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Q2L1L0 (PCKG_BORA1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckG
Ordered Locus Names:BAV1626
OrganismBordetella avium (strain 197N) [Complete proteome] [HAMAP]
Taxonomic identifier360910 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length621 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452

Subunit structure

Monomer By similarity. HAMAP MF_00452

Subcellular location

Cytoplasm By similarity HAMAP MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 621621Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452
PRO_1000060287

Regions

Nucleotide binding276 – 2816GTP By similarity
Nucleotide binding526 – 5294GTP By similarity
Region396 – 3983Substrate binding By similarity

Sites

Active site2771 By similarity
Metal binding2331Manganese By similarity
Metal binding2531Manganese By similarity
Metal binding3021Manganese By similarity
Binding site851Substrate By similarity
Binding site2261Substrate; via amide nitrogen By similarity
Binding site2331Substrate By similarity
Binding site2751Substrate By similarity
Binding site3981GTP By similarity
Binding site4291GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2L1L0 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 70C191E3B587F43F

FASTA62167,910
        10         20         30         40         50         60 
MTQAAEATLP PLNVPSYVKH ARLIDWVQGV VALTKPARVV WCDGSQEEAD RLCEQMVQAG 

        70         80         90        100        110        120 
TMRRLNPAKR PNSYLAWSDP SDVARVEDRT FICSQREEDA GPTNNWAAPA EMRNTLQGLF 

       130        140        150        160        170        180 
DGAMRGRTLY VVPFSMGPLG SPIAHIGVEL SDSPYVAVNM RIMTRMGRAV WDVLGADGEF 

       190        200        210        220        230        240 
VPCVHSVGMP LAEGQADVAW PCNPVKYIVH YPETREIWSF GSGYGGNALL GKKCFALRIA 

       250        260        270        280        290        300 
STMGRDEGWL AEHMLILGVT TPKGRKFHVA AAFPSACGKT NFAMLIPPSG MDGWKVSTIG 

       310        320        330        340        350        360 
DDIAWIKPGQ DGRLRAINPE AGYFGVAPGT SEKTNPNAMA TLKANVIFTN VALTDDGDVW 

       370        380        390        400        410        420 
WEGMTDTPPA HLIDWQGKDW TPEIARETGR KAAHPNARFT APASQCPSID PEWENPQGVA 

       430        440        450        460        470        480 
IDAFIFGGRR STTIPLVTEA RDWVQGVYMA ATMGSETTAA AVGQQGVVRR DPFAMLPFCG 

       490        500        510        520        530        540 
YNMADYFNHW LAVGQRLADA GATLPRIYCV NWFRKGPNGK FVWPGFGENM RVLKWMLGRL 

       550        560        570        580        590        600 
SGEAGGQEQV FGISPSYGDV DWTGLEFTPD QFQQVISVEA PAWREELALH GELFEQLAQG 

       610        620 
LPPALSRAKA DIEHRLAAVQ A

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References

[1]"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction."
Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M.
J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 197N.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM167904 Genomic DNA. Translation: CAJ49235.1.
RefSeqYP_786146.1. NC_010645.1.

3D structure databases

HSSPHSSP built from PDB template 2FAG based on UniProtKB P21642.
ProteinModelPortalQ2L1L0.
SMRQ2L1L0. Positions 17-617.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2L1L0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6265530.
GenomeReviewsGene locus BAV1626 in contig AM167904_GR.
KEGGbav:BAV1626.
NMPDRfig|521.1.peg.1777.
PATRIC21129428. VBIBorAvi43433_1636.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG484437.
OMACGYHMGD.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycABAU360910:BAV1626-MONOMER.

Family and domain databases

HAMAPMF_00452. PEPCK_GTP.
[Tree]
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KOK01596.
PANTHERPTHR11561. PEP_carboxykin. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_BORA1
AccessionPrimary (citable) accession number: Q2L1L0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 7, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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