Ubiquitin carboxyl-terminal hydrolase 36 - Drosophila pseudoobscura pseudoobscura (Fruit fly)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 36
Protein scrawny
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36

Gene names

Name:	Usp36
Synonyms:	scny
ORF Names:	GA18934

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora ›

Protein attributes

Sequence length	1059 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with atms/PAF1, but not with CycT By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	germ-line stem cell maintenance Inferred from sequence or structural similarity. Source: UniProtKB protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB somatic stem cell maintenance Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleolus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1059

1059

Ubiquitin carboxyl-terminal hydrolase 36

PRO_0000378500

Sites

Active site

180

1

Nucleophile By similarity

Active site

438

1

Proton acceptor By similarity

Amino acid modifications

Modified residue

508

1

Phosphoserine By similarity

Modified residue

510

1

Phosphoserine By similarity

Modified residue

673

1

Phosphothreonine By similarity

Modified residue

682

1

Phosphothreonine By similarity

Modified residue

692

1

Phosphoserine By similarity

Modified residue

694

1

Phosphoserine By similarity

Modified residue

766

1

Phosphoserine By similarity

Modified residue

801

1

Phosphoserine By similarity

Modified residue

804

1

Phosphothreonine By similarity

Modified residue

807

1

Phosphoserine By similarity

Modified residue

846

1

Phosphothreonine By similarity

Modified residue

861

1

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q2LZB1 [UniParc].

Last modified October 14, 2008. Version 2.
Checksum: A479D84A1DBFE65D
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FASTA

1,059

116,372

        10         20         30         40         50         60 
MPVSMAVCET ANVVNAALRE SLGVGNGSRA ADEAKKTGGG GGDDSDSEMH NQIAVSAYAK 

        70         80         90        100        110        120 
RILMSKIEYE EVPNYHDSVL EQLKNKYIVI KQPSNNNNSS SCNGSNFGNS KVVGANGHDN 

       130        140        150        160        170        180 
GNNGHRKLTQ SESTQSGPSP NELPKPKRVL YPRENIRIGW KQSERKWQVG TGMINVGNTC 

       190        200        210        220        230        240 
YLNSTLQALF HIPALANWLV SEQTHLENCN ITESNGGCII CAMAKTLQST QSCQSAMRPF 

       250        260        270        280        290        300 
HIYSKLKQIC KHMVVGRQED AHEFLRFLVE AMEKAYLMRF RNFKELDQLV KETTPLNQIF 

       310        320        330        340        350        360 
GGYLRSEVRC LSCSHVSITF QHFQDLLLDI RKSDTLEEAF DGYFSRERLE DMGYKCEGCK 

       370        380        390        400        410        420 
KKVSATKQFS LERAPITLCI QLKRFSMIGN KLTKTISFKP RIDLSRFAAR SPAAAAQPLT 

       430        440        450        460        470        480 
YRLVSMVTHL GVSQHCGHYT AIGMTESGSY YNFDDSYVRP IAMQSVCNTN AYIMFYELDL 

       490        500        510        520        530        540 
SQTTPLKSNG LRLTNGHSQV AVPATVSSSS PTHTRFIGPQ LPPGGINGYS NGHATGSSNA 

       550        560        570        580        590        600 
QKTAIQFKQQ QQHPQQNGLQ VGTGKFQEPP HAKSPLAGAY NKGEAFPATT ANGNKSSSSS 

       610        620        630        640        650        660 
ASNSNHNKSV NQLQHQQHYL PISSEDEDSE DGATATATAT ATARPTAQLP SMPKMTEDSS 

       670        680        690        700        710        720 
DKPKTPLKSS VKTNLVKSLL KTPLKSLVPY ESASEEDEPL PNPRKRRSDS DSNDSGDSDP 

       730        740        750        760        770        780 
QPGHVNGHTK TNGGSLTNGN GLGKAKTILA TSSSSSLASA SASAASDDED ADEEEENSKL 

       790        800        810        820        830        840 
TNGWQPQKQS QSLTQSKAPP SPKTPPSPAV IKSKTGIWKV TRNDDNEDED DDDDEDEEEQ 

       850        860        870        880        890        900 
HQVVSTPSKN PRNPFAKSST TPGAKRQKLL NGIAVKSQQQ PRVGNGYQSE ASTNGNVVNE 

       910        920        930        940        950        960 
LLKQTHRGYG SASVLSWSGK PAELEKELVA EAREQRQHDH DDEEENEMDR GRQRKVKSAT 

       970        980        990       1000       1010       1020 
AKAYNSSTPG YNPFQEYESQ KRWHKSSNGG GSFPRYHNQN FRQNFQQRNK FKYNRFGGPG 

      1030       1040       1050 
GAKFQQQRAL QRHLASGGVF NRRQPTGQQQ QQQSQQSSS

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References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.

Gibbs R.A.
Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MV2-25 / Tucson 14011-0121.94.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH379069 Genomic DNA. Translation: EAL29597.2.
RefSeq	XP_001353862.2. XM_001353826.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	7237.FBpp0274209.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0275771; FBpp0274209; FBgn0078933.
GeneID	4813226.
KEGG	dpo:Dpse_GA18934.
Organism-specific databases
FlyBase	FBgn0078933. Dpse\GA18934.
Phylogenomic databases
eggNOG	COG5533.
InParanoid	Q2LZB1.
KO	K11855.
OrthoDB	EOG4WM38P.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP36_DROPS

Accession

Primary (citable) accession number: Q2LZB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	October 14, 2008
Last modified:	May 29, 2013
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents