Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2LCP5

- NDUS1_DICCI

UniProt

Q2LCP5 - NDUS1_DICCI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

nad11

Organism
Dictyostelium citrinum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi49 – 491Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi52 – 521Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi66 – 661Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi105 – 1051Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi108 – 1081Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi153 – 1531Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi156 – 1561Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi159 – 1591Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi204 – 2041Iron-sulfur 3 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
Gene namesi
Name:nad11
Synonyms:ndufs1
Encoded oniMitochondrion
OrganismiDictyostelium citrinum (Slime mold)
Taxonomic identifieri361072 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688NADH-ubiquinone oxidoreductase 75 kDa subunitPRO_0000327378Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ2LCP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 85852Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini223 – 279574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamiPF00384. Molybdopterin. 2 hits.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2LCP5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLIRFKINEI ECEVDEEKED LTILQACTAN GIEIPRFCYH EKLTIAGNCR
60 70 80 90 100
MCLVYVTNEE KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN
110 120 130 140 150
HPLDCPICDQ GGECDLQEQT IAYGLDTGRF YIKKRAVEVK AFGRLIKGIM
160 170 180 190 200
TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN MEIGTYKKNV IIESELSGNI
210 220 230 240 250
IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTVLTPIN YQVKGGEIFR
260 270 280 290 300
ILPRINDRLN EEWITDKVRF HYESYKIIEK MRKDTPSYKI QANKFIELSW
310 320 330 340 350
KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN
360 370 380 390 400
GLLFKKFNYD FRENYINSND LYNVDQNDLV LLCGINLRVE SPLLNIKLRN
410 420 430 440 450
INFGDDEIET RKKIGIIGNK FDWKQESEYL GSTVNSMLKV FEGRFPYCQQ
460 470 480 490 500
IKKSKAPLIL VGSSLLSRIA ISLQEIRAAF EIASNIKPEN VLLITQGANF
510 520 530 540 550
GMALEEGIFK ENFSKGGNVL YSIDSNEYKV SKTINYIIYQ GILNDTFENK
560 570 580 590 600
IDLYLPSKHY FEDFESDREI YVNTFGQRSE IEKLRISKGN KIKENSMIGY
610 620 630 640 650
IQLMYLNKKE MNRKEKEQKE IKITYRGIKQ KEKRQVKINK NLTINNIIDN
660 670 680
YYMTDINSRL SKNLMITGQL RKEKKIMEAG SWKNKTCI
Length:688
Mass (Da):79,605
Last modified:April 8, 2008 - v2
Checksum:iFDC7CCB227832A0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ336395 Genomic DNA. Translation: ABC60398.4.
RefSeqiYP_003579824.1. NC_007787.2.

Genome annotation databases

GeneIDi9086870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ336395 Genomic DNA. Translation: ABC60398.4 .
RefSeqi YP_003579824.1. NC_007787.2.

3D structure databases

ProteinModelPortali Q2LCP5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 9086870.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view ]
Pfami PF00384. Molybdopterin. 2 hits.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolutionary trends found in social amoebae."
    Gloeckner G., Schaap P., Winckler T.
    Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiNDUS1_DICCI
AccessioniPrimary (citable) accession number: Q2LCP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: November 26, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3