Q2LR78 (PROB_SYNAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	SYNAS_07070
ORF Names:	SYN_01350

Organism

Syntrophus aciditrophicus (strain SB) [Complete proteome] [HAMAP]

Taxonomic identifier

56780 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Syntrophobacterales › Syntrophaceae › Syntrophus › Syntrophus aciditrophicus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000253013
Regions
	Domain	283 – 361	79	PUA
	Nucleotide binding	218 – 224	7	ATP By similarity
Sites
	Binding site	18	1	ATP By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	156	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LR78 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: EB7693F3FB5B0167	FASTA	385	42,139
	10 20 30 40 50 60 MNENREQVLK NVKRVLIKIG SAVLTGDNGL DLERIQHLVD QMAALTHRGY QVVMVTSGAI 70 80 90 100 110 120 ASGKHRLGIT TALKSIPQKQ AAAAIGQGRL MRIYSNSFGK HGLYVGQILL TMSDLTDRRR 130 140 150 160 170 180 FLNIRNTLST LMEWGIIAII NENDTVAIDE IKFGDNDNLA AMIANIIEAH LVINLTSTPG 190 200 210 220 230 240 LYDRNPASSR NARLIPLVRE ITEDIEAAAS EEGTSVGTGG MKSKVMAAKK VTAFGIPYII 250 260 270 280 290 300 APGKQKDVLL DIFDGNELGT LFLPMREHLS SRKYWIAFTL RSRGVLSIDA GARTAILEEG 310 320 330 340 350 360 KSLLPSGIVG VEGDFIVGDP VTCVDRDGVP LAKGLVNYSA PDIRKIMGLK TSRIEQVLGH 370 380 KDYDEIIHRD NLAVIRRSRR HREAG

References

[1]

"The genome of the syntrophic bacterium Syntrophus aciditrophicus: Life dependent on negative change in electrical potential." Gunsalus R., Rohlin L., Kim U., Krupp R., Bhattacharyya A., Campbell J., Mclerney M., Moutakki H., Rio-Hernandez L. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000252 Genomic DNA. Translation: ABC76586.1.
RefSeq	YP_460754.1. NC_007759.1.
3D structure databases
ProteinModelPortal	Q2LR78.
ModBase	Search...
Protein-protein interaction databases
STRING	56780.SYN_01350.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3884768.
KEGG	sat:SYN_01350.
PATRIC	23861905. VBISynAci70500_0769.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	VYTGNPK.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	SACI56780:GHXT-722-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	2.30.130.10. 1 hit. 3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_SYNAS

Accession

Primary (citable) accession number: Q2LR78

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	February 21, 2006
Last modified:	May 29, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program