Reviewed, UniProtKB/Swiss-Prot Q2LD53 (T23O_MAYDE)

Names and origin

Protein names	Recommended name:     Tryptophan 2,3-dioxygenase       Short name=TDO     EC=1.13.11.11 Alternative name(s):     Tryptophan pyrrolase       Short name=Tryptophanase     Tryptophan oxygenase       Short name=TRPO       Short name=TO     Tryptamin 2,3-dioxygenase
Organism	Mayetiola destructor (Hessian fly)
Taxonomic identifier	39758 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Sciaroidea › Cecidomyiidae › Mayetiola

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Tryptophan 2,3-dioxygenase		PRO_0000360885
Regions
	Region	30 – 34	5	Substrate binding By similarity
	Region	60 – 64	5	Substrate binding By similarity
Sites
	Metal binding	316	1	Iron (heme axial ligand) By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	138	1	Heme By similarity
	Binding site	331	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LD53-1 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: 518DE9C496DFE8B0	FASTA	389	45,610
	10 20 30 40 50 60 MACPYRSSAY TNGDHAQKGE VLGSEMGMLY GEYLMLNQII NSQRMQSVVS NRPVHDEHLF 70 80 90 100 110 120 IITHQAYELW FKQIIYELDS IRDLFNTITV DESRTLDILK RLNRIVMILK LLVDHIPILE 130 140 150 160 170 180 TMTPLDFMDF RGYLSPASGF QSLQFRLLEN KLGVKQEHRV KYNQKYQDVF GDDAKSLDML 190 200 210 220 230 240 TASEDEPSLC DLVQKWLERT PGLEESGFNF YQKFRSNVYE LLDREEQKAK MQPLEHIRNY 250 260 270 280 290 300 QLMDIKKRRD VYHTIFNEDA HEALVSRGER KFSYKAMQGA ILITLYRDEP RFSQPHQLLN 310 320 330 340 350 360 CLMDIDSFMT KWRYNHVMMV QRMIGSQQLG TGGSSGYQYL RSTLSDRYKV SLDLFNLSTF 370 380 LIPRSNIPPL TEEMSNQLNT NTRTIKPTN

References

[1]	"Cloning and characterization of white and vermilion eye-color genes from Hessian fly, Mayetiola destructor." Yoshiyama M., Morton P.K., Shukle R.H. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ335251 Genomic DNA. Translation: ABC69733.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_MAYDE

Accession

Primary (citable) accession number: Q2LD53

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	February 21, 2006
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)