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Q2KWI6 (PNP_BORA1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:BAV2667
OrganismBordetella avium (strain 197N) [Complete proteome] [HAMAP]
Taxonomic identifier360910 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595

Subunit structure

Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

mRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5'-exoribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyribonucleotide nucleotidyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Polyribonucleotide nucleotidyltransferase HAMAP MF_01595
PRO_0000329535

Regions

Domain558 – 61760KH
Domain627 – 69569S1 motif

Sequences

Sequence LengthMass (Da)Tools
Q2KWI6 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: 01E0C1EF0D565AC3

FASTA71877,312
        10         20         30         40         50         60 
MFNKVTKTFQ YGQHTVVLET GEIARQASGA VLVSVEDTVV LATVVAAKKA KPGQDFFPLT 

        70         80         90        100        110        120 
VDYIEKTYAA GRIPGGFFKR EGKPSEKETL TSRLIDRPLR PLFPEGFYNE VQVVIHTLSV 

       130        140        150        160        170        180 
NPEIDPDIPA MIGASAALAI SGIPFNGPIG AARVAYVEGQ YLLNPTASQL KSSKMDLVVA 

       190        200        210        220        230        240 
GTENAVLMVE SEAQQLSEEI MLGGVVFGHE QMQAAINAIH DLVRDAGKPE WTWSPAAKNE 

       250        260        270        280        290        300 
ALIAAVTAAA QEGLSAAYQI REKQARTTKL REVYAEVSAK LAEQAAAAGQ DAPDGVTVDN 

       310        320        330        340        350        360 
ILFDLEARLV RSQILNGEPR IDGRDTRTVR PISVRLGVLP RAHGSALFTR GETQALVVAT 

       370        380        390        400        410        420 
LGTKQDEQII DALMGEYRDR FMLHYNMPPF ATGETGRIGV PKRREIGHGR LAKRSLVPVL 

       430        440        450        460        470        480 
PKPEDFQYTI RIVSEITESN GSSSMASVCG GSLAMMDAGV PITDHVAGVA MGLILENGKF 

       490        500        510        520        530        540 
AVLTDILGDE DHLGDMDFKV AGTENGITAL QMDIKIQGIT KEIMQVALAQ AREGRLHILG 

       550        560        570        580        590        600 
KMRESLDGSR TELSAFAPRM LTIKINPEKI RDVIGKGGAT IRALTEETGT QIDISDDGTI 

       610        620        630        640        650        660 
VIASVDEGQA KEAQRRIVEL TADVEVGQVY DGSVLRLLDF GAIVQVLPGR DGLLHISEIA 

       670        680        690        700        710 
NYRIANINDV LKVGQQVRVK VIEADEKGRL RLSVKAIGGI EQQQAAADVP AQAETPAE

« Hide

References

[1]"Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction."
Sebaihia M., Preston A., Maskell D.J., Kuzmiak H., Connell T.D., King N.D., Orndorff P.E., Miyamoto D.M., Thomson N.R., Harris D., Goble A., Lord A., Murphy L., Quail M.A., Rutter S., Squares R., Squares S., Woodward J., Parkhill J., Temple L.M.
J. Bacteriol. 188:6002-6015(2006) [PubMed: 16885469] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 197N.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM167904 Genomic DNA. Translation: CAJ50278.1.
RefSeqYP_787166.1. NC_010645.1.

3D structure databases

ProteinModelPortalQ2KWI6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2KWI6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6266139.
GenomeReviewsGene locus BAV2667 in contig AM167904_GR.
KEGGbav:BAV2667.
NMPDRfig|521.1.peg.291.
PATRIC21131611. VBIBorAvi43433_2697.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG382411.
OMAYGETVVL.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycABAU360910:BAV2667-MONOMER.

Family and domain databases

HAMAPMF_01595. PNPase.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR012162. PNPase.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
KOK00962.
PANTHERPTHR11252. PNPase. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF46915. 3_ExoRNase. 1 hit.
SSF55666. 3_ExoRNase. 2 hits.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMsTIGR03591. Polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_BORA1
AccessionPrimary (citable) accession number: Q2KWI6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 7, 2006
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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