Q2L6L1 (CNPY1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein canopy-1

Alternative name(s):
Protein D121

Gene names

Name:	cnpy1
Synonyms:	d121
ORF Names:	si:dkey-265a7.3

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the maintenance of the midbrain-hindbrain boundary (MHB) organizer. Contributes to a positive-feedback loop of FGF signaling in the MHB, enabling the MHB to exert its role as an organizer for the tectal and cerebellar development. Ref.1
Subunit structure	Homodimer. Interacts with fgfr1. Ref.1
Subcellular location	Endoplasmic reticulum Ref.1.
Developmental stage	Expressed maternally from early cleavage stages until the shield stage. Expressed zygotically in the polster at the bud stage. Expressed in the midbrain-hindbrain boundary (MHB) at 12 hours post-fertilization (hpf). Expressed in the tail bud and the mesenchymal cells of the caudal fin primordia at 26 hpf. Ref.1
Induction	By the fibroblast growth factor fgf8 in the MHB. Ref.1
Sequence similarities	Belongs to the canopy family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Developmental protein
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Kupffer's vesicle development Inferred from mutant phenotype PubMed 21628557. Source: ZFIN determination of left/right symmetry Inferred from mutant phenotype PubMed 21628557. Source: ZFIN fibroblast growth factor receptor signaling pathway Inferred from genetic interaction Ref.1. Source: ZFIN midbrain-hindbrain boundary development Inferred from mutant phenotype Ref.1. Source: ZFIN regulation of fibroblast growth factor receptor signaling pathway Inferred from mutant phenotype PubMed 21628557. Source: ZFIN
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.1. Source: ZFIN
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 187	163	Protein canopy-1		PRO_0000314013
Regions
	Domain	28 – 181	154	Saposin B-type
	Motif	184 – 187	4	Prevents secretion from ER Potential
Amino acid modifications
	Disulfide bond	32 ↔ 177		By similarity
	Disulfide bond	35 ↔ 170		By similarity
	Disulfide bond	90 ↔ 143		By similarity
Experimental info
	Mutagenesis	184 – 187	4	HSEL → KDEL: Does not abolish endoplasmic reticulum localization. Ref.1
	Mutagenesis	184 – 187	4	Missing: Abolishes endoplasmic reticulum localization. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q2L6L1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: BA9147D3A4BB47E0	FASTA	187	20,973
	10 20 30 40 50 60 MSPWIKHICL VLVAAFMLVK TTESKKDEAL YCSACMAIAD EINYSISQTD PKKMIHVGGF 70 80 90 100 110 120 RLKPDGSLTD KKVPLARSET YLTELLEEVC KSMSDYALYE NPDTKEKSYK RFAPRDNDGG 130 140 150 160 170 180 NFPDFKNFKF DGPESSSALK FACESIVEEL EDDIISLFAS DSDHVAKTLC SEVSDHCKSS VFQHSEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Canopy1, a novel regulator of FGF signaling around the midbrain-hindbrain boundary in zebrafish." Hirate Y., Okamoto H. Curr. Biol. 16:421-427(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INTERACTION WITH FGFR1, INDUCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 184-HIS--LEU-187, DEVELOPMENTAL STAGE.
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[4]	"A systematic search for the downstream target genes of the midbrain-MHB reciprocal inductive signaling by ordered differential display revealed the expression of ephrin-A3 in the posterior tectum of zebrafish embryos." Hirate Y., Mieda M., Harada T., Yamasu K., Okamoto H. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 101-187.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB201383 mRNA. Translation: BAE78824.1. BX510360 Genomic DNA. Translation: CAM46989.1. BC122425 mRNA. Translation: AAI22426.1. AB055671 mRNA. Translation: BAB62803.1.
IPI	IPI00513161.
RefSeq	NP_001034586.1. NM_001039497.2.
UniGene	Dr.107959.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000009432.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000020792; ENSDARP00000009432; ENSDARG00000003757.
GeneID	402819.
KEGG	dre:402819.
Organism-specific databases
CTD	285888.
ZFIN	ZDB-GENE-060315-3. cnpy1.
Phylogenomic databases
eggNOG	NOG280279.
GeneTree	ENSGT00390000009749.
HOGENOM	HOG000233996.
HOVERGEN	HBG052514.
OMA	DPKKTIN.
OrthoDB	EOG4WWRKW.
Gene expression databases
Bgee	Q2L6L1.
Family and domain databases
InterPro	IPR021852. DUF3456. [Graphical view]
Pfam	PF11938. DUF3456. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. False negative. PS50015. SAP_B. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816648.

Entry information

Entry name

CNPY1_DANRE

Accession

Primary (citable) accession number: Q2L6L1
Secondary accession number(s): Q90ZG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program