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Protein

Zinc metalloproteinase-disintegrin-like TSV-DM

Gene
N/A
Organism
Trimeresurus stejnegeri (Chinese green tree viper) (Viridovipera stejnegeri)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom zinc metalloprotease that hydrolyzes the alpha-chain (FGA) and more slowly the beta-chain (FGB) of fibrinogen. Inhibits cell proliferation and induces cell morphologic changes transiently on human umbilical vein endothelial cells.1 Publication

Miscellaneous

Does not affect gamma-chain of fibrinogen (FGG). Does not show apoptosis effects (PubMed:16487560).1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by EDTA and DTT, and partially inhibited by EGTA, but not inhibited by PMSF and NEM.1 Publication

Kineticsi

  1. KM=25.0 µM for NFF-2 (fluorogenic substrates with cleavage at Ala-Nva)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi336Zinc; catalyticBy similarity1
    Active sitei337PROSITE-ProRule annotation1
    Metal bindingi340Zinc; catalyticBy similarity1
    Metal bindingi346Zinc; catalyticBy similarity1
    Metal bindingi406Calcium 1; via carbonyl oxygenBy similarity1
    Metal bindingi409Calcium 1By similarity1
    Metal bindingi411Calcium 1; via carbonyl oxygenBy similarity1
    Metal bindingi413Calcium 1By similarity1
    Metal bindingi416Calcium 1By similarity1
    Metal bindingi419Calcium 1By similarity1
    Metal bindingi470Calcium 2By similarity1
    Metal bindingi471Calcium 2; via carbonyl oxygenBy similarity1
    Metal bindingi473Calcium 2By similarity1
    Metal bindingi484Calcium 2By similarity1
    Metal bindingi485Calcium 2; via carbonyl oxygenBy similarity1

    GO - Molecular functioni

    Keywordsi

    Molecular functionCell adhesion impairing toxin, Fibrinogenolytic toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Protease, Toxin
    LigandCalcium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc metalloproteinase-disintegrin-like TSV-DM (EC:3.4.24.-)
    Alternative name(s):
    Snake venom metalloproteinase
    Short name:
    SVMP
    OrganismiTrimeresurus stejnegeri (Chinese green tree viper) (Viridovipera stejnegeri)
    Taxonomic identifieri39682 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeTrimeresurus

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 20Sequence analysisAdd BLAST20
    PropeptideiPRO_000034031021 – 191By similarityAdd BLAST171
    ChainiPRO_0000340311192 – 621Zinc metalloproteinase-disintegrin-like TSV-DMAdd BLAST430

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei192Pyrrolidone carboxylic acid1 Publication1
    Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi311 ↔ 391By similarity
    Disulfide bondi351 ↔ 375By similarity
    Disulfide bondi353 ↔ 358By similarity
    Disulfide bondi366Interchain (with C-366)PROSITE-ProRule annotation
    Disulfide bondi407 ↔ 436By similarity
    Disulfide bondi418 ↔ 431By similarity
    Disulfide bondi420 ↔ 426By similarity
    Disulfide bondi430 ↔ 453By similarity
    Disulfide bondi444 ↔ 450By similarity
    Disulfide bondi449 ↔ 475By similarity
    Disulfide bondi462 ↔ 482By similarity
    Disulfide bondi469 ↔ 500By similarity
    Disulfide bondi493 ↔ 505By similarity
    Glycosylationi502N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi512 ↔ 562By similarity
    Disulfide bondi527 ↔ 573By similarity
    Disulfide bondi540 ↔ 550By similarity
    Disulfide bondi557 ↔ 599By similarity
    Disulfide bondi593 ↔ 605By similarity

    Post-translational modificationi

    The N-terminus is blocked.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid, Zymogen

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ2LD49.
    SMRiQ2LD49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini200 – 396Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
    Domaini404 – 489DisintegrinPROSITE-ProRule annotationAdd BLAST86

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi468 – 470D/ECD-tripeptide3

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi490 – 621Cys-richAdd BLAST132

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG006978.

    Family and domain databases

    CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
    Gene3Di3.40.390.10. 1 hit.
    4.10.70.10. 1 hit.
    InterProiView protein in InterPro
    IPR006586. ADAM_Cys-rich.
    IPR018358. Disintegrin_CS.
    IPR001762. Disintegrin_dom.
    IPR036436. Disintegrin_dom_sf.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR002870. Peptidase_M12B_N.
    IPR034027. Reprolysin_adamalysin.
    PfamiView protein in Pfam
    PF08516. ADAM_CR. 1 hit.
    PF00200. Disintegrin. 1 hit.
    PF01562. Pep_M12B_propep. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PRINTSiPR00289. DISINTEGRIN.
    SMARTiView protein in SMART
    SM00608. ACR. 1 hit.
    SM00050. DISIN. 1 hit.
    SUPFAMiSSF57552. SSF57552. 1 hit.
    PROSITEiView protein in PROSITE
    PS50215. ADAM_MEPRO. 1 hit.
    PS00427. DISINTEGRIN_1. 1 hit.
    PS50214. DISINTEGRIN_2. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2LD49-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIQVLLVTIC LAVFPYQGSS IILESGNVND YEVVYPRKVT SLPKGAVQQK
    60 70 80 90 100
    YEDAMQYELK VNGEPVVLHL EKNKGLFSKD YSETHYSPDG RKITTKPPVK
    110 120 130 140 150
    DHCYYHGHIQ NDADSTASIS ACNGLKGHFK LQGEMYLIEP LKLPDSEAHA
    160 170 180 190 200
    VFKYENVEKE DEAPKMCGVT ETNWESDEPI KEASQSNLTP EQQSYLNAPK
    210 220 230 240 250
    YVKFFLVADH IMYLKYGRNL TTLRTRIFDT VNVVYLILLR INIHVLLVGM
    260 270 280 290 300
    EIWSHKDKII VQSVPAVTLK LFATWREADL LKHKSHGCAH LLTGINFNGP
    310 320 330 340 350
    TAGLAYLGAI CNPMYSAGIV QDHNKIHHLV AIAMAHELGH NLGINHDKDT
    360 370 380 390 400
    CTCRAKACVM AGTISCDASY LFSDCSRQEH REFLIKNMPQ CILKKPLKTD
    410 420 430 440 450
    VVSPPVCGNY FVEVGEDCDC GSPATCRDSC CNPTNCKLRQ GAQCAEGLCC
    460 470 480 490 500
    DQCRFKGAGT ECRPASSECD MADLCTGRSA ECTDRFQRNG QPCQNNNGYC
    510 520 530 540 550
    YNGTCPSMTD QCIALFGPNA AVSQDACFQF NREGNHYGYC RKEQNTKIAC
    560 570 580 590 600
    EPENVKCGRL YCIDSSPANK NPCNIVYLPN DEEKGMVLAG TKCADGRACN
    610 620
    SNGQCVGVNG AYKSTTGFSQ I
    Length:621
    Mass (Da):68,718
    Last modified:February 21, 2006 - v1
    Checksum:iF8C31FECC7B33F0E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ335449 mRNA. Translation: ABC73079.1.

    Similar proteinsi

    Entry informationi

    Entry nameiVM3TM_TRIST
    AccessioniPrimary (citable) accession number: Q2LD49
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: February 21, 2006
    Last modified: October 25, 2017
    This is version 66 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families