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Protein

Speckle-type POZ protein-like

Gene

Spopl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle-type POZ protein-like
Alternative name(s):
HIB homolog 2
Gene namesi
Name:Spopl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924107. Spopl.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Speckle-type POZ protein-likePRO_0000274589Add
BLAST

Proteomic databases

PaxDbiQ2M2N2.
PRIDEiQ2M2N2.

Expressioni

Gene expression databases

BgeeiQ2M2N2.
CleanExiMM_SPOPL.
ExpressionAtlasiQ2M2N2. baseline and differential.
GenevisibleiQ2M2N2. MM.

Interactioni

Subunit structurei

Homodimer. Heterodimer with SPOP. Component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL. Interacts with CUL3 and H2AFY (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114974.

Structurei

3D structure databases

ProteinModelPortaliQ2M2N2.
SMRiQ2M2N2. Positions 28-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 161131MATHPROSITE-ProRule annotationAdd
BLAST
Domaini200 – 26768BTBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Tdpoz family.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 1 MATH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1987. Eukaryota.
ENOG410XQV8. LUCA.
GeneTreeiENSGT00390000000361.
HOGENOMiHOG000231621.
HOVERGENiHBG001393.
InParanoidiQ2M2N2.
OMAiSLMCESV.
OrthoDBiEOG73NG3G.
TreeFamiTF313419.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR002083. MATH/TRAF_dom.
IPR011333. SKP1/BTB/POZ.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2M2N2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSREPTPPLP GDMSTSPVAE SWCYTQVKVV KFSYMWTINN FSFCREEMGE
60 70 80 90 100
VLKSSTFSSG PNDKMKWCLR VNPKGLDDES KDYLSLYLLL VSCPKSEVRA
110 120 130 140 150
KFKFSLLNDK REETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL
160 170 180 190 200
PDDKLTLFCE VSVVQDSVNV SGHTSTNTLK VPECRLAEDL GNLWENTRFT
210 220 230 240 250
DCCFFVRGKE FKAHKSVLAA RSPVFNAMFE HEMEECTKNR VEINDLDPEV
260 270 280 290 300
FKEMMRFVYT GKAPNLDKMA DNLLAAADKY ALERLKVMCE EALCSNLSVE
310 320 330 340 350
NVADTLVLAD LHSAEQLKAQ AIDFINRCSV LRQLGCKDGK NWNNNQATDI
360 370 380 390
METSGWKSMI QSHPHLVAEA FRALASSQCP QFGIPRKRLK QS
Length:392
Mass (Da):44,699
Last modified:July 27, 2011 - v3
Checksum:i22471681536252CA
GO
Isoform 2 (identifier: Q2M2N2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-160: MSREPTPPLP...PDDKLTLFCE → MIKGKKQKQW...PLHCKWCCRQ

Show »
Length:317
Mass (Da):35,943
Checksum:i4449A1065781C7A3
GO

Sequence cautioni

The sequence AAI11868.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE36701.1 differs from that shown. Reason: Frameshift at position 162. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti202 – 2021C → G in BAE36701 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 160160MSREP…TLFCE → MIKGKKQKQWKAKEHIDLYR GRTGDLKNSLEGIFCLMKLM VSFQMTSLRYSVSISNTACR RGGSIALPSLKNWHLPLHCK WCCRQ in isoform 2. 2 PublicationsVSP_022824Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK162056 mRNA. Translation: BAE36701.1. Frameshift.
AL773534 Genomic DNA. Translation: CAM16793.1.
CH466542 Genomic DNA. Translation: EDL08155.1.
BC111867 mRNA. Translation: AAI11868.1. Different initiation.
CCDSiCCDS15730.1. [Q2M2N2-1]
RefSeqiNP_001159469.1. NM_001165997.1. [Q2M2N2-2]
NP_001159470.1. NM_001165998.1. [Q2M2N2-2]
NP_084049.2. NM_029773.2. [Q2M2N2-1]
XP_006498477.1. XM_006498414.1. [Q2M2N2-1]
XP_011237511.1. XM_011239209.1. [Q2M2N2-2]
UniGeneiMm.276415.
Mm.480695.

Genome annotation databases

EnsembliENSMUST00000132484; ENSMUSP00000114974; ENSMUSG00000026771. [Q2M2N2-1]
GeneIDi76857.
KEGGimmu:76857.
UCSCiuc008ioh.2. mouse. [Q2M2N2-1]
uc008ioi.2. mouse. [Q2M2N2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK162056 mRNA. Translation: BAE36701.1. Frameshift.
AL773534 Genomic DNA. Translation: CAM16793.1.
CH466542 Genomic DNA. Translation: EDL08155.1.
BC111867 mRNA. Translation: AAI11868.1. Different initiation.
CCDSiCCDS15730.1. [Q2M2N2-1]
RefSeqiNP_001159469.1. NM_001165997.1. [Q2M2N2-2]
NP_001159470.1. NM_001165998.1. [Q2M2N2-2]
NP_084049.2. NM_029773.2. [Q2M2N2-1]
XP_006498477.1. XM_006498414.1. [Q2M2N2-1]
XP_011237511.1. XM_011239209.1. [Q2M2N2-2]
UniGeneiMm.276415.
Mm.480695.

3D structure databases

ProteinModelPortaliQ2M2N2.
SMRiQ2M2N2. Positions 28-374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114974.

Proteomic databases

PaxDbiQ2M2N2.
PRIDEiQ2M2N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000132484; ENSMUSP00000114974; ENSMUSG00000026771. [Q2M2N2-1]
GeneIDi76857.
KEGGimmu:76857.
UCSCiuc008ioh.2. mouse. [Q2M2N2-1]
uc008ioi.2. mouse. [Q2M2N2-2]

Organism-specific databases

CTDi339745.
MGIiMGI:1924107. Spopl.

Phylogenomic databases

eggNOGiKOG1987. Eukaryota.
ENOG410XQV8. LUCA.
GeneTreeiENSGT00390000000361.
HOGENOMiHOG000231621.
HOVERGENiHBG001393.
InParanoidiQ2M2N2.
OMAiSLMCESV.
OrthoDBiEOG73NG3G.
TreeFamiTF313419.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiSpopl. mouse.
PROiQ2M2N2.
SOURCEiSearch...

Gene expression databases

BgeeiQ2M2N2.
CleanExiMM_SPOPL.
ExpressionAtlasiQ2M2N2. baseline and differential.
GenevisibleiQ2M2N2. MM.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR002083. MATH/TRAF_dom.
IPR011333. SKP1/BTB/POZ.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Ovary.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. "A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor."
    Zhang Q., Zhang L., Wang B., Ou C.-Y., Chien C.-T., Jiang J.
    Dev. Cell 10:719-729(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiSPOPL_MOUSE
AccessioniPrimary (citable) accession number: Q2M2N2
Secondary accession number(s): A2AL52, Q3TSH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.