ID   MIAA_BORA1              Reviewed;         312 AA.
AC   Q2KX23;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=tRNA Delta(2)-isopentenylpyrophosphate transferase;
DE            Short=IPP transferase;
DE            EC=2.5.1.8;
DE   AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
DE            Short=IPTase;
DE            Short=IPPT;
DE   AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
DE            Short=DMAPP:tRNA dimethylallyltransferase;
DE            Short=DMATase;
GN   Name=miaA; OrderedLocusNames=BAV0724;
OS   Bordetella avium (strain 197N).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=360910;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16885469; DOI=10.1128/JB.01927-05;
RA   Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D.,
RA   King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D.,
RA   Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R.,
RA   Squares S., Woodward J., Parkhill J., Temple L.M.;
RT   "Comparison of the genome sequence of the poultry pathogen Bordetella
RT   avium with those of B. bronchiseptica, B. pertussis, and B.
RT   parapertussis reveals extensive diversity in surface structures
RT   associated with host interaction.";
RL   J. Bacteriol. 188:6002-6015(2006).
CC   -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC       adenine at position 37 in tRNAs that read codons beginning with
CC       uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC       (i(6)A) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate +
CC       tRNA containing 6-isopentenyladenosine.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the IPP transferase family.
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DR   EMBL; AM167904; CAJ48335.1; -; Genomic_DNA.
DR   RefSeq; YP_785257.1; -.
DR   GeneID; 6267599; -.
DR   GenomeReviews; AM167904_GR; BAV0724.
DR   KEGG; bav:BAV0724; -.
DR   NMPDR; fig|521.1.peg.899; -.
DR   HOGENOM; Q2KX23; -.
DR   OMA; Q2KX23; VNADSMQ.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004811; F:tRNA isopentenyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008033; P:tRNA processing; IEA:HAMAP.
DR   HAMAP; MF_00185; -; 1.
DR   InterPro; IPR002627; IPPT.
DR   InterPro; IPR018022; tRNA_delta_PyrP_Trfase.
DR   PANTHER; PTHR11088; IPPT; 1.
DR   Pfam; PF01715; IPPT; 1.
DR   ProDom; PD004674; IPPT; 1.
DR   ProDom; PD005388; IPPtrans_like; 1.
DR   TIGRFAMs; TIGR00174; miaA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Magnesium; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase; tRNA processing.
FT   CHAIN         1    312       tRNA Delta(2)-isopentenylpyrophosphate
FT                                transferase.
FT                                /FTId=PRO_1000020565.
FT   NP_BIND      11     18       ATP (Potential).
FT   REGION       13     18       Substrate binding (By similarity).
FT   REGION       36     39       Interaction with substrate tRNA (By
FT                                similarity).
FT   REGION      160    164       Interaction with substrate tRNA (By
FT                                similarity).
FT   REGION      243    248       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        102    102       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        124    124       Interaction with substrate tRNA (By
FT                                similarity).
SQ   SEQUENCE   312 AA;  33927 MW;  1CC8244E91D49FAF CRC64;
     MNTPPLICLA GPTAAGKSAA TLALAERWPL EIINVDSATI YRGMDIGTAK PSAAEQAQVA
     QHLLDIRDPS QAYSAADFRT DTLALIEDIQ ARGRIPLLAG GTMLYYKALR EGLDDLPQAD
     PVLRAELEAR AANEGWPALH AELARHDPIT AARLSPNDSQ RIQRALEVCL LSGRAMSALL
     TGTRRPAPSD LRFVTISLEP SDRAGLHARI AQRFDAMLQA GLEAEVRSLK QRTDLHPGLP
     SVRCVGYRQM WAYLDGEVSF DEAREQGIAA TRQLAKRQLT WLRAQPERVI VDCLAAGTAA
     RVVDIAARYL PG
//