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Q2L2K5

- SYI_BORA1

UniProt

Q2L2K5 - SYI_BORA1

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bordetella avium (strain 197N)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (07 Mar 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
    Binding sitei626 – 6261ATPUniRule annotation
    Metal bindingi916 – 9161ZincUniRule annotation
    Metal bindingi919 – 9191ZincUniRule annotation
    Metal bindingi936 – 9361ZincUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAVI360910:GCKI-1435-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BAV1411
    OrganismiBordetella avium (strain 197N)
    Taxonomic identifieri360910 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
    ProteomesiUP000001977: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Isoleucine--tRNA ligasePRO_1000022043Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi360910.BAV1411.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2L2K5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi623 – 6275"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2L2K5-1 [UniParc]FASTAAdd to Basket

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    MDYKKTLNLP DTSFPMRGDL AKREPGWVQQ WEENRVYQAI RAASKGRPLF    50
    ILHDGPPYAN GDIHIGHAVN KILKDIIVKS RSMAGYDAPY VPGWDCHGMP 100
    IEIQVEKKFG KNLPVTEVHA KARAYALEQL DRQRQDFKRL GVLGDWDNPY 150
    LTMNFSNEAD EIRVLARILE KGYVFRGLKP VNWCFDCGSA LAEAEVEYAD 200
    RVDPAIDVAF PFTDKAALAG AFGLDSVDDG AIVIWTTTPW TIPSNQALNV 250
    HPEIEYALVR VSPTPVHGPL VLIAKERVEA CLKTWGLEGE IIATAPGQAL 300
    DGLRFAHPLA RAAEGYDRTS PIYLGDYVTL DTGTGVVHSA PAYGIEDFVS 350
    CKNHGLADAD ILSPVMGDGK YIATLPLFGG LSIWDANPKI VEALKLAGSL 400
    MHVQKLSHSY MHCWRHKSPV IYRATSQWFA GMDVTPEGGG QTLRESALAG 450
    IEATTFYPAW GRARLQAMIA NRPDWTLSRQ RQWGVPMAFF VHKETGALHP 500
    RTVELLEEVA KRVEKSGIEA WQSLDPRELL GDEADSYEKN RDTLDVWFDS 550
    GSTHATVLGG KDHALHGSHG EQLAWPADLY LEGSDQHRGW FHSSLLTGCM 600
    LYGQPPYKGL LTHGFVVDGQ GRKMSKSVGN VIAPQKVSDS LGAEILRLWV 650
    ASTDYSGELS ISDEILKRVV EGYRRIRNTL RFLLANVADF DGVNQAVPYG 700
    DLLEIDRYAL VMTAQMQAEV QAHYQSYDFH PAVSRLQTFC SEDLGAFYLD 750
    ILKDRLYTNA PGSHARRSAQ TALLDITQTL VKLMAPILSF TAEEAWKVLA 800
    DSALKHQADA ARLTIFTEVY HTLPPYADAD ALAGRWGRLR AIRADVLRKL 850
    EDVRGEGLIG SSLQAEVDIY ADGEDLALLS ALGDDLRFVL IVSRATVHAR 900
    AGELAIEIAP SAHKKCERCW HWRADVGQDA DHPEICGRCV SNLFGAGESR 950
    AKA 953
    Length:953
    Mass (Da):105,398
    Last modified:March 7, 2006 - v1
    Checksum:i65C8B60E800D7C55
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM167904 Genomic DNA. Translation: CAJ49020.1.
    RefSeqiYP_785933.1. NC_010645.1.

    Genome annotation databases

    GeneIDi6266797.
    KEGGibav:BAV1411.
    PATRICi21129016. VBIBorAvi43433_1431.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM167904 Genomic DNA. Translation: CAJ49020.1 .
    RefSeqi YP_785933.1. NC_010645.1.

    3D structure databases

    ProteinModelPortali Q2L2K5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 360910.BAV1411.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6266797.
    KEGGi bav:BAV1411.
    PATRICi 21129016. VBIBorAvi43433_1431.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BAVI360910:GCKI-1435-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction."
      Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M.
      J. Bacteriol. 188:6002-6015(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 197N.

    Entry informationi

    Entry nameiSYI_BORA1
    AccessioniPrimary (citable) accession number: Q2L2K5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 7, 2006
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3