Reviewed, UniProtKB/Swiss-Prot Q2KZF8 (PYRG_BORA1)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	BAV1165

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	CTP synthase HAMAP MF_01227		PRO_0000266072
Regions
	Domain	295 – 545	251	Glutamine amidotransferase type-1
	Region	1 – 257	257	Aminator domain HAMAP MF_01227
Sites
	Active site	383	1	Nucleophile By similarity
	Active site	518	1	By similarity
	Active site	520	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KZF8-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 7945F56D0AF90FC9	FASTA	550	60,655
	10 20 30 40 50 60 MTKYVFVTGG VVSSLGKGIA AASLAAILES RGLQVTLLKL DPYINVDPGT MSPFQHGEVF 70 80 90 100 110 120 VTEDGAETDL DLGHYERFIS TKMRRVNNFT TGQIYESVLR KERRGDYLGK TVQVIPHITN 130 140 150 160 170 180 EIQDFVARGA DSAWQGNTDV AIVEIGGTVG DIESLPFLEA ARQMSLRMGR NSAAFVHLTL 190 200 210 220 230 240 VPYIASAGEL KTKPTQHSVQ KLREIGIYPN ALLCRADRRI PDDERAKISM FSNVPLDAVI 250 260 270 280 290 300 SVWDVDSIYK IPAMLHQQGV DNIVCEALGL TPPPADLSMW DNLVDALEHP QHEVTIGMVG 310 320 330 340 350 360 KYVDLTESYK SLSEALVHAG IHTRSKVKIE YIDSEDIEAR GTEQLKHLDA ILVPGGFGKR 370 380 390 400 410 420 GTEGKIAAIR YARENGVPYL GICLGMQLAV IEFARHVAGL GGANSTEFDP SAPHPVVALI 430 440 450 460 470 480 TEWMDREGKV EKRDTNSDLG GTMRKGAQRC PIKPGTLAQS IYGDDVNERH RHRYEVNNVY 490 500 510 520 530 540 VPRLEAAGLV ISARTPTENL PEMMELPNHP WFVGVQFHPE FTSTPRDGHP LFSSYIRAAL 550 ESKNRRDPQA

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ48773.1.
RefSeq	YP_785687.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6265201.
GenomeReviews	Gene locus BAV1165 in contig AM167904_GR.
KEGG	bav:BAV1165.
NMPDR	fig|521.1.peg.2293.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KZF8.
OMA	Q2KZF8. EFNNAYR.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_BORA1

Accession

Primary (citable) accession number: Q2KZF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)