Reviewed, UniProtKB/Swiss-Prot Q2LPW5 (SYD1_SYNAS)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase 1
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase 1
      Short name=AspRS 1

Gene names

Name:	aspS1
Ordered Locus Names:	SYNAS_04480
ORF Names:	SYN_02602

Organism

Syntrophus aciditrophicus (strain SB) [Complete proteome] [HAMAP]

Taxonomic identifier

56780 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Syntrophobacterales › Syntrophaceae › Syntrophus

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity. HAMAP MF_00044
Subcellular location	Cytoplasm HAMAP MF_00044.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Aspartyl-tRNA synthetase 1 HAMAP MF_00044		PRO_0000235571

Sequences

	Sequence		Length	Mass (Da)
	Q2LPW5-1 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: A62EAFBD7D3303FF	FASTA	723	80,508
	10 20 30 40 50 60 MPNRGAHFSI SRRKDFLMDF SERVFCGHLT PDHTGRRVLL AGWVDAFRDH GGLLFIHLRD 70 80 90 100 110 120 RNGIIQIVFS PEAASADVYR QAASLRAEYC VAVQGEVRKR LPGTENPHIE TGDIEVFVSE 130 140 150 160 170 180 LTVLSESEAL PFAISDKAMV AGASSAGADH VNEDLRMQYR YLDIRRPAMQ KNLILRHRIS 190 200 210 220 230 240 QCVREFLDSR GFVEVETPVL TMSTPEGARD YLVPSRIHPR SFYALPQSPQ LFKQLLMIGG 250 260 270 280 290 300 MERYFQLARC FRDEDLRPNR QPEFTQLDIE ASFIDEEFLY ELIEELTVRM FAIGGIALSR 310 320 330 340 350 360 PFPRMTYAEA MDTTGSDRPD LRFGLRMADV TGVFSRTSYS IFKQILQRGG SIKGINIKGQ 370 380 390 400 410 420 SEKLSKNVLQ NEYAKEIAPS FGAKGMTWMR AEEGKLESNI VQFFSADELE ALKRVFQVED 430 440 450 460 470 480 GDVLIMVADP SCAIVNSALG QLRLHLGNRL GLIPEGVFYP VWITEFPLFE PTDEGGVTSS 490 500 510 520 530 540 HHPFTAPDRT DFDPGNIEEL LSLRSRAYDL VVNGEELGGG SIRINDREVQ RRIFAALGLT 550 560 570 580 590 600 EEDVKNKFGF FLRAFDFAAP PHGGLALGMD RVVSMILQTP SIREVIAFPK NRSAACPLTG 610 620 630 640 650 660 APSEVKREQL AELGLLNLGD KDVLPGDAEK EDRIDHLSWV SRIGIAEGER PVMESILAQA 670 680 690 700 710 720 EELAAQVGDL AGNEEPVRSV APVANRVREG LEAVRLSFSG TGRLLKNAPA VKGDYFKVAG ILD

References

[1]

"The genome of the syntrophic bacterium Syntrophus aciditrophicus: Life dependent on negative change in electrical potential." Gunsalus R., Rohlin L., Kim U., Krupp R., Bhattacharyya A., Campbell J., Mclerney M., Moutakki H., Rio-Hernandez L. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000252 Genomic DNA. Translation: ABC76327.1.
RefSeq	YP_460495.1.
3D structure databases
SMR	Q2LPW5. Positions 24-616, 631-723.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2LPW5.
Genome annotation databases
GeneID	3885262.
GenomeReviews	Gene locus SYNAS_04480 in contig CP000252_GR.
KEGG	sat:SYN_02602.
NMPDR	fig|56780.10.peg.448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	NEYAKEI.
Enzyme and pathway databases
BioCyc	SACI56780:SYN_02602-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. Divergent sequence. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD1_SYNAS

Accession

Primary (citable) accession number: Q2LPW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	February 21, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)