Reviewed, UniProtKB/Swiss-Prot Q2LCQ6 (COX1_DICCI)

Names and origin

Protein names

Recommended name:
    Cytochrome c oxidase subunit 1+2
    EC=1.9.3.1
Alternative name(s):
    Cytochrome c oxidase polypeptide I+II

Gene names

Name:

cox1/2

Encoded on

Mitochondrion

Organism

Dictyostelium citrinum (Slime mold)

Taxonomic identifier

361072 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B By similarity.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Pathway	Energy metabolism; oxidative phosphorylation.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	In the N-terminal section; belongs to the heme-copper respiratory oxidase family. In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane
Ligand	Copper Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from electronic annotation. Source: InterPro respiratory electron transport chain Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 773	773	Cytochrome c oxidase subunit 1+2		PRO_0000312386
Regions
	Transmembrane	41 – 61	21	Potential
	Transmembrane	87 – 111	25	Potential
	Transmembrane	130 – 150	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	211 – 231	21	Potential
	Transmembrane	262 – 278	17	Potential
	Transmembrane	290 – 310	21	Potential
	Transmembrane	335 – 355	21	Potential
	Transmembrane	362 – 382	21	Potential
	Transmembrane	396 – 416	21	Potential
	Transmembrane	444 – 464	21	Potential
	Transmembrane	483 – 503	21	Potential
	Transmembrane	555 – 575	21	Potential
	Transmembrane	604 – 624	21	Potential
	Region	1 – 491	491	COX1
	Region	492 – 773	282	COX2
Sites
	Metal binding	85	1	Iron (heme A axial ligand) By similarity
	Metal binding	264	1	Copper B By similarity
	Metal binding	268	1	Copper B By similarity
	Metal binding	314	1	Copper B By similarity
	Metal binding	315	1	Copper B By similarity
	Metal binding	400	1	Iron (heme A3 axial ligand) By similarity
	Metal binding	402	1	Iron (heme A axial ligand) By similarity
	Metal binding	709	1	Copper A By similarity
	Metal binding	744	1	Copper A By similarity
	Metal binding	748	1	Copper A By similarity
	Metal binding	752	1	Copper A By similarity
Amino acid modifications
	Cross-link	264 ↔ 268		1'-histidyl-3'-tyrosine (His-Tyr) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LCQ6-1 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: DBAB29E1527D1BD4	FASTA	773	86,778
	10 20 30 40 50 60 MKLLEIYDKQ LVEQEEGGFR AILTRYLNKW IFTVDHKLIG TMYITFSIFA GIIGTLLSLV 70 80 90 100 110 120 IPMELSTGNM LEGDSQQYNV IVTAHGLIMI FFVVMYCSMP AMLGGFANWF LPIMVGAPDV 130 140 150 160 170 180 AFPRLNNISL WLIVVSFGLL LTSSCVGIGA GTGWTVYPPL SMMEYHPGHA VDVGILSLHI 190 200 210 220 230 240 AGASSLVGAI NFLTTVFNMK IAGLSWPKVS LFVWSVVITA VLLVLSLPVL AGGLTMLITD 250 260 270 280 290 300 RNFETTFFDP IGGGDPILYQ HLFHPEVYIL ILPGFGIISI IISRYSNKGI FGVKGMISAM 310 320 330 340 350 360 SAIGFLGFLV WAYHHMYTVG LDVDTRAYFT AATMIIAIPT GIKIFSWLAT LWGGVIKITT 370 380 390 400 410 420 PMLFVIGFLV LFTIGGLTGV VLANGGLDIS LHDTYYVVAH FHYVLSMGAI FAIFAGYYYY 430 440 450 460 470 480 YAIMNSNRIL GIVRYNEQLG RIHFWTMFIG VNVTFFPMHF LGLAGMPRRI GDYPDAYIGW 490 500 510 520 530 540 NLIASYGSLI TAFGLLFFFV NIFTPYFKKK ALISKKFQRG AMILMGLDFS RDWQIGFQDP 550 560 570 580 590 600 ATPIMEGIID LHNYIFFYLI VVAVFIGWVM GRILWRFAYK WSYPTIGDIE IFKNFTAYNQ 610 620 630 640 650 660 IIHGTVIEIV WTLIPTVILY LIAIPSFTLL YAMDEIINPT VTIKIIGHQW YWSYEYGDNS 670 680 690 700 710 720 SNLVEFDSYM VYERDLNEGQ LRLLEVDNSM IVPVKTHIRL IITSGDVLHS WAVPSFGIKV 730 740 750 760 770 DAVPGRLNQI GLYVKREGTF YGQCSELCGV DHGFMPIKVE AVKVQEYLGR LYK

References

[1]	"Mitochondrial genome evolution in the social amoebae." Heidel A.J., Gloeckner G. Mol. Biol. Evol. 25:1440-1450(2008) [PubMed: 18413355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ336395 Genomic DNA. Translation: ABC60387.1.
RefSeq	YP_492636.1.
3D structure databases
SMR	Q2LCQ6. Positions 530-769.
ModBase	Search...
Genome annotation databases
GeneID	3912629.
Family and domain databases
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR000883. Cyt_c_oxidase_su1. IPR014222. Cyt_c_oxidase_su2. IPR015964. Cyt_c_oxidase_su2-like_TM_dom. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. [Graphical view]
Gene3D	G3DSA:1.20.210.10. COX1. 1 hit. G3DSA:1.10.287.90. COX2_TM. 1 hit. G3DSA:2.60.40.420. Cupredoxin. 1 hit.
PANTHER	PTHR10422. COX1. 1 hit.
Pfam	PF00115. COX1. 1 hit. PF00116. COX2. 1 hit. PF02790. COX2_TM. 1 hit. [Graphical view]
PRINTS	PR01165. CYCOXIDASEI.
TIGRFAMs	TIGR02866. CoxB. 1 hit.
PROSITE	PS50855. COX1. 1 hit. PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. PS50999. COX2_TM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX1_DICCI

Accession

Primary (citable) accession number: Q2LCQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	February 21, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)