Reviewed, UniProtKB/Swiss-Prot Q2L2K5 (SYI_BORA1)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	BAV1411

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	953 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_02002
Subunit structure	Monomer By similarity. HAMAP MF_02002
Subcellular location	Cytoplasm By similarity HAMAP MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 953	953	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_1000022043
Regions
	Motif	57 – 67	11	"HIGH" region HAMAP MF_02002
	Motif	623 – 627	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	916	1	Zinc By similarity
	Metal binding	919	1	Zinc By similarity
	Metal binding	936	1	Zinc By similarity
	Metal binding	939	1	Zinc By similarity
	Binding site	582	1	Aminoacyl-adenylate By similarity
	Binding site	626	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2L2K5-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 65C8B60E800D7C55	FASTA	953	105,398
	10 20 30 40 50 60 MDYKKTLNLP DTSFPMRGDL AKREPGWVQQ WEENRVYQAI RAASKGRPLF ILHDGPPYAN 70 80 90 100 110 120 GDIHIGHAVN KILKDIIVKS RSMAGYDAPY VPGWDCHGMP IEIQVEKKFG KNLPVTEVHA 130 140 150 160 170 180 KARAYALEQL DRQRQDFKRL GVLGDWDNPY LTMNFSNEAD EIRVLARILE KGYVFRGLKP 190 200 210 220 230 240 VNWCFDCGSA LAEAEVEYAD RVDPAIDVAF PFTDKAALAG AFGLDSVDDG AIVIWTTTPW 250 260 270 280 290 300 TIPSNQALNV HPEIEYALVR VSPTPVHGPL VLIAKERVEA CLKTWGLEGE IIATAPGQAL 310 320 330 340 350 360 DGLRFAHPLA RAAEGYDRTS PIYLGDYVTL DTGTGVVHSA PAYGIEDFVS CKNHGLADAD 370 380 390 400 410 420 ILSPVMGDGK YIATLPLFGG LSIWDANPKI VEALKLAGSL MHVQKLSHSY MHCWRHKSPV 430 440 450 460 470 480 IYRATSQWFA GMDVTPEGGG QTLRESALAG IEATTFYPAW GRARLQAMIA NRPDWTLSRQ 490 500 510 520 530 540 RQWGVPMAFF VHKETGALHP RTVELLEEVA KRVEKSGIEA WQSLDPRELL GDEADSYEKN 550 560 570 580 590 600 RDTLDVWFDS GSTHATVLGG KDHALHGSHG EQLAWPADLY LEGSDQHRGW FHSSLLTGCM 610 620 630 640 650 660 LYGQPPYKGL LTHGFVVDGQ GRKMSKSVGN VIAPQKVSDS LGAEILRLWV ASTDYSGELS 670 680 690 700 710 720 ISDEILKRVV EGYRRIRNTL RFLLANVADF DGVNQAVPYG DLLEIDRYAL VMTAQMQAEV 730 740 750 760 770 780 QAHYQSYDFH PAVSRLQTFC SEDLGAFYLD ILKDRLYTNA PGSHARRSAQ TALLDITQTL 790 800 810 820 830 840 VKLMAPILSF TAEEAWKVLA DSALKHQADA ARLTIFTEVY HTLPPYADAD ALAGRWGRLR 850 860 870 880 890 900 AIRADVLRKL EDVRGEGLIG SSLQAEVDIY ADGEDLALLS ALGDDLRFVL IVSRATVHAR 910 920 930 940 950 AGELAIEIAP SAHKKCERCW HWRADVGQDA DHPEICGRCV SNLFGAGESR AKA

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM167904 Genomic DNA. Translation: CAJ49020.1.
RefSeq	YP_785933.1.
3D structure databases
SMR	Q2L2K5. Positions 1-943.
ModBase	Search...
Genome annotation databases
GeneID	6266797.
GenomeReviews	Gene locus BAV1411 in contig AM167904_GR.
KEGG	bav:BAV1411.
NMPDR	fig|521.1.peg.474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG577712.
OMA	KQVLTHG.
Family and domain databases
HAMAP	MF_02002. Ile_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BORA1

Accession

Primary (citable) accession number: Q2L2K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)