Q2LUT2 (SYE2_SYNAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	SYNAS_19610
ORF Names:	SYN_01399

Organism

Syntrophus aciditrophicus (strain SB) [Complete proteome] [HAMAP]

Taxonomic identifier

56780 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Syntrophobacterales › Syntrophaceae › Syntrophus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000237414
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LUT2 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: 713BE8894F195C91	FASTA	486	55,617
	10 20 30 40 50 60 MNSTKPRVRF APSPTGELHI GNARTAFFNW LYARHYGGKL ILRIEDTDRQ RSTRAFEARL 70 80 90 100 110 120 IDDLKWLSLD WDEGPDGKGE VGPYRQSERL DLYESFLKNL QKDGRVYPCY CTEDELELER 130 140 150 160 170 180 TSLLSRKMAP RYMGKCRNLT EADRRRLEAQ GRRPTWRFRV SQGPVLFQDL IRGTMKFQGE 190 200 210 220 230 240 AVGDFIIVRS NGTPAYNFAV VIDDHFMEIS TVIRGEDHLS NTAIQLMLYE ALGFEPPEFA 250 260 270 280 290 300 HHSLILGKDR TKLSKRHGSV SVREFREKGI LPEALLNYLA LLGSSIGEGR EVCSLEEIIT 310 320 330 340 350 360 AFSLDRAGKS GAVFDEDKLL WMNSLYIHEE PAIKLIERLR PFIEKAGYDV NKWETPWLDR 370 380 390 400 410 420 MVEAVKPNLT TLADIGSYVK MIVEEPVRID EDAAAVLRET ETQMVLRTLL QLIEEGKFSH 430 440 450 460 470 480 EDFYSQVMTA LRKVTGARGK RLFMPVRAAL TGTTRGPELD KIFVLLGEQS VKERLKKALN MQGNFS

References

[1]

"The genome of the syntrophic bacterium Syntrophus aciditrophicus: Life dependent on negative change in electrical potential." Gunsalus R., Rohlin L., Kim U., Krupp R., Bhattacharyya A., Campbell J., Mclerney M., Moutakki H., Rio-Hernandez L. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000252 Genomic DNA. Translation: ABC77840.1.
RefSeq	YP_462008.1. NC_007759.1.
3D structure databases
ProteinModelPortal	Q2LUT2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2LUT2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3882794.
GenomeReviews	Gene locus SYNAS_19610 in contig CP000252_GR.
KEGG	sat:SYN_01399.
NMPDR	fig|56780.10.peg.1951.
PATRIC	23864670. VBISynAci70500_2120.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	QDLIRGT.
PhylomeDB	Q2LUT2.
Enzyme and pathway databases
BioCyc	SACI56780:SYN_01399-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_SYNAS

Accession

Primary (citable) accession number: Q2LUT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 21, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program