Reviewed, UniProtKB/Swiss-Prot Q2KWX0 (AMPA_BORA1)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BAV1137

Organism

Bordetella avium (strain 197N) [Complete proteome] [HAMAP]

Taxonomic identifier

360910 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019886
Sites
	Active site	288	1	Potential
	Active site	362	1	Potential
	Metal binding	276	1	Manganese 2 By similarity
	Metal binding	281	1	Manganese 1 By similarity
	Metal binding	281	1	Manganese 2 By similarity
	Metal binding	299	1	Manganese 2 By similarity
	Metal binding	358	1	Manganese 1 By similarity
	Metal binding	360	1	Manganese 1 By similarity
	Metal binding	360	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2KWX0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: D3882F19606A16D1	FASTA	504	52,892
	10 20 30 40 50 60 MEFSTQTTAS LHLIKTSALA VGVYADGVLS PAAEQIDHAS NGAIRAVTKT EFRGRAGATL 70 80 90 100 110 120 VLRNLAGISA QRVVLVGLGK QEEYSVRAHS GAEQAFAAYL VAAQLTEGVS TLAALPIENS 130 140 150 160 170 180 TMRDRARAAA IAAGQATYHY DATFGKPDRE ALPKLKKITQ IIERAEAAQT QQGLREGAAI 190 200 210 220 230 240 ANGMALTRTL GNLPGNICTP TYLGETARKL AREFKTLIKV EVLDRKQVEA LGMGSFVSVA 250 260 270 280 290 300 RGSAEPLRFV VLRYNGKPAT ARRTRGAAGP VVLVGKGITF DAGGISIKPA ATMDEMKYDM 310 320 330 340 350 360 CGAASVLGTF RALAELAPAL EVVGLIAACE NLPSGTANKP GDVVTSMSGQ TIEILNTDAE 370 380 390 400 410 420 GRLVLCDALT YAERFKPSAV IDIATLTGAC VVALGGVNTG LFSKDDALAS ALLEAGRQTQ 430 440 450 460 470 480 DPAWRMPLDD AYQEQLRSNF ADIANIGGPQ AGAVTAACFL SRFTQAYPWA HLDIAGTAWR 490 500 GGKDKGATGR PVPLLMQYLL NQAA

References

[1]

"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction." Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M. J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM167904 Genomic DNA. Translation: CAJ48746.1.
RefSeq	YP_785660.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	6267191.
GenomeReviews	Gene locus BAV1137 in contig AM167904_GR.
KEGG	bav:BAV1137.
NMPDR	fig|521.1.peg.615.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2KWX0.
OMA	LGHHNSG.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BORA1

Accession

Primary (citable) accession number: Q2KWX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)