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Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene

Usp36

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei180 – 1801NucleophilePROSITE-ProRule annotation
Active sitei438 – 4381Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 36
Protein scrawny
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene namesi
Name:Usp36
Synonyms:scny
ORF Names:GA18934
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001819 Componenti: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0078933. Dpse\GA18934.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10591059Ubiquitin carboxyl-terminal hydrolase 36PRO_0000378500Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei508 – 5081PhosphoserineBy similarity
Modified residuei510 – 5101PhosphoserineBy similarity
Modified residuei673 – 6731PhosphothreonineBy similarity
Modified residuei682 – 6821PhosphothreonineBy similarity
Modified residuei692 – 6921PhosphoserineBy similarity
Modified residuei694 – 6941PhosphoserineBy similarity
Modified residuei766 – 7661PhosphoserineBy similarity
Modified residuei801 – 8011PhosphoserineBy similarity
Modified residuei804 – 8041PhosphothreonineBy similarity
Modified residuei807 – 8071PhosphoserineBy similarity
Modified residuei846 – 8461PhosphothreonineBy similarity
Modified residuei861 – 8611PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with atms/PAF1, but not with CycT.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 479309USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
InParanoidiQ2LZB1.
KOiK11855.
OMAiNEMDRGR.
OrthoDBiEOG7XDBF6.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2LZB1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVSMAVCET ANVVNAALRE SLGVGNGSRA ADEAKKTGGG GGDDSDSEMH
60 70 80 90 100
NQIAVSAYAK RILMSKIEYE EVPNYHDSVL EQLKNKYIVI KQPSNNNNSS
110 120 130 140 150
SCNGSNFGNS KVVGANGHDN GNNGHRKLTQ SESTQSGPSP NELPKPKRVL
160 170 180 190 200
YPRENIRIGW KQSERKWQVG TGMINVGNTC YLNSTLQALF HIPALANWLV
210 220 230 240 250
SEQTHLENCN ITESNGGCII CAMAKTLQST QSCQSAMRPF HIYSKLKQIC
260 270 280 290 300
KHMVVGRQED AHEFLRFLVE AMEKAYLMRF RNFKELDQLV KETTPLNQIF
310 320 330 340 350
GGYLRSEVRC LSCSHVSITF QHFQDLLLDI RKSDTLEEAF DGYFSRERLE
360 370 380 390 400
DMGYKCEGCK KKVSATKQFS LERAPITLCI QLKRFSMIGN KLTKTISFKP
410 420 430 440 450
RIDLSRFAAR SPAAAAQPLT YRLVSMVTHL GVSQHCGHYT AIGMTESGSY
460 470 480 490 500
YNFDDSYVRP IAMQSVCNTN AYIMFYELDL SQTTPLKSNG LRLTNGHSQV
510 520 530 540 550
AVPATVSSSS PTHTRFIGPQ LPPGGINGYS NGHATGSSNA QKTAIQFKQQ
560 570 580 590 600
QQHPQQNGLQ VGTGKFQEPP HAKSPLAGAY NKGEAFPATT ANGNKSSSSS
610 620 630 640 650
ASNSNHNKSV NQLQHQQHYL PISSEDEDSE DGATATATAT ATARPTAQLP
660 670 680 690 700
SMPKMTEDSS DKPKTPLKSS VKTNLVKSLL KTPLKSLVPY ESASEEDEPL
710 720 730 740 750
PNPRKRRSDS DSNDSGDSDP QPGHVNGHTK TNGGSLTNGN GLGKAKTILA
760 770 780 790 800
TSSSSSLASA SASAASDDED ADEEEENSKL TNGWQPQKQS QSLTQSKAPP
810 820 830 840 850
SPKTPPSPAV IKSKTGIWKV TRNDDNEDED DDDDEDEEEQ HQVVSTPSKN
860 870 880 890 900
PRNPFAKSST TPGAKRQKLL NGIAVKSQQQ PRVGNGYQSE ASTNGNVVNE
910 920 930 940 950
LLKQTHRGYG SASVLSWSGK PAELEKELVA EAREQRQHDH DDEEENEMDR
960 970 980 990 1000
GRQRKVKSAT AKAYNSSTPG YNPFQEYESQ KRWHKSSNGG GSFPRYHNQN
1010 1020 1030 1040 1050
FRQNFQQRNK FKYNRFGGPG GAKFQQQRAL QRHLASGGVF NRRQPTGQQQ

QQQSQQSSS
Length:1,059
Mass (Da):116,372
Last modified:October 14, 2008 - v2
Checksum:iA479D84A1DBFE65D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH379069 Genomic DNA. Translation: EAL29597.2.
RefSeqiXP_001353862.2. XM_001353826.2.

Genome annotation databases

EnsemblMetazoaiFBtr0275771; FBpp0274209; FBgn0078933.
GeneIDi4813226.
KEGGidpo:Dpse_GA18934.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH379069 Genomic DNA. Translation: EAL29597.2.
RefSeqiXP_001353862.2. XM_001353826.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0275771; FBpp0274209; FBgn0078933.
GeneIDi4813226.
KEGGidpo:Dpse_GA18934.

Organism-specific databases

FlyBaseiFBgn0078933. Dpse\GA18934.

Phylogenomic databases

eggNOGiCOG5533.
InParanoidiQ2LZB1.
KOiK11855.
OMAiNEMDRGR.
OrthoDBiEOG7XDBF6.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
    Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
    , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
    Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MV2-25 / Tucson 14011-0121.94.

Entry informationi

Entry nameiUBP36_DROPS
AccessioniPrimary (citable) accession number: Q2LZB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: October 14, 2008
Last modified: July 22, 2015
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.