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Q2KYS4 (SYM_BORA1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:BAV0472
OrganismBordetella avium (strain 197N) [Complete proteome] [HAMAP]
Taxonomic identifier360910 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691Methionine--tRNA ligase HAMAP MF_00098
PRO_0000331785

Regions

Domain585 – 691107tRNA-binding
Motif12 – 2211"HIGH" region HAMAP MF_00098
Motif341 – 3455"KMSKS" region HAMAP MF_00098

Sites

Metal binding1431Zinc By similarity
Metal binding1461Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1591Zinc By similarity
Binding site3441ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2KYS4 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 53FDD38E678D7941

FASTA69176,712
        10         20         30         40         50         60 
MSRTLFVTTA LPYANGSFHI GHIMEYIQAD IWVRSMRMAG HTVHFVGADD AHGAPIMLKA 

        70         80         90        100        110        120 
EKEGITPQAL VARYAAERPR YLDGFHIRFD HWHSTDSAEN VALSHEIYRA LKAAGLIETR 

       130        140        150        160        170        180 
SIEQFFDPVK GMFLADRYIK GECPRCHAKD QYGDSCEVCG AVYAPTELIQ PYSALTGATP 

       190        200        210        220        230        240 
VLKRSDHFFF KLSDPRCVEF LQQWTTGSNR NGIKHLQSEV QAKTREWLGG EEGEAKLGDW 

       250        260        270        280        290        300 
DISRDAPYFG IEIPDAPGKY FYVWLDAPVG YLASLKSYCD KIGLDFDALL DPSSATEQVH 

       310        320        330        340        350        360 
FIGKDIIYFH ALFWPAMLKF AGRKTPDALN VHGFITVSGE KMSKSRGTGI SPLRYLEVGM 

       370        380        390        400        410        420 
DPEWLRYYMA AKLNARVEDM DFNPEDFIAR VNSDLVGKYV NIASRAANFI TRHFEGKLAY 

       430        440        450        460        470        480 
LGDTAALSAE FAQQTESIRA ALEAREFNRA IREIMAYADG INQAFDAAQP WVLAKGFAEA 

       490        500        510        520        530        540 
DEARRAQLQD ICSRALAGFK ALSVMLAPVL PALTSRVARD LFGAQRDFIW SDAADLPAQV 

       550        560        570        580        590        600 
APFKHLMQRV DPQMLDALFE PPAELAAPAP TPGGEALADT ISIDDFVKVD LRIAKIINCE 

       610        620        630        640        650        660 
EVEGSTKLLR LTLDAGEGRH RNVFSGIKSF YKPQDLIGKL TVLVANLAPR KMKFGVSEGM 

       670        680        690 
VLAASHADEK VDAGIYVLEP WPGAQPGMRI H

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References

[1]"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction."
Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M.
J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 197N.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM167904 Genomic DNA. Translation: CAJ48077.1.
RefSeqYP_785005.1. NC_010645.1.

3D structure databases

ProteinModelPortalQ2KYS4.
SMRQ2KYS4. Positions 2-560.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2KYS4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6264767.
GenomeReviewsGene locus BAV0472 in contig AM167904_GR.
KEGGbav:BAV0472.
NMPDRfig|521.1.peg.1067.
PATRIC21127092. VBIBorAvi43433_0482.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG721667.
OMAEEKTRNV.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycABAU360910:BAV0472-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BORA1
AccessionPrimary (citable) accession number: Q2KYS4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 7, 2006
Last modified: January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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