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Q2L2K5

- SYI_BORA1

UniProt

Q2L2K5 - SYI_BORA1

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BAV1411
Organism
Bordetella avium (strain 197N)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylate By similarity
Binding sitei626 – 6261ATP By similarity
Metal bindingi916 – 9161Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBAVI360910:GCKI-1435-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BAV1411
OrganismiBordetella avium (strain 197N)
Taxonomic identifieri360910 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
ProteomesiUP000001977: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022043Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi360910.BAV1411.

Structurei

3D structure databases

ProteinModelPortaliQ2L2K5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi623 – 6275"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2L2K5-1 [UniParc]FASTAAdd to Basket

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MDYKKTLNLP DTSFPMRGDL AKREPGWVQQ WEENRVYQAI RAASKGRPLF    50
ILHDGPPYAN GDIHIGHAVN KILKDIIVKS RSMAGYDAPY VPGWDCHGMP 100
IEIQVEKKFG KNLPVTEVHA KARAYALEQL DRQRQDFKRL GVLGDWDNPY 150
LTMNFSNEAD EIRVLARILE KGYVFRGLKP VNWCFDCGSA LAEAEVEYAD 200
RVDPAIDVAF PFTDKAALAG AFGLDSVDDG AIVIWTTTPW TIPSNQALNV 250
HPEIEYALVR VSPTPVHGPL VLIAKERVEA CLKTWGLEGE IIATAPGQAL 300
DGLRFAHPLA RAAEGYDRTS PIYLGDYVTL DTGTGVVHSA PAYGIEDFVS 350
CKNHGLADAD ILSPVMGDGK YIATLPLFGG LSIWDANPKI VEALKLAGSL 400
MHVQKLSHSY MHCWRHKSPV IYRATSQWFA GMDVTPEGGG QTLRESALAG 450
IEATTFYPAW GRARLQAMIA NRPDWTLSRQ RQWGVPMAFF VHKETGALHP 500
RTVELLEEVA KRVEKSGIEA WQSLDPRELL GDEADSYEKN RDTLDVWFDS 550
GSTHATVLGG KDHALHGSHG EQLAWPADLY LEGSDQHRGW FHSSLLTGCM 600
LYGQPPYKGL LTHGFVVDGQ GRKMSKSVGN VIAPQKVSDS LGAEILRLWV 650
ASTDYSGELS ISDEILKRVV EGYRRIRNTL RFLLANVADF DGVNQAVPYG 700
DLLEIDRYAL VMTAQMQAEV QAHYQSYDFH PAVSRLQTFC SEDLGAFYLD 750
ILKDRLYTNA PGSHARRSAQ TALLDITQTL VKLMAPILSF TAEEAWKVLA 800
DSALKHQADA ARLTIFTEVY HTLPPYADAD ALAGRWGRLR AIRADVLRKL 850
EDVRGEGLIG SSLQAEVDIY ADGEDLALLS ALGDDLRFVL IVSRATVHAR 900
AGELAIEIAP SAHKKCERCW HWRADVGQDA DHPEICGRCV SNLFGAGESR 950
AKA 953
Length:953
Mass (Da):105,398
Last modified:March 7, 2006 - v1
Checksum:i65C8B60E800D7C55
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM167904 Genomic DNA. Translation: CAJ49020.1.
RefSeqiYP_785933.1. NC_010645.1.

Genome annotation databases

GeneIDi6266797.
KEGGibav:BAV1411.
PATRICi21129016. VBIBorAvi43433_1431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM167904 Genomic DNA. Translation: CAJ49020.1 .
RefSeqi YP_785933.1. NC_010645.1.

3D structure databases

ProteinModelPortali Q2L2K5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 360910.BAV1411.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 6266797.
KEGGi bav:BAV1411.
PATRICi 21129016. VBIBorAvi43433_1431.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BAVI360910:GCKI-1435-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction."
    Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M.
    J. Bacteriol. 188:6002-6015(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 197N.

Entry informationi

Entry nameiSYI_BORA1
AccessioniPrimary (citable) accession number: Q2L2K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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