Reviewed, UniProtKB/Swiss-Prot Q2KXE0 (PROA_BORA1)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	BAV2495

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000252564

Sequences

	Sequence		Length	Mass (Da)
	Q2KXE0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: FD66A8A0D0A16518	FASTA	419	44,373
	10 20 30 40 50 60 MSSIEQIMLT LGENARQASR AMMRASGTAK NRALLAMAEL IIDGKAALQA ANALDLEAAR 70 80 90 100 110 120 ANGLEPALLD RLTLSDRSVQ LMAEGLRQIA ALPDPVGSLG PTQVRPNGMR VAQMRVPLGV 130 140 150 160 170 180 IGIIYESRPN VTIDAAALCL KSGNATILRG GSEALHSNLA LGAIVQAGLA AAELPPAAVQ 190 200 210 220 230 240 VVSTTDRAAV GKLVTMTEHI DVIVPRGGKG LIARLAQEAR VPLIKHLDGN CHVYIDAAAD 250 260 270 280 290 300 PDRALEIAFN AKTYRYGVCG SMETLLVHRA IAPTQLPRIA RALIEHGVEL RGCERSQAIV 310 320 330 340 350 360 PGMAAASEED WGTEYLGPVL AVRVVDDLDQ AMEHIARWGS GHTDAIVTEN LAAAQRFQRE 370 380 390 400 410 VDSSSVYVNL PTVFADGFEY GLGAEIGIST NRLHARGPVG LEGLTTYKWV LTGEGQTRG

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ50105.1.
RefSeq	YP_787007.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6267189.
GenomeReviews	Gene locus BAV2495 in contig AM167904_GR.
KEGG	bav:BAV2495.
NMPDR	fig|521.1.peg.3.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KXE0.
OMA	Q2KXE0. FDTEWLD.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BORA1

Accession

Primary (citable) accession number: Q2KXE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)