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Q2L6L1 (CNPY1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein canopy-1
Alternative name(s):
Protein D121
Gene names
Name:cnpy1
Synonyms:d121
ORF Names:si:dkey-265a7.3
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the maintenance of the midbrain-hindbrain boundary (MHB) organizer. Contributes to a positive-feedback loop of FGF signaling in the MHB, enabling the MHB to exert its role as an organizer for the tectal and cerebellar development. Ref.1

Subunit structure

Homodimer. Interacts with fgfr1. Ref.1

Subcellular location

Endoplasmic reticulum Ref.1.

Developmental stage

Expressed maternally from early cleavage stages until the shield stage. Expressed zygotically in the polster at the bud stage. Expressed in the midbrain-hindbrain boundary (MHB) at 12 hours post-fertilization (hpf). Expressed in the tail bud and the mesenchymal cells of the caudal fin primordia at 26 hpf. Ref.1

Induction

By the fibroblast growth factor fgf8 in the MHB. Ref.1

Sequence similarities

Belongs to the canopy family.

Contains 1 saposin B-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 187163Protein canopy-1
PRO_0000314013

Regions

Domain28 – 181154Saposin B-type
Motif184 – 1874Prevents secretion from ER Potential

Amino acid modifications

Disulfide bond32 ↔ 177 By similarity
Disulfide bond35 ↔ 170 By similarity
Disulfide bond90 ↔ 143 By similarity

Experimental info

Mutagenesis184 – 1874HSEL → KDEL: Does not abolish endoplasmic reticulum localization. Ref.1
Mutagenesis184 – 1874Missing: Abolishes endoplasmic reticulum localization. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q2L6L1 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: BA9147D3A4BB47E0

FASTA18720,973
        10         20         30         40         50         60 
MSPWIKHICL VLVAAFMLVK TTESKKDEAL YCSACMAIAD EINYSISQTD PKKMIHVGGF 

        70         80         90        100        110        120 
RLKPDGSLTD KKVPLARSET YLTELLEEVC KSMSDYALYE NPDTKEKSYK RFAPRDNDGG 

       130        140        150        160        170        180 
NFPDFKNFKF DGPESSSALK FACESIVEEL EDDIISLFAS DSDHVAKTLC SEVSDHCKSS 


VFQHSEL 

« Hide

References

« Hide 'large scale' references
[1]"Canopy1, a novel regulator of FGF signaling around the midbrain-hindbrain boundary in zebrafish."
Hirate Y., Okamoto H.
Curr. Biol. 16:421-427(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INTERACTION WITH FGFR1, INDUCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 184-HIS--LEU-187, DEVELOPMENTAL STAGE.
[2]"The zebrafish reference genome sequence and its relationship to the human genome."
Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J. expand/collapse author list , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tuebingen.
[3]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[4]"A systematic search for the downstream target genes of the midbrain-MHB reciprocal inductive signaling by ordered differential display revealed the expression of ephrin-A3 in the posterior tectum of zebrafish embryos."
Hirate Y., Mieda M., Harada T., Yamasu K., Okamoto H.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 101-187.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB201383 mRNA. Translation: BAE78824.1.
BX510360 Genomic DNA. Translation: CAM46989.1.
BC122425 mRNA. Translation: AAI22426.1.
AB055671 mRNA. Translation: BAB62803.1.
RefSeqNP_001034586.1. NM_001039497.2.
UniGeneDr.107959.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000009432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000020792; ENSDARP00000009432; ENSDARG00000003757.
GeneID402819.
KEGGdre:402819.

Organism-specific databases

CTD285888.
ZFINZDB-GENE-060315-3. cnpy1.

Phylogenomic databases

eggNOGNOG280279.
GeneTreeENSGT00390000009749.
HOGENOMHOG000233996.
HOVERGENHBG052514.
OMACEDISKH.
OrthoDBEOG7XDBHB.
PhylomeDBQ2L6L1.
TreeFamTF318578.

Gene expression databases

BgeeQ2L6L1.

Family and domain databases

InterProIPR021852. DUF3456.
[Graphical view]
PfamPF11938. DUF3456. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816648.
PROQ2L6L1.

Entry information

Entry nameCNPY1_DANRE
AccessionPrimary (citable) accession number: Q2L6L1
Secondary accession number(s): Q90ZG9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families