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Q2LYK1

- LIAS_DROPS

UniProt

Q2LYK1 - LIAS_DROPS

Protein

Lipoyl synthase, mitochondrial

Gene

Las

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (21 Feb 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi101 – 1011Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi112 – 1121Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi132 – 1321Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi136 – 1361Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi139 – 1391Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LasUniRule annotation
    ORF Names:GA18753
    OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
    Taxonomic identifieri46245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001819: Partially assembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0078753. Dpse\GA18753.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 374Lipoyl synthase, mitochondrialPRO_0000398221
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi7237.FBpp0275593.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2LYK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    InParanoidiQ2LYK1.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiQ2LYK1.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2LYK1-1 [UniParc]FASTAAdd to Basket

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    MLTAFRPNSP TAIVVRAAST NAKKLEEIRE RLAKGPNFQD FVQNPDNLKS    50
    VGENYDGKLR REKGEEQRLR LPPWLKTTIP MGKNYTKIKE QLRELKLSTV 100
    CEEARCPNIG ECWGGGENGT QTATIMLMGD TCTRGCRFCS VKTARIPPPL 150
    DENEPVNTAK AIASWGLDYI VLTSVDRDDL PDGGSKHIAQ TVREIKARNS 200
    NIFVECLVPD FRGDLDCVET IANCGLDVYA HNIETVEKLT PYVRDRRAHY 250
    RQTLRVLTEA KRFNPNLITK SSIMLGLGET DEEIERTMTD LREAGVECLT 300
    LGQYMQPTNK HLKVIEYVTP EKFKHWEERG NDLGFLYTAS GPLVRSSYKA 350
    GEFFISSILA NRKANQSKDS APKN 374
    Length:374
    Mass (Da):42,118
    Last modified:February 21, 2006 - v1
    Checksum:iE27E02B8B2B5A9F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH379069 Genomic DNA. Translation: EAL29861.1.
    RefSeqiXP_001354122.1. XM_001354086.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0277155; FBpp0275593; FBgn0078753.
    GeneIDi4813963.
    KEGGidpo:Dpse_GA18753.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH379069 Genomic DNA. Translation: EAL29861.1 .
    RefSeqi XP_001354122.1. XM_001354086.1.

    3D structure databases

    ProteinModelPortali Q2LYK1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7237.FBpp0275593.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0277155 ; FBpp0275593 ; FBgn0078753 .
    GeneIDi 4813963.
    KEGGi dpo:Dpse_GA18753.

    Organism-specific databases

    FlyBasei FBgn0078753. Dpse\GA18753.

    Phylogenomic databases

    eggNOGi COG0320.
    InParanoidi Q2LYK1.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi Q2LYK1.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
      Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
      , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
      Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MV2-25 / Tucson 14011-0121.94.

    Entry informationi

    Entry nameiLIAS_DROPS
    AccessioniPrimary (citable) accession number: Q2LYK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: February 21, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3