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Protein

Speckle-type POZ protein-like

Gene

Spopl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5632684 Hedgehog 'on' state
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle-type POZ protein-like
Alternative name(s):
HIB homolog 2
Gene namesi
Name:Spopl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1924107 Spopl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745891 – 392Speckle-type POZ protein-likeAdd BLAST392

Proteomic databases

MaxQBiQ2M2N2
PaxDbiQ2M2N2
PRIDEiQ2M2N2

PTM databases

PhosphoSitePlusiQ2M2N2

Expressioni

Gene expression databases

BgeeiENSMUSG00000026771
CleanExiMM_SPOPL
ExpressionAtlasiQ2M2N2 baseline and differential
GenevisibleiQ2M2N2 MM

Interactioni

Subunit structurei

Homodimer. Heterodimer with SPOP. Component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL. Interacts with CUL3 and H2AFY (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114974

Structurei

3D structure databases

ProteinModelPortaliQ2M2N2
SMRiQ2M2N2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 161MATHPROSITE-ProRule annotationAdd BLAST131
Domaini200 – 267BTBPROSITE-ProRule annotationAdd BLAST68

Sequence similaritiesi

Belongs to the Tdpoz family.Curated

Phylogenomic databases

eggNOGiKOG1987 Eukaryota
ENOG410XQV8 LUCA
GeneTreeiENSGT00390000000361
HOGENOMiHOG000231621
HOVERGENiHBG001393
InParanoidiQ2M2N2
KOiK10523
OMAiMEESKKX
OrthoDBiEOG091G07ZM
TreeFamiTF313419

Family and domain databases

CDDicd14734 SPOP_C, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR002083 MATH/TRAF_dom
IPR011333 SKP1/BTB/POZ_sf
IPR034089 SPOP_C
IPR008974 TRAF-like
PfamiView protein in Pfam
PF00651 BTB, 1 hit
PF00917 MATH, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS50144 MATH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2M2N2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSREPTPPLP GDMSTSPVAE SWCYTQVKVV KFSYMWTINN FSFCREEMGE
60 70 80 90 100
VLKSSTFSSG PNDKMKWCLR VNPKGLDDES KDYLSLYLLL VSCPKSEVRA
110 120 130 140 150
KFKFSLLNDK REETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL
160 170 180 190 200
PDDKLTLFCE VSVVQDSVNV SGHTSTNTLK VPECRLAEDL GNLWENTRFT
210 220 230 240 250
DCCFFVRGKE FKAHKSVLAA RSPVFNAMFE HEMEECTKNR VEINDLDPEV
260 270 280 290 300
FKEMMRFVYT GKAPNLDKMA DNLLAAADKY ALERLKVMCE EALCSNLSVE
310 320 330 340 350
NVADTLVLAD LHSAEQLKAQ AIDFINRCSV LRQLGCKDGK NWNNNQATDI
360 370 380 390
METSGWKSMI QSHPHLVAEA FRALASSQCP QFGIPRKRLK QS
Length:392
Mass (Da):44,699
Last modified:July 27, 2011 - v3
Checksum:i22471681536252CA
GO
Isoform 2 (identifier: Q2M2N2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-160: MSREPTPPLP...PDDKLTLFCE → MIKGKKQKQW...PLHCKWCCRQ

Show »
Length:317
Mass (Da):35,943
Checksum:i4449A1065781C7A3
GO

Sequence cautioni

The sequence AAI11868 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE36701 differs from that shown. Reason: Frameshift at position 162.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202C → G in BAE36701 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0228241 – 160MSREP…TLFCE → MIKGKKQKQWKAKEHIDLYR GRTGDLKNSLEGIFCLMKLM VSFQMTSLRYSVSISNTACR RGGSIALPSLKNWHLPLHCK WCCRQ in isoform 2. 2 PublicationsAdd BLAST160

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK162056 mRNA Translation: BAE36701.1 Frameshift.
AL773534 Genomic DNA Translation: CAM16793.1
CH466542 Genomic DNA Translation: EDL08155.1
BC111867 mRNA Translation: AAI11868.1 Different initiation.
CCDSiCCDS15730.1 [Q2M2N2-1]
RefSeqiNP_001159469.1, NM_001165997.1 [Q2M2N2-2]
NP_001159470.1, NM_001165998.1 [Q2M2N2-2]
NP_084049.2, NM_029773.2 [Q2M2N2-1]
XP_006498477.1, XM_006498414.2 [Q2M2N2-1]
XP_017174813.1, XM_017319324.1 [Q2M2N2-2]
UniGeneiMm.276415
Mm.480695

Genome annotation databases

EnsembliENSMUST00000132484; ENSMUSP00000114974; ENSMUSG00000026771 [Q2M2N2-1]
GeneIDi76857
KEGGimmu:76857
UCSCiuc008ioh.2 mouse [Q2M2N2-1]
uc008ioi.2 mouse [Q2M2N2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSPOPL_MOUSE
AccessioniPrimary (citable) accession number: Q2M2N2
Secondary accession number(s): A2AL52, Q3TSH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 94 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health