Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Argininosuccinate synthase

Gene

argG

Organism
Syntrophus aciditrophicus (strain SB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei89 – 891CitrullineUniRule annotation
Binding sitei94 – 941CitrullineUniRule annotation
Binding sitei119 – 1191ATP; via amide nitrogenUniRule annotation
Binding sitei121 – 1211AspartateUniRule annotation
Binding sitei125 – 1251AspartateUniRule annotation
Binding sitei125 – 1251CitrullineUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei129 – 1291CitrullineUniRule annotation
Binding sitei180 – 1801CitrullineUniRule annotation
Binding sitei189 – 1891CitrullineUniRule annotation
Binding sitei265 – 2651CitrullineUniRule annotation
Binding sitei277 – 2771CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 199ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSACI56780:GHXT-1462-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:SYNAS_14290
ORF Names:SYN_02157
OrganismiSyntrophus aciditrophicus (strain SB)
Taxonomic identifieri56780 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophaceaeSyntrophus
ProteomesiUP000001933 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Argininosuccinate synthasePRO_0000263987Add
BLAST

Proteomic databases

PRIDEiQ2LT97.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi56780.SYN_02157.

Structurei

3D structure databases

ProteinModelPortaliQ2LT97.
SMRiQ2LT97. Positions 7-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2LT97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTGVKKVVL AYSGGLDTSV IVQWLIETYG CEVIAFAADV GQKEELEGLR
60 70 80 90 100
EKAIKTGASK IYIEDLREEF ARDFVFPALR ANAIYEGTYL LGTSLARPLI
110 120 130 140 150
AKRQVEIARA EGADAVCHGA TGKGNDQVRF ELTYMALEPN IRIISAWKDP
160 170 180 190 200
NWHFKSREDM FDYAEKHNIP LPLTREKPYS SDRNLLHISH EGAILEDPWA
210 220 230 240 250
EPPEDIFTIS LSPEAAPDKP TYVEIDFEQG NPVALDGARL SPAALMEKMN
260 270 280 290 300
DIAGANGIGR VDMVENRFVG MKSRGVYETP GGTVLWAAHR ALESLTMDRE
310 320 330 340 350
VMLMRDSLIP KYAQLAYNGF WYAPEMEALQ ALIDETQKKA TGTVRMKLYK
360 370 380 390 400
GNSIVVGRKS PYSLYSEDFA TFEKDQVYNQ MDATGFIRLN ALRLRIAAMK

K
Length:401
Mass (Da):44,906
Last modified:February 21, 2006 - v1
Checksum:iCC615D17B72F3E99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000252 Genomic DNA. Translation: ABC77308.1.
RefSeqiWP_011417330.1. NC_007759.1.

Genome annotation databases

EnsemblBacteriaiABC77308; ABC77308; SYN_02157.
KEGGisat:SYN_02157.
PATRICi23863502. VBISynAci70500_1549.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000252 Genomic DNA. Translation: ABC77308.1.
RefSeqiWP_011417330.1. NC_007759.1.

3D structure databases

ProteinModelPortaliQ2LT97.
SMRiQ2LT97. Positions 7-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi56780.SYN_02157.

Proteomic databases

PRIDEiQ2LT97.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC77308; ABC77308; SYN_02157.
KEGGisat:SYN_02157.
PATRICi23863502. VBISynAci70500_1549.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciSACI56780:GHXT-1462-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the syntrophic bacterium Syntrophus aciditrophicus: Life dependent on negative change in electrical potential."
    Gunsalus R., Rohlin L., Kim U., Krupp R., Bhattacharyya A., Campbell J., Mclerney M., Moutakki H., Rio-Hernandez L.
    Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SB.

Entry informationi

Entry nameiASSY_SYNAS
AccessioniPrimary (citable) accession number: Q2LT97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: February 21, 2006
Last modified: July 22, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.