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Q2LCM1

- CP21A_CANLU

UniProt

Q2LCM1 - CP21A_CANLU

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Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Canis lupus (Gray wolf)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

Catalytic activityi

A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi426 – 4261Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. steroid 21-monooxygenase activity Source: UniProtKB-EC
  4. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.99.10)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:CYP21
OrganismiCanis lupus (Gray wolf)
Taxonomic identifieri9612 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492Steroid 21-hydroxylasePRO_0000269709Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ2LCM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni340 – 35617Steroid-bindingBy similarityAdd
BLAST

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2LCM1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLLGVLLLT VLAGARLLWG KWKLRGLHLP PLVPGCLHLL QPDLPLHLLG
60 70 80 90 100
LTQKLGPIYR LRLGLQDVVV LNSKRTIEEA MVRKWVDFAG RPQTPSYKLV
110 120 130 140 150
SLHHQDLSLG DYSLLWKAHK KLTRSALLLG IRSSMEPLVE QLTQEFCERM
160 170 180 190 200
RAQAGTPVAI QKEFSLLTCA IICHLTFGDK EDTLVHTFHD CVQDLMRTWE
210 220 230 240 250
HWSIQMLDII PFLRFFPNPG LWRLKRALEN RDHIVEKQLR QHKESMVAGQ
260 270 280 290 300
WRDMTDYMLQ RVGRLRAEEG CGQLLEGHVH MSVVDLFIGG TETTATTLSW
310 320 330 340 350
AVAFLLHHPE IQQRLQEELD RELGPGASGS RIPYRDPTRL PLLSATVAEV
360 370 380 390 400
LRLRPVVPLA LPHCTTRPSS ISGYDIPEGM VVIPNLQGAH LDETVWERPQ
410 420 430 440 450
EFRPDRFLVP GASPRVLAFG CGARVCLGEP LARLELLVVL AQLLRAFTLM
460 470 480 490
PAAGTLPSLR PRARCGVNLS MQPFQVQLQP RGAGVLGRGQ HP
Length:492
Mass (Da):55,366
Last modified:February 21, 2006 - v1
Checksum:i8151F79FF0484A54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ336566 Genomic DNA. Translation: ABC67289.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ336566 Genomic DNA. Translation: ABC67289.1 .

3D structure databases

ProteinModelPortali Q2LCM1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG106944.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Phylogenetic analysis of a steroid 21-hydroxylase gene in some species of animals and a man."
    Kosowska B., Brzezinska K., Dobosz T., Moska M., Strzala T., Marszalek B., Schmidt K.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Muscle.

Entry informationi

Entry nameiCP21A_CANLU
AccessioniPrimary (citable) accession number: Q2LCM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: February 21, 2006
Last modified: November 26, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3