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Protein

Enolase

Gene

eno

Organism
Syntrophus aciditrophicus (strain SB)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. no protein annotated in this organism
  4. Enolase (eno)
  5. Pyruvate kinase (SYN_02952)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155SubstrateUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Active sitei205Proton donorUniRule annotation1
Metal bindingi242MagnesiumUniRule annotation1
Metal bindingi286MagnesiumUniRule annotation1
Binding sitei286SubstrateUniRule annotation1
Metal bindingi313MagnesiumUniRule annotation1
Binding sitei313SubstrateUniRule annotation1
Active sitei338Proton acceptorUniRule annotation1
Binding sitei338Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei389SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:SYNAS_06680
ORF Names:SYN_01733
OrganismiSyntrophus aciditrophicus (strain SB)
Taxonomic identifieri56780 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaSyntrophobacteralesSyntrophaceaeSyntrophus
Proteomesi
  • UP000001933 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002671281 – 428EnolaseAdd BLAST428

Proteomic databases

PRIDEiQ2LR33.

Interactioni

Protein-protein interaction databases

STRINGi56780.SYN_01733.

Structurei

3D structure databases

ProteinModelPortaliQ2LR33.
SMRiQ2LR33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 368Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70. Bacteria.
COG0148. LUCA.
HOGENOMiHOG000072174.
KOiK01689.
OMAiEFMIIPV.
OrthoDBiPOG091H02DK.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2LR33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEIIEVHAR EILDSRGNPT VEAEVTLLSG ISGRASVPSG ASTGEHEMLE
60 70 80 90 100
LRDGDPKRYL GKGVTQAVNN VIEKIAPEIV GMDCLNQRDI DYTMIALDGT
110 120 130 140 150
ENKGALGANA ILSVSIACAK AAAAVVELPL YRYLGGVQAK DIPVPMMNII
160 170 180 190 200
NGGQHADNNV DIQEFMIMPV GAPTFREGLR MSAEVFHNLK AVLKGKGYNT
210 220 230 240 250
AVGDEGGFAP NLASNEEALA VIMAAITRAG YEPGKDIAIA LDAAASSFYE
260 270 280 290 300
KGKYILAAEK KPEKTADEMI RFYEDLANRY PIISLEDGLA EDDWEGWKAL
310 320 330 340 350
TQAMGSRIQI VGDDLFVTNK KRLEQGISKG IANSILIKLN QIGSLTETLE
360 370 380 390 400
TIQTAKEAGY TNVVSHRSGE TEDSFMADVA VAANCGQIKS GSLSRSERLA
410 420
KYNQLLRIEE ELGDRAVYRG RSALYSVR
Length:428
Mass (Da):46,190
Last modified:February 21, 2006 - v1
Checksum:iDEF2AA63E8A045A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000252 Genomic DNA. Translation: ABC76547.1.
RefSeqiWP_011416581.1. NC_007759.1.

Genome annotation databases

EnsemblBacteriaiABC76547; ABC76547; SYN_01733.
KEGGisat:SYN_01733.
PATRICi23861821. VBISynAci70500_0728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000252 Genomic DNA. Translation: ABC76547.1.
RefSeqiWP_011416581.1. NC_007759.1.

3D structure databases

ProteinModelPortaliQ2LR33.
SMRiQ2LR33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi56780.SYN_01733.

Proteomic databases

PRIDEiQ2LR33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC76547; ABC76547; SYN_01733.
KEGGisat:SYN_01733.
PATRICi23861821. VBISynAci70500_0728.

Phylogenomic databases

eggNOGiENOG4105C70. Bacteria.
COG0148. LUCA.
HOGENOMiHOG000072174.
KOiK01689.
OMAiEFMIIPV.
OrthoDBiPOG091H02DK.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_SYNAS
AccessioniPrimary (citable) accession number: Q2LR33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: February 21, 2006
Last modified: April 12, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.