Reviewed, UniProtKB/Swiss-Prot Q2KYF3 (METE_BORA1)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BAV2306

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	766 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 766	766	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017227
Sites
	Metal binding	652	1	Zinc By similarity
	Metal binding	654	1	Zinc By similarity
	Metal binding	737	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KYF3-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 479F076F553311DB	FASTA	766	85,141
	10 20 30 40 50 60 MTITHNLGFP RIGAQRELKR AVEAYWAGKQ TADELLATGR ALRAAHWRRQ ADSGLRFVPV 70 80 90 100 110 120 GDFAWYDHIL EWTTLLGAVP ARFAQPETQA VSLDTLFRMG RGRAPSGKPA AACEMTKWFD 130 140 150 160 170 180 TNYHYIVPEL VPGQTYRIAR EDLFEQVREA QALGYAVKPV IPGPLTWLYL GKGDAFQQGA 190 200 210 220 230 240 SDEDKLKLLA NLLPVYQQVL KRLADQGVEW VQIDEPILAL DLPAAWRDAF GLAYAQLAGS 250 260 270 280 290 300 PVKVLLATYF DGLKDNLSTV TKLPVAGLHV DLVRAPEQLD DVVEALHADQ VLSAGVINGR 310 320 330 340 350 360 NVWRTDLDQA LRKLEPVARQ LGDRLWLAPS CSLLHVPVDL LAETELDAEL KSWLSFASQK 370 380 390 400 410 420 LDELSLLGRA LDGDSSQAVQ EGLAAQRAAL AARRSSPRIH NPAVGRRMAA SEQVSRDRAP 430 440 450 460 470 480 FAERIRQQQA RLNLPPFPTT TIGSFPQTAE IRGLRRDWKS GALSDSTYEA LIRKEIEAVI 490 500 510 520 530 540 RFQEKVGLDV LVHGEPERND MVEYFGELLA GFAFTKNGWV QSYGSRCVKP PIIFGDVARP 550 560 570 580 590 600 APMTVGWSSY AQSLTDKPVK GMLTGPVTIL QWSFVRDDQP REATCRQLAL ALRDEVLDLE 610 620 630 640 650 660 KAGVKVIQID EPAIREGLPL RRADWQAYLD WAVDCFRLST AGVADDTQIH THMCYSEFND 670 680 690 700 710 720 IIESIAAMDA DVITIETSRS NMELLKAFED FHYPNDIGPG VYDIHSPNVP DVDWMVGLMQ 730 740 750 760 KAGANLSKER LWVNPDCGLK TRAWPETEAA LISMVQAART LRQATS

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ49916.1.
RefSeq	YP_786820.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6265908.
GenomeReviews	Gene locus BAV2306 in contig AM167904_GR.
KEGG	bav:BAV2306.
NMPDR	fig|521.1.peg.131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KYF3.
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BORA1

Accession

Primary (citable) accession number: Q2KYF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)