Reviewed, UniProtKB/Swiss-Prot Q2LQN4 (SYE1_SYNAS)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	SYNAS_01920
ORF Names:	SYN_02557

Organism

Syntrophus aciditrophicus (strain SB) [Complete proteome] [HAMAP]

Taxonomic identifier

56780 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Syntrophobacterales › Syntrophaceae › Syntrophus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000237413
Regions
	Motif	13 – 23	11	"HIGH" region HAMAP MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022
Sites
	Metal binding	102	1	Zinc By similarity
	Metal binding	104	1	Zinc By similarity
	Metal binding	129	1	Zinc By similarity
	Metal binding	131	1	Zinc By similarity
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LQN4-1 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: 4A1ED73395B51DE3	FASTA	472	53,248
	10 20 30 40 50 60 MQKLKEIRTR FAPSPTGYLH IGGARTALFS WLYARHHQGK FVLRIEDTDQ LRSTEESTRA 70 80 90 100 110 120 ILDAMTWLGL NWDEGPVFQA ERVDIHRAMI RKLVDEDKAY YCTCTPDELE EKRKRALAEG 130 140 150 160 170 180 RKPKYDGTCR EKKLPPSPGT VVRFRCPQTG ITVVDDLIKG KISFNNEELD DLIIQRSDGY 190 200 210 220 230 240 PTYNFAVVVD DAQMGISHVI RGDDHVNNTP RQILLYQALG YDIPHFGHVP MILGADKARL 250 260 270 280 290 300 SKRHGATSVM AYKDMGYLPE ALVNYLVRLG WSHGDQEIFS LDELIALFGL ESIGKSAAVF 310 320 330 340 350 360 NPEKLLWLNQ HYIKTYPEDR LLEVLQPFWK QLGIEAPDPD YGRSIVRDLR ARAKTLVDMA 370 380 390 400 410 420 ESSTFYFNDE PAIDADAAKK FLTPEIAGHL EAIAEALATL GDYSKEGIEI FLRSLVEARA 430 440 450 460 470 IKLKTIAQPL RIALTGKTVS PGLDDIMLTL GKERVIARIQ RTVAYIRSGM AS

References

[1]

"The genome of the syntrophic bacterium Syntrophus aciditrophicus: Life dependent on negative change in electrical potential." Gunsalus R., Rohlin L., Kim U., Krupp R., Bhattacharyya A., Campbell J., Mclerney M., Moutakki H., Rio-Hernandez L. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000252 Genomic DNA. Translation: ABC76071.1.
RefSeq	YP_460239.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3884491.
GenomeReviews	Gene locus SYNAS_01920 in contig CP000252_GR.
KEGG	sat:SYN_02557.
NMPDR	fig|56780.10.peg.190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2LQN4.
OMA	Q2LQN4. MAHIPLI.
Enzyme and pathway databases
BioCyc	SACI56780:SYN_02557-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_SYNAS

Accession

Primary (citable) accession number: Q2LQN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 21, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)