Reviewed, UniProtKB/Swiss-Prot Q2L1L0 (PCKG_BORA1)

Names and origin

Protein names

Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]
      Short name=PEP carboxykinase
      Short name=PEPCK
    EC=4.1.1.32
Alternative name(s):
    Phosphoenolpyruvate carboxylase

Gene names

Name:	pckG
Ordered Locus Names:	BAV1626

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.
Catalytic activity	GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452
Cofactor	Binds 1 manganese ion per subunit By similarity.
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	GTP-binding Manganese Metal-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (GTP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452		PRO_1000060287
Regions
	Nucleotide binding	276 – 281	6	GTP By similarity
	Nucleotide binding	526 – 529	4	GTP By similarity
	Region	396 – 398	3	Substrate binding By similarity
Sites
	Active site	277	1	By similarity
	Metal binding	233	1	Manganese By similarity
	Metal binding	253	1	Manganese By similarity
	Metal binding	302	1	Manganese By similarity
	Binding site	85	1	Substrate By similarity
	Binding site	226	1	Substrate; via amide nitrogen By similarity
	Binding site	233	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity
	Binding site	398	1	GTP By similarity
	Binding site	429	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2L1L0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 70C191E3B587F43F	FASTA	621	67,910
	10 20 30 40 50 60 MTQAAEATLP PLNVPSYVKH ARLIDWVQGV VALTKPARVV WCDGSQEEAD RLCEQMVQAG 70 80 90 100 110 120 TMRRLNPAKR PNSYLAWSDP SDVARVEDRT FICSQREEDA GPTNNWAAPA EMRNTLQGLF 130 140 150 160 170 180 DGAMRGRTLY VVPFSMGPLG SPIAHIGVEL SDSPYVAVNM RIMTRMGRAV WDVLGADGEF 190 200 210 220 230 240 VPCVHSVGMP LAEGQADVAW PCNPVKYIVH YPETREIWSF GSGYGGNALL GKKCFALRIA 250 260 270 280 290 300 STMGRDEGWL AEHMLILGVT TPKGRKFHVA AAFPSACGKT NFAMLIPPSG MDGWKVSTIG 310 320 330 340 350 360 DDIAWIKPGQ DGRLRAINPE AGYFGVAPGT SEKTNPNAMA TLKANVIFTN VALTDDGDVW 370 380 390 400 410 420 WEGMTDTPPA HLIDWQGKDW TPEIARETGR KAAHPNARFT APASQCPSID PEWENPQGVA 430 440 450 460 470 480 IDAFIFGGRR STTIPLVTEA RDWVQGVYMA ATMGSETTAA AVGQQGVVRR DPFAMLPFCG 490 500 510 520 530 540 YNMADYFNHW LAVGQRLADA GATLPRIYCV NWFRKGPNGK FVWPGFGENM RVLKWMLGRL 550 560 570 580 590 600 SGEAGGQEQV FGISPSYGDV DWTGLEFTPD QFQQVISVEA PAWREELALH GELFEQLAQG 610 620 LPPALSRAKA DIEHRLAAVQ A

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ49235.1.
RefSeq	YP_786146.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6265530.
GenomeReviews	Gene locus BAV1626 in contig AM167904_GR.
KEGG	bav:BAV1626.
NMPDR	fig|521.1.peg.1777.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2L1L0.
OMA	Q2L1L0. SHPNARF.
Family and domain databases
HAMAP	MF_00452. [Tree]
InterPro	IPR018091. PEP_carboxykin_GTP_CS. IPR013035. PEP_carboxykinase_C. IPR008209. PEP_carboxykinase_GTP. IPR008210. PEP_carboxykinase_N. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
PANTHER	PTHR11561. PEP_carboxykin. 1 hit.
Pfam	PF00821. PEPCK. 1 hit. [Graphical view]
PIRSF	PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
ProDom	PD004738. PEPCK_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00505. PEPCK_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKG_BORA1

Accession

Primary (citable) accession number: Q2L1L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)