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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

nad11

Organism
Dictyostelium citrinum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi49Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi52Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi66Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi101Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi105Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi108Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi114Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi153Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi156Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi159Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi204Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
Gene namesi
Name:nad11
Synonyms:ndufs1
Encoded oniMitochondrion
OrganismiDictyostelium citrinum (Slime mold)
Taxonomic identifieri361072 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003273781 – 688NADH-ubiquinone oxidoreductase 75 kDa subunitAdd BLAST688

Proteomic databases

PRIDEiQ2LCP5

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ2LCP5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 852Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST85
Domaini85 – 1244Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini223 – 2794Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
PfamiView protein in Pfam
PF00384 Molybdopterin, 2 hits
PF10588 NADH-G_4Fe-4S_3, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q2LCP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIRFKINEI ECEVDEEKED LTILQACTAN GIEIPRFCYH EKLTIAGNCR
60 70 80 90 100
MCLVYVTNEE KLLAACGIPL DENFDDESIE TEIDEILKAR EGVMEFLLIN
110 120 130 140 150
HPLDCPICDQ GGECDLQEQT IAYGLDTGRF YIKKRAVEVK AFGRLIKGIM
160 170 180 190 200
TRCIHCTRCV RFLTEIAGVN ELGVLGRGYN MEIGTYKKNV IIESELSGNI
210 220 230 240 250
IDLCPVGALT SAVYAYKGRP WELKNIKGID IFDTVLTPIN YQVKGGEIFR
260 270 280 290 300
ILPRINDRLN EEWITDKVRF HYESYKIIEK MRKDTPSYKI QANKFIELSW
310 320 330 340 350
KTALKMVFKV LLNKKNKVDL IIGSKINSTN LRIYKELMNR LGSKNYITEN
360 370 380 390 400
GLLFKKFNYD FRENYINSND LYNVDQNDLV LLCGINLRVE SPLLNIKLRN
410 420 430 440 450
INFGDDEIET RKKIGIIGNK FDWKQESEYL GSTVNSMLKV FEGRFPYCQQ
460 470 480 490 500
IKKSKAPLIL VGSSLLSRIA ISLQEIRAAF EIASNIKPEN VLLITQGANF
510 520 530 540 550
GMALEEGIFK ENFSKGGNVL YSIDSNEYKV SKTINYIIYQ GILNDTFENK
560 570 580 590 600
IDLYLPSKHY FEDFESDREI YVNTFGQRSE IEKLRISKGN KIKENSMIGY
610 620 630 640 650
IQLMYLNKKE MNRKEKEQKE IKITYRGIKQ KEKRQVKINK NLTINNIIDN
660 670 680
YYMTDINSRL SKNLMITGQL RKEKKIMEAG SWKNKTCI
Length:688
Mass (Da):79,605
Last modified:April 8, 2008 - v2
Checksum:iFDC7CCB227832A0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ336395 Genomic DNA Translation: ABC60398.4
RefSeqiYP_003579824.1, NC_007787.2

Genome annotation databases

GeneIDi9086870

Similar proteinsi

Entry informationi

Entry nameiNDUS1_DICCI
AccessioniPrimary (citable) accession number: Q2LCP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 8, 2008
Last modified: May 23, 2018
This is version 65 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

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