307612. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473964 Genomic DNA. Translation: EDM01958.1. Sequence problems.
BC111709 mRNA. Translation: AAI11710.1.
RefSeqiNP_001164055.1. NM_001170584.1.
XP_006237446.1. XM_006237384.2.
XP_006237447.1. XM_006237385.2.
XP_008761499.1. XM_008763277.1.
UniGeneiRn.14316.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi260235. 4 interactions.
STRINGi10116.ENSRNOP00000014028.

PTM databases

iPTMnetiQ2M2R8.

Proteomic databases

PaxDbiQ2M2R8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000085675; ENSRNOP00000070292; ENSRNOG00000010407.
GeneIDi103690024.
312703.
KEGGirno:103690024.
rno:312703.
UCSCiRGD:1307612. rat.

Organism-specific databases

CTDi5830.
RGDi1307612. Pex5.

Phylogenomic databases

eggNOGiKOG1125. Eukaryota.
ENOG410XQ6Q. LUCA.
GeneTreeiENSGT00390000013941.
HOGENOMiHOG000158146.
HOVERGENiHBG053575.
InParanoidiQ2M2R8.
KOiK13342.
OMAiETNESMQ.
PhylomeDBiQ2M2R8.
TreeFamiTF315044.

Miscellaneous databases

PROiQ2M2R8.

Gene expression databases

GenevisibleiQ2M2R8. RN.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR024111. PTS1R_family.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR10130. PTHR10130. 1 hit.
PfamiPF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 5 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 408-604.
    Tissue: Liver.
  3. "Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor."
    Grou C.P., Carvalho A.F., Pinto M.P., Wiese S., Piechura H., Meyer H.E., Warscheid B., Sa-Miranda C., Azevedo J.E.
    J. Biol. Chem. 283:14190-14197(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION AT CYS-11.

Entry informationi

Entry nameiPEX5_RAT
AccessioniPrimary (citable) accession number: Q2M2R8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: May 11, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.