Reviewed, UniProtKB/Swiss-Prot Q2KYS4 (SYM_BORA1)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BAV0472

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331785
Regions
	Domain	585 – 691	107	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	341 – 345	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	344	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KYS4-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 53FDD38E678D7941	FASTA	691	76,712
	10 20 30 40 50 60 MSRTLFVTTA LPYANGSFHI GHIMEYIQAD IWVRSMRMAG HTVHFVGADD AHGAPIMLKA 70 80 90 100 110 120 EKEGITPQAL VARYAAERPR YLDGFHIRFD HWHSTDSAEN VALSHEIYRA LKAAGLIETR 130 140 150 160 170 180 SIEQFFDPVK GMFLADRYIK GECPRCHAKD QYGDSCEVCG AVYAPTELIQ PYSALTGATP 190 200 210 220 230 240 VLKRSDHFFF KLSDPRCVEF LQQWTTGSNR NGIKHLQSEV QAKTREWLGG EEGEAKLGDW 250 260 270 280 290 300 DISRDAPYFG IEIPDAPGKY FYVWLDAPVG YLASLKSYCD KIGLDFDALL DPSSATEQVH 310 320 330 340 350 360 FIGKDIIYFH ALFWPAMLKF AGRKTPDALN VHGFITVSGE KMSKSRGTGI SPLRYLEVGM 370 380 390 400 410 420 DPEWLRYYMA AKLNARVEDM DFNPEDFIAR VNSDLVGKYV NIASRAANFI TRHFEGKLAY 430 440 450 460 470 480 LGDTAALSAE FAQQTESIRA ALEAREFNRA IREIMAYADG INQAFDAAQP WVLAKGFAEA 490 500 510 520 530 540 DEARRAQLQD ICSRALAGFK ALSVMLAPVL PALTSRVARD LFGAQRDFIW SDAADLPAQV 550 560 570 580 590 600 APFKHLMQRV DPQMLDALFE PPAELAAPAP TPGGEALADT ISIDDFVKVD LRIAKIINCE 610 620 630 640 650 660 EVEGSTKLLR LTLDAGEGRH RNVFSGIKSF YKPQDLIGKL TVLVANLAPR KMKFGVSEGM 670 680 690 VLAASHADEK VDAGIYVLEP WPGAQPGMRI H

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM167904 Genomic DNA. Translation: CAJ48077.1.
RefSeq	YP_785005.1.
3D structure databases
SMR	Q2KYS4. Positions 2-560, 453-678, 581-690.
ModBase	Search...
Genome annotation databases
GeneID	6264767.
GenomeReviews	Gene locus BAV0472 in contig AM167904_GR.
KEGG	bav:BAV0472.
NMPDR	fig|521.1.peg.1067.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	SAYQPEQ.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BORA1

Accession

Primary (citable) accession number: Q2KYS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)