Reviewed, UniProtKB/Swiss-Prot Q2KXE5 (SYL_BORA1)

Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	BAV2492

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subcellular location	Cytoplasm. HAMAP MF_00049
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	Leucyl-tRNA synthetase HAMAP MF_00049		PRO_1000009298
Regions
	Motif	48 – 58	11	"HIGH" region HAMAP MF_00049
	Motif	639 – 643	5	"KMSKS" region HAMAP MF_00049
Sites
	Binding site	642	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KXE5-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: A55D8D4B863156EF	FASTA	885	99,022
	10 20 30 40 50 60 MQERYQPNLV EAAAQQDWQA RDAYLVHESA KNADGSEKPK FYACSMLPYP SGKLHMGHVR 70 80 90 100 110 120 NYTINDMMAR QLRMRGYNVL MPMGWDAFGM PAENAAIKSK VPPAKWTYDN IAYMKKQMKA 130 140 150 160 170 180 MGLAIDWSRE MCACDPAYYK WNQWLFLKML EKGIAYRKTQ VVNWDPVDQT VLANEQVIDG 190 200 210 220 230 240 RGWRSGAPVE KREIPGYYLR ITDYADELLD QVKNGLPGWP ERVRVMQENW IGKSEGVRLA 250 260 270 280 290 300 FPHDIKDENG QLIQDGKLFV FTTRADTVMG VTFCAVAPEH PLATLAARNN PALATFIEQC 310 320 330 340 350 360 KLGGTTEAEI ATREKEGIPT GLSVKHPLTG QAVDLWVGNY VLMSYGDGAV MGVPAHDERD 370 380 390 400 410 420 FAFARKYGLT IRQVIAQEGK TYSDQAWQEW YGDKQTGRTI NSGKYDGLST AEAVDAIAAD 430 440 450 460 470 480 LNALGLGEKQ TTYRLRDWGI SRQRYWGTPI PIIHCQDCGP VPVPEQDLPV VLPDDLIPDG 490 500 510 520 530 540 SGNPLAKNEA FLSCSCPACG KPARRETDTM DTFVDSSWYF MRYTSPGNDQ AMVDKRNDYW 550 560 570 580 590 600 MPMDQYIGGI EHAVLHLLYA RFWTKVMRDL GMLKFDEPFT RLLCQGMVLN HIYSRRTPQG 610 620 630 640 650 660 GIEYFWPEEV ENIYDAKGAI VGARLKSDGS EITYGGVGTM SKSKNNGVDP QSLIDTLGAD 670 680 690 700 710 720 TARLFVMFAS PPEQTLEWSD SGVDGANRFL RRLWALAYDR RAAVARGLAS GYAWQDAPAP 730 740 750 760 770 780 VKDLRRELYG LLKQAEYDYQ RIQYNTVVSA SMKMLNAIDN AQLPEGPAAD AAIAEGLGLL 790 800 810 820 830 840 LRVLYPVVPH VTWHIWRDLG YAAELGDLLD APWPHVDEAA LIADEIELML QVNGKLRGAI 850 860 870 880 RVAAQAAKED IEKIAVAQEE VARFLEGRPP KRVIVVPGKL VNVVG

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ50102.1.
RefSeq	YP_787004.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6266007.
GenomeReviews	Gene locus BAV2492 in contig AM167904_GR.
KEGG	bav:BAV2492.
NMPDR	fig|521.1.peg.6.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KXE5.
OMA	Q2KXE5. ATREKEG.
Family and domain databases
HAMAP	MF_00049. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_BORA1

Accession

Primary (citable) accession number: Q2KXE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)