ID   CNPY1_DANRE             Reviewed;         187 AA.
AC   Q2L6L1; Q90ZG9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   27-MAR-2024, entry version 100.
DE   RecName: Full=Protein canopy-1;
DE   AltName: Full=Protein D121;
DE   Flags: Precursor;
GN   Name=cnpy1; Synonyms=d121; ORFNames=si:dkey-265a7.3;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INTERACTION WITH FGFR1,
RP   INDUCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 184-HIS--LEU-187, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16488878; DOI=10.1016/j.cub.2006.01.055;
RA   Hirate Y., Okamoto H.;
RT   "Canopy1, a novel regulator of FGF signaling around the midbrain-hindbrain
RT   boundary in zebrafish.";
RL   Curr. Biol. 16:421-427(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 101-187.
RA   Hirate Y., Mieda M., Harada T., Yamasu K., Okamoto H.;
RT   "A systematic search for the downstream target genes of the midbrain-MHB
RT   reciprocal inductive signaling by ordered differential display revealed the
RT   expression of ephrin-A3 in the posterior tectum of zebrafish embryos.";
RL   Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the maintenance of the midbrain-hindbrain
CC       boundary (MHB) organizer. Contributes to a positive-feedback loop of
CC       FGF signaling in the MHB, enabling the MHB to exert its role as an
CC       organizer for the tectal and cerebellar development.
CC       {ECO:0000269|PubMed:16488878}.
CC   -!- SUBUNIT: Homodimer. Interacts with fgfr1.
CC       {ECO:0000269|PubMed:16488878}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC       {ECO:0000269|PubMed:16488878}.
CC   -!- DEVELOPMENTAL STAGE: Expressed maternally from early cleavage stages
CC       until the shield stage. Expressed zygotically in the polster at the bud
CC       stage. Expressed in the midbrain-hindbrain boundary (MHB) at 12 hours
CC       post-fertilization (hpf). Expressed in the tail bud and the mesenchymal
CC       cells of the caudal fin primordia at 26 hpf.
CC       {ECO:0000269|PubMed:16488878}.
CC   -!- INDUCTION: By the fibroblast growth factor fgf8 in the MHB.
CC       {ECO:0000269|PubMed:16488878}.
CC   -!- SIMILARITY: Belongs to the canopy family. {ECO:0000305}.
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DR   EMBL; AB201383; BAE78824.1; -; mRNA.
DR   EMBL; BX510360; CAM46989.1; -; Genomic_DNA.
DR   EMBL; BC122425; AAI22426.1; -; mRNA.
DR   EMBL; AB055671; BAB62803.1; -; mRNA.
DR   RefSeq; NP_001034586.1; NM_001039497.2.
DR   AlphaFoldDB; Q2L6L1; -.
DR   STRING; 7955.ENSDARP00000009432; -.
DR   PaxDb; 7955-ENSDARP00000009432; -.
DR   GeneID; 402819; -.
DR   KEGG; dre:402819; -.
DR   AGR; ZFIN:ZDB-GENE-060315-3; -.
DR   CTD; 285888; -.
DR   ZFIN; ZDB-GENE-060315-3; cnpy1.
DR   eggNOG; KOG3782; Eukaryota.
DR   HOGENOM; CLU_095726_2_1_1; -.
DR   InParanoid; Q2L6L1; -.
DR   OMA; ASANHTE; -.
DR   OrthoDB; 2878740at2759; -.
DR   PhylomeDB; Q2L6L1; -.
DR   TreeFam; TF318578; -.
DR   PRO; PR:Q2L6L1; -.
DR   Proteomes; UP000000437; Chromosome 2.
DR   Bgee; ENSDARG00000003757; Expressed in testis and 30 other cell types or tissues.
DR   ExpressionAtlas; Q2L6L1; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:ZFIN.
DR   GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR   GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IGI:ZFIN.
DR   GO; GO:0070121; P:Kupffer's vesicle development; IMP:ZFIN.
DR   GO; GO:0030917; P:midbrain-hindbrain boundary development; IMP:ZFIN.
DR   GO; GO:0040036; P:regulation of fibroblast growth factor receptor signaling pathway; IMP:ZFIN.
DR   InterPro; IPR042415; CNPY.
DR   InterPro; IPR021852; DUF3456.
DR   PANTHER; PTHR13341:SF4; CANOPY FGF-SIGNALING REGULATOR 1; 1.
DR   PANTHER; PTHR13341; MIR-INTERACTING SAPOSIN-LIKE PROTEIN; 1.
DR   Pfam; PF11938; DUF3456; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Disulfide bond; Endoplasmic reticulum;
KW   Reference proteome; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..187
FT                   /note="Protein canopy-1"
FT                   /id="PRO_0000314013"
FT   DOMAIN          28..181
FT                   /note="Saposin B-type"
FT   MOTIF           184..187
FT                   /note="Prevents secretion from ER"
FT                   /evidence="ECO:0000255"
FT   DISULFID        32..177
FT                   /evidence="ECO:0000250"
FT   DISULFID        35..170
FT                   /evidence="ECO:0000250"
FT   DISULFID        90..143
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         184..187
FT                   /note="HSEL->KDEL: Does not abolish endoplasmic reticulum
FT                   localization."
FT                   /evidence="ECO:0000269|PubMed:16488878"
FT   MUTAGEN         184..187
FT                   /note="Missing: Abolishes endoplasmic reticulum
FT                   localization."
FT                   /evidence="ECO:0000269|PubMed:16488878"
SQ   SEQUENCE   187 AA;  20973 MW;  BA9147D3A4BB47E0 CRC64;
     MSPWIKHICL VLVAAFMLVK TTESKKDEAL YCSACMAIAD EINYSISQTD PKKMIHVGGF
     RLKPDGSLTD KKVPLARSET YLTELLEEVC KSMSDYALYE NPDTKEKSYK RFAPRDNDGG
     NFPDFKNFKF DGPESSSALK FACESIVEEL EDDIISLFAS DSDHVAKTLC SEVSDHCKSS
     VFQHSEL
//