ID   ASHH2_ARATH             Reviewed;        1759 AA.
AC   Q2LAE1; O80663; Q56WW4;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   16-JUN-2009, entry version 33.
DE   RecName: Full=Histone-lysine N-methyltransferase ASHH2;
DE            EC=2.1.1.43;
DE   AltName: Full=H3-K4-HMTase;
DE   AltName: Full=Histone H3-K36 methyltransferase 8;
DE   AltName: Full=H3-K36-HMTase;
DE   AltName: Full=ASH1 homolog 2;
DE   AltName: Full=Protein EARLY FLOWERING IN SHORT DAYS;
DE   AltName: Full=Protein SET DOMAIN GROUP 8;
GN   Name=ASHH2; Synonyms=EFS, SDG8, SET8; OrderedLocusNames=At1g77300;
GN   ORFNames=T14N5.15;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=16299497; DOI=10.1038/ncb1329;
RA   Zhao Z., Yu Y., Meyer D., Wu C., Shen W.-H.;
RT   "Prevention of early flowering by expression of FLOWERING LOCUS C
RT   requires methylation of histone H3 K36.";
RL   Nat. Cell Biol. 7:1256-1260(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1299-1759 (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NOMENCLATURE.
RX   MEDLINE=21550130; PubMed=11691919; DOI=10.1093/nar/29.21.4319;
RA   Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K.,
RA   Assalkhou R., Schulz I., Reuter G., Aalen R.B.;
RT   "The Arabidopsis thaliana genome contains at least 29 active genes
RT   encoding SET domain proteins that can be assigned to four
RT   evolutionarily conserved classes.";
RL   Nucleic Acids Res. 29:4319-4333(2001).
RN   [5]
RP   FUNCTION.
RX   MEDLINE=99449572; PubMed=10518493;
RA   Soppe W.J.J., Bentsink L., Koornneef M.;
RT   "The early-flowering mutant efs is involved in the autonomous
RT   promotion pathway of Arabidopsis thaliana.";
RL   Development 126:4763-4770(1999).
RN   [6]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=16258034; DOI=10.1105/tpc.105.034645;
RA   Kim S.Y., He Y., Jacob Y., Noh Y.-S., Michaels S., Amasino R.;
RT   "Establishment of the vernalization-responsive, winter-annual habit in
RT   Arabidopsis requires a putative histone H3 methyl transferase.";
RL   Plant Cell 17:3301-3310(2005).
CC   -!- FUNCTION: Histone methyltransferase. Methylates 'Lys-4' and 'Lys-
CC       36' of histone H3. H3 'Lys-4' and 'Lys-36' methylations represent
CC       specific tags for epigenetic transcriptional activation. Regulates
CC       positively FLC transcription to prevent early flowering
CC       transition. Required for flowering transition in response to
CC       vernalization. Seems also to modulate several traits including
CC       floral organ size, root size and dormancy. Promotes apical
CC       dominance.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine =
CC       S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
CC   -!- SUBCELLULAR LOCATION: Nucleus (Probable). Centromere (Probable).
CC       Note=Associates with centromeric constitutive heterochromatin
CC       (Probable).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q2LAE1-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q2LAE1-2; Sequence=VSP_018133;
CC         Note=No experimental confirmation available;
CC   -!- TISSUE SPECIFICITY: Ubiquitous, with higher levels in young
CC       tissues, including shoot and root apex.
CC   -!- SIMILARITY: Belongs to the histone-lysine methyltransferase
CC       family. SET2 subfamily.
CC   -!- SIMILARITY: Contains 1 AWS domain.
CC   -!- SIMILARITY: Contains 1 CW-type zinc finger.
CC   -!- SIMILARITY: Contains 1 post-SET domain.
CC   -!- SIMILARITY: Contains 1 SET domain.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC34358.1; Type=Erroneous gene model prediction;
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DR   EMBL; DQ340869; ABC69038.1; -; mRNA.
DR   EMBL; AC004260; AAC34358.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AK221916; BAD94318.1; ALT_INIT; mRNA.
DR   IPI; IPI00542920; -.
DR   IPI; IPI00759360; -.
DR   PIR; T00458; T00458.
DR   HSSP; Q8X225; 1PEG.
DR   PRIDE; Q2LAE1; -.
DR   GenomeReviews; CT485782_GR; AT1G77300.
DR   TAIR; At1g77300; -.
DR   BRENDA; 2.1.1.43; 302.
DR   GermOnline; AT1G77300; Arabidopsis thaliana.
DR   GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0042800; F:histone methyltransferase activity (H3-K4 s...; IDA:TAIR.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016116; P:carotenoid metabolic process; IMP:TAIR.
DR   GO; GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
DR   GO; GO:0009910; P:negative regulation of flower development; IMP:TAIR.
DR   GO; GO:0031062; P:positive regulation of histone methylation; IDA:TAIR.
DR   GO; GO:0040029; P:regulation of gene expression, epigenetic; IMP:TAIR.
DR   GO; GO:0010223; P:secondary shoot formation; IMP:TAIR.
DR   InterPro; IPR006560; AWS.
DR   InterPro; IPR003616; Post-SET_Zn_bd.
DR   InterPro; IPR001214; SET.
DR   InterPro; IPR011124; Znf_CW.
DR   Pfam; PF00856; SET; 1.
DR   Pfam; PF07496; zf-CW; 1.
DR   SMART; SM00570; AWS; 1.
DR   SMART; SM00508; PostSET; 1.
DR   SMART; SM00317; SET; 1.
DR   PROSITE; PS51215; AWS; 1.
DR   PROSITE; PS50868; POST_SET; 1.
DR   PROSITE; PS50280; SET; 1.
DR   PROSITE; PS51050; ZF_CW; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Centromere; Chromatin regulator;
KW   Complete proteome; Metal-binding; Methyltransferase; Nucleus;
KW   S-adenosyl-L-methionine; Transferase; Zinc; Zinc-finger.
FT   CHAIN         1   1759       Histone-lysine N-methyltransferase ASHH2.
FT                                /FTId=PRO_0000233371.
FT   DOMAIN      974   1024       AWS.
FT   DOMAIN     1025   1147       SET.
FT   DOMAIN     1151   1167       Post-SET.
FT   ZN_FING     859    912       CW-type.
FT   VAR_SEQ    1447   1447       G -> GLQMLHNIMKQYRGDFKRIPIIRKLLKVLEYLATRK
FT                                ILALEHIIRRP (in isoform 2).
FT                                /FTId=VSP_018133.
FT   CONFLICT   1426   1426       I -> T (in Ref. 3; BAD94318).
SQ   SEQUENCE   1759 AA;  193228 MW;  5616BE25ECEF2155 CRC64;
     MDCKENGVGD ASGCNIDANS LASNLAMNTN EDFYEKLSSR GQNLDSVSSL EIPQTASSVN
     HTIEGQRKCF TEIEQMGYGN SNSQEDAGNT DDDLYVCYNA DDTQEQGVVS GELEQSQELI
     CDTDLLVNCN KLDDGKESQD TNVSLVSIFS GSMQEKEAPQ AKEDEGYGGT TLPIGGSGID
     TESTFVNDAP EQFESLETTK HIKPDEVESD GISYRFDDGG KEGRNGPSSD LDTGSSDDIS
     LSQSFSFPDS LLDSSVFGCS ATESYLEDAI DIEGNGTIVV SPSLAITEML NNDDGGLCSH
     DLNKITVTET INPDLKLVRE DRLDTDLSVM NEKMLKNHVG DSSSESAVAA LSMNNGMAAD
     LRAENFSQSS PIDEKTLDME ANSPITDSSL IWNFPLNFGS GGIEVCNPEN AVEPLRIVDD
     NGRIGGEVAS ASGSDFCEAG MSSSRRKARD GKQCKVVQTK TSARHLRKSS RKKQSERDIE
     SIFKCSKQKR SSLLKTSRSS EWGLPSKTTE IFLQSNNIPY DGPPHHEPQR SQGNLNNGEH
     NRSSHNGNVE GSNRNIQASS GSCLRLKVKF GKSGGQNPLN ITVSKVSGNS LPGNGIVKAG
     TCLELPGSAH FGEDKMQTVE TKEDLVEKSN PVEKVSYLQS SDSMRDKKYN QDAGGLCRKV
     GGDVLDDDPH LSSIRMVEEC ERATGTQSLD AETSPDSEVI NSVPDSIVNI EHKEGLHHGF
     FSTPEDVVKK NRVLEKEDEL RASKSPSENG SHLIPNAKKA KHPKSKSNGT KKGKSKFSES
     AKDGRKNESH EGVEQRKSLN TSMGRDDSDY PEVGRIESHK TTGALLDADI GKTSATYGTI
     SSDVTHGEMV VDVTIEDSYS TESAWVRCDD CFKWRRIPAS VVGSIDESSR WICMNNSDKR
     FADCSKSQEM SNEEINEELG IGQDEADAYD CDAAKRGKEK EQKSKRLTGK QKACFKAIKT
     NQFLHRNRKS QTIDEIMVCH CKPSPDGRLG CGEECLNRML NIECLQGTCP AGDLCSNQQF
     QKRKYVKFER FQSGKKGYGL RLLEDVREGQ FLIEYVGEVL DMQSYETRQK EYAFKGQKHF
     YFMTLNGNEV IDAGAKGNLG RFINHSCEPN CRTEKWMVNG EICVGIFSMQ DLKKGQELTF
     DYNYVRVFGA AAKKCYCGSS HCRGYIGGDP LNGDVIIQSD SDEEYPELVI LDDDESGEGI
     LGATSRTFTD DADEQMPQSF EKVNGYKDLA PDNTQTQSSV SVKLPEREIP PPLLQPTEVL
     KELSSGISIT AVQQEVPAEK KTKSTSPTSS SLSRMSPGGT NSDKTTKHGS GEDKKILPRP
     RPRMKTSRSS ESSKRDKGGI YPGVNKAQVI PVNKLQQQPI KSKGSEKVSP SIETFEGKLN
     ELLDAVGGIS KRRDSAKGYL KLLLLTAASR GTDEEGIYSN RDLSMILDAL LKTKSKSVLV
     DIINKNGPFA GMESFKDSVL SFTEHDDYTV HNIARSFRDR WIPKHFRKPW RINREERSES
     MRSPINRRFR ASQEPRYDHQ SPRPAEPAAS VTSSKAATPE TASVSEGYSE PNSGLPETNG
     RKRKSRWDQP SKTKEQRIMT ILSQQTDETN GNQDVQDDLP PGFSSPCTDV PDAITAQPQQ
     KFLSRLPVSY GIPLSIVHQF GSPGKEDPTT WSVAPGMPFY PFPPLPPVSH GEFFAKRNVR
     ACSSSMGNLT YSNEILPATP VTDSTAPTRK RELFSSDIGT TYFRQQKQSV PPWLRNNGGE
     KTANSPIPGN LTLEKKLNS
//