Reviewed, UniProtKB/Swiss-Prot Q2LAE1 (ASHH2_ARATH)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase ASHH2
    EC=2.1.1.43
Alternative name(s):
    H3-K4-HMTase
    Histone H3-K36 methyltransferase 8
    H3-K36-HMTase
    ASH1 homolog 2
    Protein EARLY FLOWERING IN SHORT DAYS
    Protein SET DOMAIN GROUP 8

Gene names

Name:	ASHH2
Synonyms:	EFS, SDG8, SET8
Ordered Locus Names:	At1g77300
ORF Names:	T14N5.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1759 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Histone methyltransferase. Methylates 'Lys-4' and 'Lys-36' of histone H3. H3 'Lys-4' and 'Lys-36' methylations represent specific tags for epigenetic transcriptional activation. Regulates positively FLC transcription to prevent early flowering transition. Required for flowering transition in response to vernalization. Seems also to modulate several traits including floral organ size, root size and dormancy. Promotes apical dominance. Ref.1 Ref.5 Ref.6
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subcellular location	Nucleus Probable. Centromere Probable. Note: Associates with centromeric constitutive heterochromatin Probable.
Tissue specificity	Ubiquitous, with higher levels in young tissues, including shoot and root apex. Ref.1 Ref.6
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 1 AWS domain. Contains 1 CW-type zinc finger. Contains 1 post-SET domain. Contains 1 SET domain.
Sequence caution	The sequence AAC34358.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Centromere Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carotenoid metabolic process Inferred from mutant phenotype. Source: TAIR chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of flower development Ref.5 Inferred from mutant phenotype. Source: TAIR positive regulation of histone methylation Ref.6 Inferred from direct assay. Source: TAIR regulation of gene expression, epigenetic Inferred from mutant phenotype. Source: TAIR secondary shoot formation Inferred from mutant phenotype. Source: TAIR
Cellular component	chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone methyltransferase activity (H3-K4 specific) Ref.6 Inferred from direct assay. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q2LAE1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q2LAE1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1447-1447: G → GLQMLHNIMKQYRGDFKRIPIIRKLLKVLEYLATRKILALEHIIRRP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1759	1759	Histone-lysine N-methyltransferase ASHH2		PRO_0000233371
Regions
	Domain	974 – 1024	51	AWS
	Domain	1025 – 1147	123	SET
	Domain	1151 – 1167	17	Post-SET
	Zinc finger	859 – 912	54	CW-type
Natural variations
	Alternative sequence	1447	1	G → GLQMLHNIMKQYRGDFKRIP IIRKLLKVLEYLATRKILAL EHIIRRP in isoform 2.		VSP_018133
Experimental info
	Sequence conflict	1426	1	I → T in BAD94318. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: 5616BE25ECEF2155	FASTA	1,759	193,228
	10 20 30 40 50 60 MDCKENGVGD ASGCNIDANS LASNLAMNTN EDFYEKLSSR GQNLDSVSSL EIPQTASSVN 70 80 90 100 110 120 HTIEGQRKCF TEIEQMGYGN SNSQEDAGNT DDDLYVCYNA DDTQEQGVVS GELEQSQELI 130 140 150 160 170 180 CDTDLLVNCN KLDDGKESQD TNVSLVSIFS GSMQEKEAPQ AKEDEGYGGT TLPIGGSGID 190 200 210 220 230 240 TESTFVNDAP EQFESLETTK HIKPDEVESD GISYRFDDGG KEGRNGPSSD LDTGSSDDIS 250 260 270 280 290 300 LSQSFSFPDS LLDSSVFGCS ATESYLEDAI DIEGNGTIVV SPSLAITEML NNDDGGLCSH 310 320 330 340 350 360 DLNKITVTET INPDLKLVRE DRLDTDLSVM NEKMLKNHVG DSSSESAVAA LSMNNGMAAD 370 380 390 400 410 420 LRAENFSQSS PIDEKTLDME ANSPITDSSL IWNFPLNFGS GGIEVCNPEN AVEPLRIVDD 430 440 450 460 470 480 NGRIGGEVAS ASGSDFCEAG MSSSRRKARD GKQCKVVQTK TSARHLRKSS RKKQSERDIE 490 500 510 520 530 540 SIFKCSKQKR SSLLKTSRSS EWGLPSKTTE IFLQSNNIPY DGPPHHEPQR SQGNLNNGEH 550 560 570 580 590 600 NRSSHNGNVE GSNRNIQASS GSCLRLKVKF GKSGGQNPLN ITVSKVSGNS LPGNGIVKAG 610 620 630 640 650 660 TCLELPGSAH FGEDKMQTVE TKEDLVEKSN PVEKVSYLQS SDSMRDKKYN QDAGGLCRKV 670 680 690 700 710 720 GGDVLDDDPH LSSIRMVEEC ERATGTQSLD AETSPDSEVI NSVPDSIVNI EHKEGLHHGF 730 740 750 760 770 780 FSTPEDVVKK NRVLEKEDEL RASKSPSENG SHLIPNAKKA KHPKSKSNGT KKGKSKFSES 790 800 810 820 830 840 AKDGRKNESH EGVEQRKSLN TSMGRDDSDY PEVGRIESHK TTGALLDADI GKTSATYGTI 850 860 870 880 890 900 SSDVTHGEMV VDVTIEDSYS TESAWVRCDD CFKWRRIPAS VVGSIDESSR WICMNNSDKR 910 920 930 940 950 960 FADCSKSQEM SNEEINEELG IGQDEADAYD CDAAKRGKEK EQKSKRLTGK QKACFKAIKT 970 980 990 1000 1010 1020 NQFLHRNRKS QTIDEIMVCH CKPSPDGRLG CGEECLNRML NIECLQGTCP AGDLCSNQQF 1030 1040 1050 1060 1070 1080 QKRKYVKFER FQSGKKGYGL RLLEDVREGQ FLIEYVGEVL DMQSYETRQK EYAFKGQKHF 1090 1100 1110 1120 1130 1140 YFMTLNGNEV IDAGAKGNLG RFINHSCEPN CRTEKWMVNG EICVGIFSMQ DLKKGQELTF 1150 1160 1170 1180 1190 1200 DYNYVRVFGA AAKKCYCGSS HCRGYIGGDP LNGDVIIQSD SDEEYPELVI LDDDESGEGI 1210 1220 1230 1240 1250 1260 LGATSRTFTD DADEQMPQSF EKVNGYKDLA PDNTQTQSSV SVKLPEREIP PPLLQPTEVL 1270 1280 1290 1300 1310 1320 KELSSGISIT AVQQEVPAEK KTKSTSPTSS SLSRMSPGGT NSDKTTKHGS GEDKKILPRP 1330 1340 1350 1360 1370 1380 RPRMKTSRSS ESSKRDKGGI YPGVNKAQVI PVNKLQQQPI KSKGSEKVSP SIETFEGKLN 1390 1400 1410 1420 1430 1440 ELLDAVGGIS KRRDSAKGYL KLLLLTAASR GTDEEGIYSN RDLSMILDAL LKTKSKSVLV 1450 1460 1470 1480 1490 1500 DIINKNGPFA GMESFKDSVL SFTEHDDYTV HNIARSFRDR WIPKHFRKPW RINREERSES 1510 1520 1530 1540 1550 1560 MRSPINRRFR ASQEPRYDHQ SPRPAEPAAS VTSSKAATPE TASVSEGYSE PNSGLPETNG 1570 1580 1590 1600 1610 1620 RKRKSRWDQP SKTKEQRIMT ILSQQTDETN GNQDVQDDLP PGFSSPCTDV PDAITAQPQQ 1630 1640 1650 1660 1670 1680 KFLSRLPVSY GIPLSIVHQF GSPGKEDPTT WSVAPGMPFY PFPPLPPVSH GEFFAKRNVR 1690 1700 1710 1720 1730 1740 ACSSSMGNLT YSNEILPATP VTDSTAPTRK RELFSSDIGT TYFRQQKQSV PPWLRNNGGE 1750 KTANSPIPGN LTLEKKLNS
	Isoform 2. Checksum: 79D63F4C931B15D0	FASTA	1,805	198,851
	10 20 30 40 50 60 MDCKENGVGD ASGCNIDANS LASNLAMNTN EDFYEKLSSR GQNLDSVSSL EIPQTASSVN 70 80 90 100 110 120 HTIEGQRKCF TEIEQMGYGN SNSQEDAGNT DDDLYVCYNA DDTQEQGVVS GELEQSQELI 130 140 150 160 170 180 CDTDLLVNCN KLDDGKESQD TNVSLVSIFS GSMQEKEAPQ AKEDEGYGGT TLPIGGSGID 190 200 210 220 230 240 TESTFVNDAP EQFESLETTK HIKPDEVESD GISYRFDDGG KEGRNGPSSD LDTGSSDDIS 250 260 270 280 290 300 LSQSFSFPDS LLDSSVFGCS ATESYLEDAI DIEGNGTIVV SPSLAITEML NNDDGGLCSH 310 320 330 340 350 360 DLNKITVTET INPDLKLVRE DRLDTDLSVM NEKMLKNHVG DSSSESAVAA LSMNNGMAAD 370 380 390 400 410 420 LRAENFSQSS PIDEKTLDME ANSPITDSSL IWNFPLNFGS GGIEVCNPEN AVEPLRIVDD 430 440 450 460 470 480 NGRIGGEVAS ASGSDFCEAG MSSSRRKARD GKQCKVVQTK TSARHLRKSS RKKQSERDIE 490 500 510 520 530 540 SIFKCSKQKR SSLLKTSRSS EWGLPSKTTE IFLQSNNIPY DGPPHHEPQR SQGNLNNGEH 550 560 570 580 590 600 NRSSHNGNVE GSNRNIQASS GSCLRLKVKF GKSGGQNPLN ITVSKVSGNS LPGNGIVKAG 610 620 630 640 650 660 TCLELPGSAH FGEDKMQTVE TKEDLVEKSN PVEKVSYLQS SDSMRDKKYN QDAGGLCRKV 670 680 690 700 710 720 GGDVLDDDPH LSSIRMVEEC ERATGTQSLD AETSPDSEVI NSVPDSIVNI EHKEGLHHGF 730 740 750 760 770 780 FSTPEDVVKK NRVLEKEDEL RASKSPSENG SHLIPNAKKA KHPKSKSNGT KKGKSKFSES 790 800 810 820 830 840 AKDGRKNESH EGVEQRKSLN TSMGRDDSDY PEVGRIESHK TTGALLDADI GKTSATYGTI 850 860 870 880 890 900 SSDVTHGEMV VDVTIEDSYS TESAWVRCDD CFKWRRIPAS VVGSIDESSR WICMNNSDKR 910 920 930 940 950 960 FADCSKSQEM SNEEINEELG IGQDEADAYD CDAAKRGKEK EQKSKRLTGK QKACFKAIKT 970 980 990 1000 1010 1020 NQFLHRNRKS QTIDEIMVCH CKPSPDGRLG CGEECLNRML NIECLQGTCP AGDLCSNQQF 1030 1040 1050 1060 1070 1080 QKRKYVKFER FQSGKKGYGL RLLEDVREGQ FLIEYVGEVL DMQSYETRQK EYAFKGQKHF 1090 1100 1110 1120 1130 1140 YFMTLNGNEV IDAGAKGNLG RFINHSCEPN CRTEKWMVNG EICVGIFSMQ DLKKGQELTF 1150 1160 1170 1180 1190 1200 DYNYVRVFGA AAKKCYCGSS HCRGYIGGDP LNGDVIIQSD SDEEYPELVI LDDDESGEGI 1210 1220 1230 1240 1250 1260 LGATSRTFTD DADEQMPQSF EKVNGYKDLA PDNTQTQSSV SVKLPEREIP PPLLQPTEVL 1270 1280 1290 1300 1310 1320 KELSSGISIT AVQQEVPAEK KTKSTSPTSS SLSRMSPGGT NSDKTTKHGS GEDKKILPRP 1330 1340 1350 1360 1370 1380 RPRMKTSRSS ESSKRDKGGI YPGVNKAQVI PVNKLQQQPI KSKGSEKVSP SIETFEGKLN 1390 1400 1410 1420 1430 1440 ELLDAVGGIS KRRDSAKGYL KLLLLTAASR GTDEEGIYSN RDLSMILDAL LKTKSKSVLV 1450 1460 1470 1480 1490 1500 DIINKNGLQM LHNIMKQYRG DFKRIPIIRK LLKVLEYLAT RKILALEHII RRPPFAGMES 1510 1520 1530 1540 1550 1560 FKDSVLSFTE HDDYTVHNIA RSFRDRWIPK HFRKPWRINR EERSESMRSP INRRFRASQE 1570 1580 1590 1600 1610 1620 PRYDHQSPRP AEPAASVTSS KAATPETASV SEGYSEPNSG LPETNGRKRK SRWDQPSKTK 1630 1640 1650 1660 1670 1680 EQRIMTILSQ QTDETNGNQD VQDDLPPGFS SPCTDVPDAI TAQPQQKFLS RLPVSYGIPL 1690 1700 1710 1720 1730 1740 SIVHQFGSPG KEDPTTWSVA PGMPFYPFPP LPPVSHGEFF AKRNVRACSS SMGNLTYSNE 1750 1760 1770 1780 1790 1800 ILPATPVTDS TAPTRKRELF SSDIGTTYFR QQKQSVPPWL RNNGGEKTAN SPIPGNLTLE KKLNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prevention of early flowering by expression of FLOWERING LOCUS C requires methylation of histone H3 K36." Zhao Z., Yu Y., Meyer D., Wu C., Shen W.-H. Nat. Cell Biol. 7:1256-1260(2005) [PubMed: 16299497] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1299-1759 (ISOFORM 2). Strain: cv. Columbia.
[4]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed: 11691919] [Abstract] Cited for: NOMENCLATURE.
[5]	"The early-flowering mutant efs is involved in the autonomous promotion pathway of Arabidopsis thaliana." Soppe W.J.J., Bentsink L., Koornneef M. Development 126:4763-4770(1999) [PubMed: 10518493] [Abstract] Cited for: FUNCTION.
[6]	"Establishment of the vernalization-responsive, winter-annual habit in Arabidopsis requires a putative histone H3 methyl transferase." Kim S.Y., He Y., Jacob Y., Noh Y.-S., Michaels S., Amasino R. Plant Cell 17:3301-3310(2005) [PubMed: 16258034] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ340869 mRNA. Translation: ABC69038.1. AC004260 Genomic DNA. Translation: AAC34358.1. Sequence problems. AK221916 mRNA. Translation: BAD94318.1. Different initiation.
IPI	IPI00542920. IPI00759360.
PIR	T00458.
3D structure databases
HSSP	HSSP built from PDB template 1PEG based on UniProtKB Q8X225.
ModBase	Search...
Proteomic databases
PRIDE	Q2LAE1.
Genome annotation databases
GenomeReviews	Gene locus AT1G77300 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g77300.
Enzyme and pathway databases
BRENDA	2.1.1.43. 302.
Gene expression databases
GermOnline	AT1G77300. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006560. AWS. IPR003616. Post-SET_Zn_bd. IPR001214. SET. IPR011124. Znf_CW. [Graphical view]
Pfam	PF00856. SET. 1 hit. PF07496. zf-CW. 1 hit. [Graphical view]
SMART	SM00570. AWS. 1 hit. SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51215. AWS. 1 hit. PS50868. POST_SET. 1 hit. PS50280. SET. 1 hit. PS51050. ZF_CW. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASHH2_ARATH

Accession

Primary (citable) accession number: Q2LAE1
Secondary accession number(s): O80663, Q56WW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	February 21, 2006
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)