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Q2LY09 (BIOB_SYNAS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:SYNAS_30920
ORF Names:SYN_00212
OrganismSyntrophus aciditrophicus (strain SB) [Complete proteome] [HAMAP]
Taxonomic identifier56780 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophaceaeSyntrophus

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325Biotin synthase HAMAP-Rule MF_01694
PRO_0000381671

Sites

Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1141Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1461Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2061Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2LY09 [UniParc].

Last modified February 21, 2006. Version 1.
Checksum: A6F717053599885E

FASTA32535,487
        10         20         30         40         50         60 
MILDSTVNML AQKILDEGPQ ALSFQEACSL TELPARSIPD LFLAACKIRQ HYQKDDVVLC 

        70         80         90        100        110        120 
SILNAKSGAC AENCAFCSQS GHHRTDVVKR PLLSAEAMIE EARRLDSAGA TRFSMVTSGS 

       130        140        150        160        170        180 
RLNAAEIETV GQAASAITKS TRLTVCASLG QLTASSAERL KAGGVSVYHH NLETARSFFP 

       190        200        210        220        230        240 
QICTTHDYEE DIETLLIARK AGLRLCSGGI FGLGESWEQR VEMAFTLREL DVDSIPINFL 

       250        260        270        280        290        300 
NPLPGTRMAD RSLLPPMEAL QVIALYRFIH PAKPLLICGG REITLRDFQS WIFLAGASGM 

       310        320 
IVGNYLTTQG RDIGMDRDMI QSGLF 

« Hide

References

[1]"The genome of the syntrophic bacterium Syntrophus aciditrophicus: Life dependent on negative change in electrical potential."
Gunsalus R., Rohlin L., Kim U., Krupp R., Bhattacharyya A., Campbell J., Mclerney M., Moutakki H., Rio-Hernandez L.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SB.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000252 Genomic DNA. Translation: ABC78971.1.
RefSeqYP_463139.1. NC_007759.1.

3D structure databases

ProteinModelPortalQ2LY09.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING56780.SYN_00212.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABC78971; ABC78971; SYN_00212.
GeneID3883247.
KEGGsat:SYN_00212.
PATRIC23867108. VBISynAci70500_3332.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycSACI56780:GHXT-3141-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SYNAS
AccessionPrimary (citable) accession number: Q2LY09
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 21, 2006
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways