Reviewed, UniProtKB/Swiss-Prot Q2LCP5 (NDUS1_DICCI)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-75kD
      Short name=CI-75kD

Gene names

Name:	nad11
Synonyms:	ndufs1

Encoded on

Mitochondrion

Organism

Dictyostelium citrinum (Slime mold)

Taxonomic identifier

361072 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 45 different subunits By similarity.
Subcellular location	Mitochondrion inner membraneBy similarity. Note= Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	NADH-ubiquinone oxidoreductase 75 kDa subunit		PRO_0000327378
Regions
	Domain	1 – 85	85	2Fe-2S ferredoxin-type
Sites
	Metal binding	38	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	49	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	52	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	66	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	101	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	105	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	108	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	114	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	153	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	156	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	159	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	204	1	Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LCP5-1 [UniParc]. Last modified April 8, 2008. Version 2. Checksum: FDC7CCB227832A0C	FASTA	688	79,605
	10 20 30 40 50 60 MLIRFKINEI ECEVDEEKED LTILQACTAN GIEIPRFCYH EKLTIAGNCR MCLVYVTNEE 70 80 90 100 110 120 KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN HPLDCPICDQ GGECDLQEQT 130 140 150 160 170 180 IAYGLDTGRF YIKKRAVEVK AFGRLIKGIM TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN 190 200 210 220 230 240 MEIGTYKKNV IIESELSGNI IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTVLTPIN 250 260 270 280 290 300 YQVKGGEIFR ILPRINDRLN EEWITDKVRF HYESYKIIEK MRKDTPSYKI QANKFIELSW 310 320 330 340 350 360 KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN GLLFKKFNYD 370 380 390 400 410 420 FRENYINSND LYNVDQNDLV LLCGINLRVE SPLLNIKLRN INFGDDEIET RKKIGIIGNK 430 440 450 460 470 480 FDWKQESEYL GSTVNSMLKV FEGRFPYCQQ IKKSKAPLIL VGSSLLSRIA ISLQEIRAAF 490 500 510 520 530 540 EIASNIKPEN VLLITQGANF GMALEEGIFK ENFSKGGNVL YSIDSNEYKV SKTINYIIYQ 550 560 570 580 590 600 GILNDTFENK IDLYLPSKHY FEDFESDREI YVNTFGQRSE IEKLRISKGN KIKENSMIGY 610 620 630 640 650 660 IQLMYLNKKE MNRKEKEQKE IKITYRGIKQ KEKRQVKINK NLTINNIIDN YYMTDINSRL 670 680 SKNLMITGQL RKEKKIMEAG SWKNKTCI

References

[1]	"Evolutionary trends found in social amoebae." Gloeckner G., Schaap P., Winckler T. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ336395 Genomic DNA. Translation: ABC60398.4.
RefSeq	YP_492647.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3912635.
Family and domain databases
InterPro	IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_DHase_75KDa_su_CS. IPR010228. NADH_quinone_OxRdtase_G. [Graphical view]
Pfam	PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. [Graphical view]
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_DICCI

Accession

Primary (citable) accession number: Q2LCP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	November 25, 2008
This is version 22 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)