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Protein

Probable maltase-glucoamylase 2

Gene

MGAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei478NucleophilePROSITE-ProRule annotation1
Active sitei481By similarity1
Active sitei1375NucleophilePROSITE-ProRule annotation1
Active sitei1378By similarity1

GO - Molecular functioni

GO - Biological processi

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable maltase-glucoamylase 2Curated
Alternative name(s):
Maltase-glucoamylase (alpha-glucosidase) pseudogene
Including the following 1 domains:
Glucoamylase (EC:3.2.1.3)
Alternative name(s):
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:MGAM2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000257743.8.
HGNCiHGNC:28101. MGAM2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Topological domaini31 – 482LumenalSequence analysisAdd BLAST452

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000257743.

Polymorphism and mutation databases

DMDMi190359876.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003413801 – 2515Probable maltase-glucoamylase 2Add BLAST2515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 72PROSITE-ProRule annotation
Disulfide bondi54 ↔ 71PROSITE-ProRule annotation
Disulfide bondi65 ↔ 84PROSITE-ProRule annotation
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei371SulfotyrosineSequence analysis1
Glycosylationi421N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi608 ↔ 619PROSITE-ProRule annotation
Glycosylationi613N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi916 ↔ 933PROSITE-ProRule annotation
Disulfide bondi928 ↔ 946PROSITE-ProRule annotation
Modified residuei1238SulfotyrosineSequence analysis1
Glycosylationi2249N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PeptideAtlasiQ2M2H8.
PRIDEiQ2M2H8.

PTM databases

iPTMnetiQ2M2H8.
PhosphoSitePlusiQ2M2H8.

Expressioni

Gene expression databases

BgeeiENSG00000257743.
GenevisibleiQ2M2H8. HS.

Organism-specific databases

HPAiHPA055697.

Structurei

3D structure databases

ProteinModelPortaliQ2M2H8.
SMRiQ2M2H8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 88P-type 1PROSITE-ProRule annotationAdd BLAST48
Domaini904 – 950P-type 2PROSITE-ProRule annotationAdd BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 865MaltaseBy similarityAdd BLAST714
Regioni1023 – 1766GlucoamylaseBy similarityAdd BLAST744

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 31Poly-Leu7
Compositional biasi979 – 983Poly-Ala5
Compositional biasi1789 – 2503Thr-richAdd BLAST715

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119229.
HOGENOMiHOG000067936.
HOVERGENiHBG095491.
KOiK12047.
OMAiTMPYPAS.
OrthoDBiEOG091G01E3.

Family and domain databases

CDDicd00111. Trefoil. 2 hits.
InterProiView protein in InterPro
IPR031727. Gal_mutarotase_N.
IPR011013. Gal_mutarotase_sf_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR017853. Glycoside_hydrolase_SF.
IPR000519. P_trefoil_dom.
PfamiView protein in Pfam
PF01055. Glyco_hydro_31. 2 hits.
PF16863. NtCtMGAM_N. 2 hits.
PF00088. Trefoil. 2 hits.
SMARTiView protein in SMART
SM00018. PD. 2 hits.
SUPFAMiSSF51445. SSF51445. 4 hits.
SSF74650. SSF74650. 2 hits.
PROSITEiView protein in PROSITE
PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00092. N6_MTASE. 1 hit.
PS51448. P_TREFOIL_2. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2M2H8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARKLSVLEV LLIIFCLIVV TIDILLLLLV LEETSDTSFT PECPEIPQSE
60 70 80 90 100
RIDCTPDQEV TEDICRWQYK CCWSPVADAN VPRCFFPWNW GYEASNGHTN
110 120 130 140 150
TSTGFTAQLK RLPSPSLFGN DVATTLFTAE YQTSNRFHFK ITDFNNIRYE
160 170 180 190 200
VSHENINLVD GIADASNLSY YVEVTDKPFS IKIMRTSNRR VLLDTSIGPL
210 220 230 240 250
QFAQQYLQLS FRLPSANVYG LGEHVHQQYR HNMTWKTWPI FTRDATPTEG
260 270 280 290 300
MINLYGAHTF FLCLEDARGS SFGVFLMNSN AMEVTLQPAP AITYRTIGGI
310 320 330 340 350
LDFYVFLGNT PEQVVQEYLE LVGRPFFPPY WSLGFQLSRR DYGGINKLKE
360 370 380 390 400
VVSRNRLAEI PYDVQYSDID YMDGKKDFTV DEVAYSGLPD FVKELHDNGQ
410 420 430 440 450
KYLIIMNPGI SKNSNYEPYN NGSLKRVWIL GSNGFAVGEG YPGPTVFPDY
460 470 480 490 500
TNPVCTEWWT DQVAKFHDHL EFDGVWIEMN EVSSLLQASN NQCESNNLNF
510 520 530 540 550
PPFLPRVLDH LLFARTLCMD TEFHGGLHYD IHSLYGHSMA RTTNLALETI
560 570 580 590 600
FMNNRSFILS RSTFAGSGKF AAHWLGDNAA TWDDLRWSIP TILEFNLFGI
610 620 630 640 650
PMVGANICGY NNNVTEELCR RWMQLGAFYP LPRNHNGPGF RDQDPAAFGV
660 670 680 690 700
DSLLLKSSRH YLNIRYTLLP YLYTLFYHAH TRGETVARPL VHEFYQDSAT
710 720 730 740 750
WDVHEQFLWG PGLLITPVLY EGVDEVKAYI PDATWYDYET GVAISWRKQL
760 770 780 790 800
VNMLLPGDKI GLHLRGGYIF PTQKPNTTTE ASRRNSLGLI IALDYKREAK
810 820 830 840 850
GELYWDDGVS KDAVTEKKYI LYDFSVTSNH LQAKIINNNY MDTDNLMFTD
860 870 880 890 900
ITILGMDKQP ANFIVLLNNV ATSSPSVVYN ASTKVVTITD LQGLVLGQEF
910 920 930 940 950
SIRWNLPVSD LEKFNCYPDD PTASEESCRQ RGCLWEDTST PGVPTCYYDT
960 970 980 990 1000
IPNYVASDIQ YLNTSITADL SLPMAPESAA AAASDSLSAK ISFLHLKVIY
1010 1020 1030 1040 1050
HTATMLQVKI YDPTNKRYEV PVPLNTPPQP VGDPENRLYD VRIQNNPFGI
1060 1070 1080 1090 1100
QIQRKNSSTV IWDSQLPGFI FNDMFLSIST RLPSQYIYGF GETEHTTFRR
1110 1120 1130 1140 1150
NMNWNTWGMF AHDEPPAYKK NSYGVHPYYM ALEEDGSAHG VLLLNSNAMD
1160 1170 1180 1190 1200
VTLQPTPALT YRTTGGILDF YIVLGPTPEL VTQQYTELIG RPAMIPYWAL
1210 1220 1230 1240 1250
GFHLSRYGYQ NDAEISSLYD AMVAAQIPYD VQHVDIDYMN RKLDFTLSAN
1260 1270 1280 1290 1300
FQNLSLLIEQ MKKNGMRFIL ILDPAISGNE TQYLPFIRGQ ENNVFIKWPD
1310 1320 1330 1340 1350
TNDIVWGKVW PDLPNVIVDG SLDHETQVKL YRAYVAFPDF FRNSTAAWWK
1360 1370 1380 1390 1400
KEIEELYANP REPEKSLKFD GLWIDMNEPS NFVDGSVRGC SNEMLNNPPY
1410 1420 1430 1440 1450
MPYLESRDKG LSSKTLCMES QQILPDSSPV EHYNVHNLYG WSQTRPTYEA
1460 1470 1480 1490 1500
VQEVTGQRGV IITRSTFPSS GRWGGHRLGN NTAAWDQLGK SIIGMMEFSL
1510 1520 1530 1540 1550
FGIPYTGADI CGFFGDAEYE MCVRWMQLGA FYPFSRNHNN IGTRRQDPVA
1560 1570 1580 1590 1600
WNSTFEMLSR KVLETRYTLL PYLYTLMHKA HVEGSTVVRP LLHEFTDDRT
1610 1620 1630 1640 1650
TWDIDRQFML GPAILISPVL ETSTFEISAY FPRARWYDYS TGTSSTSTGQ
1660 1670 1680 1690 1700
RKILKAPLDH INLHVRGGYI LPWQEPAMNT HSSRQNFMGL IVALDDNGTA
1710 1720 1730 1740 1750
EGQVFWDDGQ SIDTYENGNY FLANFIAAQN ILQIQTIHNK YLSDSNPLKV
1760 1770 1780 1790 1800
GYIRIWGVNT YVTQVSFTYD NRQFMETNFK SEPYNQILTI QLTDKTINLE
1810 1820 1830 1840 1850
KLTEVTWIDG GPVLPTPTKT STIPMSSHPS PSTTNATSSE TITSSASANT
1860 1870 1880 1890 1900
TTGTTDTVPI TTTSFPSTTS VTTNTTVPDT TSPFPTSTTN ASTNATVPIT
1910 1920 1930 1940 1950
TTPFPTSTIG VTTNATVPNT TAPFPTNAST ASTNATVPIT TTCFATSTIG
1960 1970 1980 1990 2000
VTTNATVPDT TAPFPTNTTT ASTNATIPIT TTPFATSTIS VTTSTTVPDT
2010 2020 2030 2040 2050
TAPFPTSTTS ASTNATPVPI TTTLFATSTI GVTTGTTVPD TTAPFPTSTT
2060 2070 2080 2090 2100
STSTSATVPI TTTPSPTNTA DANTSNTVPN TTMPSPTSST TVSTIATVPI
2110 2120 2130 2140 2150
SVTPSLTSTA DATISTTVLI ATTSSLTGTT DVSTSTTINN ISTPVQTNTT
2160 2170 2180 2190 2200
NASTSTNVAN ITATSHTSTD DTVPNNTVPV TAIPSLANTG VDTTSNSFSI
2210 2220 2230 2240 2250
MTTSFSESTN AMNTTVIMAT TSPTSTDVAS TNNDASMTNF LLATMSAGNI
2260 2270 2280 2290 2300
TSNSISITTT SFGNSVPFVT TPSPSTDATT TSNNTNPGMT TYYQTSPTIP
2310 2320 2330 2340 2350
THTLTSIPSS ITSILSMFPT SNTFTTDKIT NFTTPTNANT IIFNTLDTKS
2360 2370 2380 2390 2400
TMVIDATVTT TSTKDNTMSP DTTVTSIDKF TTHITQFATP HSATTTTLAL
2410 2420 2430 2440 2450
SHTSLAPTNL SNLGTMDITD ADNSSSVTGN TTHISVSNLT TASVTITATG
2460 2470 2480 2490 2500
LDSQTPHMVI NSVATYLPIT ATSATTDTTN ITKYALNTTT PDSTVHTSAT
2510
APTYIANAIN ATQVP
Note: No experimental confirmation available. Gene prediction based on EST data.
Length:2,515
Mass (Da):277,990
Last modified:September 3, 2014 - v3
Checksum:iF6E8F08987335A4F
GO
Isoform 2 (identifier: Q2M2H8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     478-482: EMNEV → VSYYS
     483-2515: Missing.

Show »
Length:482
Mass (Da):55,146
Checksum:i94EF4964B0B4E4AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti327F → L in BC111973 (PubMed:15489334).Curated1
Sequence conflicti327F → L in BC111975 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_055415478 – 482EMNEV → VSYYS in isoform 2. 1 Publication5
Alternative sequenceiVSP_055416483 – 2515Missing in isoform 2. 1 PublicationAdd BLAST2033

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC091742 Genomic DNA. No translation available.
CH236959 Genomic DNA. Translation: EAL23771.1.
CH471198 Genomic DNA. Translation: EAW51984.1.
BC111973 mRNA. No translation available.
BC111975 mRNA. No translation available.
RefSeqiNP_001280555.1. NM_001293626.1. [Q2M2H8-1]
UniGeneiHs.647098.

Genome annotation databases

EnsembliENST00000477922; ENSP00000420449; ENSG00000257743. [Q2M2H8-1]
ENST00000550469; ENSP00000447431; ENSG00000257743. [Q2M2H8-3]
ENST00000622623; ENSP00000484330; ENSG00000278563. [Q2M2H8-3]
ENST00000632823; ENSP00000488423; ENSG00000278563. [Q2M2H8-1]
GeneIDi93432.
KEGGihsa:93432.
UCSCiuc064isf.1. human. [Q2M2H8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMGAL_HUMAN
AccessioniPrimary (citable) accession number: Q2M2H8
Secondary accession number(s): A4D2I3, C9JNC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 3, 2014
Last modified: November 22, 2017
This is version 98 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although it belongs to the glycosyl hydrolase 31 family, it lacks one active site residue, thus suggesting it may lack some enzyme activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  3. SIMILARITY comments
    Index of protein domains and families