Q2LR65 (CHEB1_SYNAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	SYNAS_06930
ORF Names:	SYN_00430

Organism

Syntrophus aciditrophicus (strain SB) [Complete proteome] [HAMAP]

Taxonomic identifier

56780 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Syntrophobacterales › Syntrophaceae › Syntrophus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.
Sequence caution	The sequence ABC76572.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264328
Regions
	Domain	3 – 121	119	Response regulatory
	Domain	148 – 348	201	CheB-type methylesterase
Sites
	Active site	170	1	By similarity
	Active site	197	1	By similarity
	Active site	290	1	By similarity
Amino acid modifications
	Modified residue	54	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LR65 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 01CBB6A4700F2CAB	FASTA	350	37,995
	10 20 30 40 50 60 MIKVLIVEDS PVVRELLSHI LHSDPQIRVV GVAESGEEAL KEVVRLRPDL ITMDVNLPRM 70 80 90 100 110 120 DGFETTRRIM EEIPTPIIIV SAAWVSTEVE KTFRALEAGA LAVLEKPSIA ARDYDIRARD 130 140 150 160 170 180 LIRAVKAMSE VKVIRRWSRS PRIEPAPPAS GEPDIGKITK RFEVVAMGAS TGGPTVLKQI 190 200 210 220 230 240 LSDLPDNFTI PILIVQHMTQ GFIPGFVDWL SRAANYPVSV AVHGEEIRPG HAYVAPDGLH 250 260 270 280 290 300 MGVNALGKII LTKGEPENGL CPSVSYLFRS VYRAYGENAV GVLLTGMGKD GAYELKVMKD 310 320 330 340 350 CGMITIAQNK ETSVVYGMPG EAIGMNAASY VLPPEKIARV LVKLAGSVAP

References

[1]

"The genome of the syntrophic bacterium Syntrophus aciditrophicus: Life dependent on negative change in electrical potential." Gunsalus R., Rohlin L., Kim U., Krupp R., Bhattacharyya A., Campbell J., Mclerney M., Moutakki H., Rio-Hernandez L. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000252 Genomic DNA. Translation: ABC76572.1. Different initiation.
RefSeq	YP_460740.1. NC_007759.1.
3D structure databases
ProteinModelPortal	Q2LR65.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2LR65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3885165.
GenomeReviews	Gene locus SYNAS_06930 in contig CP000252_GR.
KEGG	sat:SYN_00430.
NMPDR	fig|56780.10.peg.686.
PATRIC	23861873. VBISynAci70500_0754.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
PhylomeDB	Q2LR65.
Enzyme and pathway databases
BioCyc	SACI56780:SYN_00430-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_SYNAS

Accession

Primary (citable) accession number: Q2LR65

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program