Reviewed, UniProtKB/Swiss-Prot Q2LA60 (CP21A_FELCA)

Names and origin

Protein names

Recommended name:
    Steroid 21-hydroxylase
    EC=1.14.99.10
Alternative name(s):
    Cytochrome P450 21
    Cytochrome P450 XXI
    21-OHase
    Cytochrome P450-C21
    Cytochrome P-450c21

Gene names

Name:

CYP21

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.
Catalytic activity	A steroid + AH2 + O2 = a 21-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Domain	The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Lipid-binding Metal-binding Steroid-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 21-monooxygenase activity Inferred from electronic annotation. Source: EC steroid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Steroid 21-hydroxylase		PRO_0000269710
Regions
	Region	340 – 356	17	Steroid-binding By similarity
Sites
	Metal binding	426	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2LA60-1 [UniParc]. Last modified February 21, 2006. Version 1. Checksum: 3BFA53788469E510	FASTA	492	55,476
	10 20 30 40 50 60 MLLLGLLLLT ALAGARLLWN KWKYRSLHLP PLAPGFLHLL QPDLPIYLLG LTQKLGPVYR 70 80 90 100 110 120 LRLGLQDVVV LNSKRTIEEA LIRRWVDFAG RPQMPSYKLV SQHYQDLSLG DYSLLWKAHK 130 140 150 160 170 180 KLTRSALLLG IRNSMEPLVE QLTQEFCERM RAQAGTPVAI QKEFSFLTCS VICCLTFGDK 190 200 210 220 230 240 EDTLVHAFHD CVEDLMKSWE HWSIQVLDIV PFLRFFPNPG LRRLKQALEN RDRIVEKQLR 250 260 270 280 290 300 QHKDSMVAGQ WRDMTDYMLQ GMGKPKVEKG HGRLLEGHVH MSVVDLFIGG TETTATTLSW 310 320 330 340 350 360 AVAFLLHHPE IQQRLQEELD CELGPGASGS RVPLKDPSRL PLLTATIAEV LRLRPVVPLA 370 380 390 400 410 420 LPHRTTRHSS ILGYDIPEGT VVIPNLQGAH LDDTVWEQPH EFRPDRFLVP GASPRVLAFG 430 440 450 460 470 480 CGARVCLGEP LARLELFVVL ARLLHAFTLL PPTGPLPSLR PRSHCGINLT MQPFQVRLQP 490 RGAVAPGPSQ HQ

References

[1]	"Phylogenetic analysis of a steroid 21-hydroxylase gene in some species of animals and a man." Brzezinska K., Kosowska B., Dobosz T., Moska M., Strzala T., Marszalek B. Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Birman.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ341429 Genomic DNA. Translation: ABC69211.1.
3D structure databases
HSSP	HSSP built from PDB template 2F9Q based on UniProtKB P10635.
SMR	Q2LA60. Positions 43-481.
ModBase	Search...
Genome annotation databases
Ensembl	ENSFCAT00000004523; ENSFCAP00000004176; ENSFCAG00000004522; Felis catus. [Genome view]
Phylogenomic databases
eggNOG	maNOG17639.
HOVERGEN	Q2LA60.
Enzyme and pathway databases
BRENDA	1.14.99.10. 273949.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP21A_FELCA

Accession

Primary (citable) accession number: Q2LA60

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	February 21, 2006
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)