Reviewed, UniProtKB/Swiss-Prot Q2KY72 (SYA_BORA1)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BAV2356

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 873	873	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347508

Sequences

	Sequence		Length	Mass (Da)
	Q2KY72-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 4A90B5F50954C261	FASTA	873	94,983
	10 20 30 40 50 60 MKSSEIRQKF LQFFQSKGHS IVPSSSLVPG NDPTLLFTNS GMVQFKDVFT GKEKRPYTRA 70 80 90 100 110 120 TSSQRSVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKQDAIRFAW ELLTTVYKLP 130 140 150 160 170 180 VEKLWVTVYQ EDDEAYDIWA KEVGVPTERI IRIGDNKGAR YASDNFWQMG DTGPCGPCTE 190 200 210 220 230 240 IFYDHGPDIW GGPPGSPEED GDRYIEIWNL VFMQFERDAA GNMTPLPKPC VDTGMGLERI 250 260 270 280 290 300 AAVLQGVHSN YEIDLFQKLI TAAARETGVQ DLHDNSLKVI ADHIRACSFL IVDGVIPSNE 310 320 330 340 350 360 GRGYVLRRIV RRALRHGYKL GQTKPFFYRL VPDLVAQMGE AYPELANMAE RVGQVLKQEE 370 380 390 400 410 420 ERFGETLEHG MKILDVALAQ VPKGGLLDGN TLFTLYDTYG FPVDLTADIC REREVEIDMA 430 440 450 460 470 480 GFEAAMERQR DQARAAGKFK MAEGLNYEGA QTRFEGYEQL ELSGAKVTAL YVDGTQTDQV 490 500 510 520 530 540 RAGQQAVVVL DATPFYAESG GQVGDTGLLV ADGLRFAVAD TLKVQAGVFG HHGLLEEGVL 550 560 570 580 590 600 KVGDTLLARV DAVRRARTVR NHSATHLMHK ALREVLGAHV QQRGSLVDPD KTRFDFAHDA 610 620 630 640 650 660 PMTAEQIARV EAIVNAEVLA NQAAQARVMA YDDAVKGGAM ALFGEKYGDT VRVLDIGFSR 670 680 690 700 710 720 ELCGGTHVSR TGDIGLFKIV SEGGVAAGVR RVEAITGDNA LVWVQEQNAL LQRAAGVLRS 730 740 750 760 770 780 PVAELPERIA QVQDQVKALE KDLEQARAKL AASAGNDLAD KSAVEIKGVK VLAAVINDVD 790 800 810 820 830 840 PKALRGMVDN LKDKLKPAIV LLAASADGKI SLVGGVTADQ TAKVKAGDLV GFVAAQVGGK 850 860 870 GGGRPDMAMG GGTDVAALPA AIASVQDWVN ERL

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ49966.1.
RefSeq	YP_786870.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6265931.
GenomeReviews	Gene locus BAV2356 in contig AM167904_GR.
KEGG	bav:BAV2356.
NMPDR	fig|521.1.peg.2283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KY72.
OMA	Q2KY72. TLMFTNS.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BORA1

Accession

Primary (citable) accession number: Q2KY72

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)